tetano
Editor, Senior Moderator
Biomed Res Int. 2014;2014:904038. doi: 10.1155/2014/904038. Epub 2014 May 14.
Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 TatΔ51-59 Protein in Mice.
Garulli B1, Di Mario G2, Stillitano MG2, Compagnoni D3, Titti F3, Cafaro A3, Ensoli B3, Kawaoka Y4, Castrucci MR2.
Author information
Abstract
Recombinant influenza viruses hold promise as vectors for vaccines to prevent transmission of mucosal pathogens. In this study, we generated a recombinant WSN/TatΔ51-59 virus in which Tat protein lacking residues 51 to 59 of the basic domain was inserted into the N-terminus of the hemagglutinin (HA) of A/WSN/33 virus. The TatΔ51-59 insertion into the viral HA caused a 2-log reduction in viral titers in cell culture, compared with the parental A/WSN/33 virus, and severely affected virus replication in vivo. Nevertheless, Tat-specific antibodies and T cell responses were elicited upon a single intranasal immunization of BALB/c mice with WSN/TatΔ51-59 virus. Moreover, Tat-specific immune responses were also detected following vaccine administration via the vaginal route. These data provide further evidence that moderately large HIV antigens can be delivered by chimeric HA constructs and elicit specific immune responses, thus increasing the options for the potential use of recombinant influenza viruses, and their derivatives, for prophylactic and therapeutic vaccines.
PMID:
24949479
[PubMed - in process]
PMCID:
PMC4053076
Free PMC Article
http://www.ncbi.nlm.nih.gov/pubmed/24949479
Induction of Antibodies and T Cell Responses by a Recombinant Influenza Virus Carrying an HIV-1 TatΔ51-59 Protein in Mice.
Garulli B1, Di Mario G2, Stillitano MG2, Compagnoni D3, Titti F3, Cafaro A3, Ensoli B3, Kawaoka Y4, Castrucci MR2.
Author information
Abstract
Recombinant influenza viruses hold promise as vectors for vaccines to prevent transmission of mucosal pathogens. In this study, we generated a recombinant WSN/TatΔ51-59 virus in which Tat protein lacking residues 51 to 59 of the basic domain was inserted into the N-terminus of the hemagglutinin (HA) of A/WSN/33 virus. The TatΔ51-59 insertion into the viral HA caused a 2-log reduction in viral titers in cell culture, compared with the parental A/WSN/33 virus, and severely affected virus replication in vivo. Nevertheless, Tat-specific antibodies and T cell responses were elicited upon a single intranasal immunization of BALB/c mice with WSN/TatΔ51-59 virus. Moreover, Tat-specific immune responses were also detected following vaccine administration via the vaginal route. These data provide further evidence that moderately large HIV antigens can be delivered by chimeric HA constructs and elicit specific immune responses, thus increasing the options for the potential use of recombinant influenza viruses, and their derivatives, for prophylactic and therapeutic vaccines.
PMID:
24949479
[PubMed - in process]
PMCID:
PMC4053076
Free PMC Article
http://www.ncbi.nlm.nih.gov/pubmed/24949479