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INDONESIA - BF in PIGS in Bali

Re: INDONESIA - BF in PIGS in Bali

Reviewing details of the ProMed article...

...found evidence
that the avian influenza (AI) virus has infected pigs in Bali...
Out of 20 pigs, 2 were positively infected by H5N1 virus.
the H5N1 virus was not found in the animals' tissue. "Probably the
virus only passed by, or is called an opportunistic virus...

So was the H5N1 found in something besides tissue? Blood, GI tract, nose, lungs, feces?

.
 
Re: INDONESIA - BF in PIGS in Bali

Reuters is picking up the story.., official does not know where he is talking about?.. research is from 2005, published several times in the local media from june 2006.., may be I am wrong...and more contaminated pigs were found, not reported yet...

JAKARTA (Reuters) - The H5N1 bird flu virus has infected pigs on the Indonesian resort island of Bali, a senior agriculture ministry official said on Monday.

"There were two pigs that were infected by bird flu in Bali. These were old cases that happened last July," Musni Suatmodjo, agriculture ministry director of animal health, told Reuters.

Koran Tempo newspaper had reported on the weekend that a team from the veterinary faculty at Udayana University had discovered avian influenza infected two pigs in the regencies of Gianyar and Tabanan in Bali. it was not clear if the pigs died.

Pigs are a concern because they are susceptible to many of the viruses that infect humans. Swines can act as mixing vessels in which genetic material from avian flu viruses can mix with human influenza viruses, potentially producing new and deadly strains for which humans have no immunity.
I Gusti Putu Suwandi, head of the Tabanan agriculture office, said there have not been new cases of avian influenza in the area since July.

"As for the pigs' cases, we haven't received a formal report of the finding from the university," Suwandi told Reuters by telephone.

(edit: report was send to the local veterinary authorities:"The discovery was not yet published as the scientific study, but has been sent to the Balinese Livestock Breeding Service as the warning to be guarded against." http://www.flutrackers.com/forum/showpost.php?p=36871&postcount=13

The agriculture ministry's Suatmodjo said bird flu had been detected in 30 out of 33 provinces in the country, with the latest cases in North Sulawesi province. He said that although that was an increase from 29 provinces last year, the percentage of deaths in poultry was lower thanks to better vaccination and other control measures.
.
"The number of death cases in poultry due to bird flu were relatively small as commercial farms have done proper vaccination and biosecurity, but the main problems remain on the backyard farms," Suatmodjo said, referring to the many Indonesians who keep a handful of chickens at their homes.
Indonesia has become one of the frontlines in the battle against the disease. So far, 52 people have died of bird flu, the highest of any country, with the majority of deaths occurring since the beginning of this year.
Worldwide, 148 people have died of bird flu since 2003.
Although the human death toll has climbed, the Indonesian government has resisted mass culling of birds, citing the expense and impracticality in a huge, populous country where keeping a few chickens or ducks in backyards is common.

Culling at selective farms and their immediate surroundings has been the preferred method.
Millions of chickens and other fowl in Indonesia have died from the disease or been killed to prevent its spread since it first surfaced in the archipelago in late 2003.

New link:
http://today.reuters.com/news/artic..._JAK269156_RTRUKOC_0_US-BIRDFLU-INDONESIA.xml
 
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Re: INDONESIA - BF in PIGS in Bali

Interesting details about the research on Bali, genetic information available

The virus sprouted in the Pig (Bali)

on Thursday, October 12 2006 | 01:02 WIB


There is none that could the implementation more far to rescue his pigs that were infected Hog Cholera.During so -last June- a girl of the veterinary surgeon's candidate from the Udayana University that was the spacious practice offered a sheet of Rp 100 thousands for one around his pig that a rust.

"I needed him to do the task of the end of the lecturer," said the girl who later it was known was named Natalya Ruff, 26 years.Natalya brought the pig to his campus laboratory in Denpasar.

The first step that the implementation was to take the sample of the liquid on the throat of the unfortunate pig used cotton swap.The previous sample was analysed by him technically the Reaction of the Polimerase Chain (PCR).
Natalya blood instantly terkesiap when under his microscope lens, he not only found the virus hog cholera, but also a descendant of the virus that was pursued throughout the country -in fact the world- like the large-scale class fugitive, H5N1.

"Deg-degan also at that time but not at all frightened because of feeling quite safe," said Natalya that had a pointed nose that.Natalya immediately announced the findings of the bird flu virus to his guide's lecturer, IGN Ketut Mahardika.Together, both of them operated on the pig to be researched by the possibility of the descendants of the same virus merasuk right in the network of blood and the organ.

For the further test of Mahardika -the dropout's doctor Giessen University, Germany- divided the data and the sample to the colleagues in the similar laboratory in Jakarta (the Molecular Eijkman biological Agency), Bogor (the Bogor agricultural Institute) and Singapore (National University).

Results evidently the negative."That was significant to the pig in Tabanan the bird flu virus was oportunistik, or only passinged by," explained Mahardika.

The characteristics were different to the other pig 'patient' who came to Mahardika almost simultaneously.This time, his guidance student that the spacious practice in Payangan, Gianyar, Athens R. Dion, brought the present of the pig child was three months old.

The Landrace kind pig was stunted, dehydration, anaemia, diarrhoea and breathless for the last month.According to Athens, your four tails the pig child that was born berbarengan already more previously died.

Results of the sample test technically PCR again proved the pig is junior that had been descended on by the virus H5N1.Even not only alighted because of the test imunohistokimia with antibodies monoklonal demonstrated if virus antigens H5N1 lived especially in the network of the spleen.

According to Mahardika, the genetic material comparison the gene hemaglutinin (HA, protein in the virus that was assigned to ambush the network of his foster-mother's body) the virus to two pigs showed the difference although very thin.

Genetic information cDNA the gene HA that was available from isolat the Gianyar pig was 1489 nukleotida was the Tabanan pig 1397 nukelotida.

"Both of them are currently registered in the Bank Gen in America (belonging to National the Institute of Health)," he said.


http://www.tempointeraktif.com/hg/iptek/2006/10/12/brk,20061012-85877,id.html

Credits Imersion CE
 
Re: INDONESIA - BF in PIGS in Bali

toggletext-ed from Indonesian:

The Provincial Government was not sure Bird Flu infected Babi
Oct 26, 2006

Denpasar - The Section Head Balinese Province Livestock Breeding, Ir Ida Bagus Raka not yet believe in that bird flu infected the pig as being found by the team of the Medical Faculty the Animal (FKH) Unud. Raka precisely claims up to now to be his side did not yet get the report on the existence of the pig that died the reason for the virus attack H5N1 that. :rolleyes: "Till at this time in Bali did not yet have bird flu infected the pig. That would just the temporary assumption of the team of the researcher Unud. We with BPPV also has taken sample, but not yet in the conclusion of the existence jangkitan to the pig," said Raka toned denied it, to this newspaper, yesterday. His side will only trust results of the laboratory that the implementation personally. Nevertheless his side continued to guard against the possibility of the occurrence of the mutation of bird flu to the other animal. "Our target precisely made Bali the free area of bird flu," he stated. Because that of various anticipation was carried out. As expediting the vaccination against the poultry and the banning of the entry of the poultry from outside Bali.

He mentioned in the budget of the previous change has been budgeted for the Rp fund 500 million for the control of this bird flu. Moreover sped up the socialisation of pen sanitation to the corner of the village, especially to the developer of the poultry business. His side admitted to continuing to prepare the cadre PDR and PDS as the team of the socialisation to mencapaikan exactly and accurately concerning cirri-characteristics and the prevention of the pattern of the distribution of bird flu. Responded to the impact of the banning on chicken supplies in Bali, Raka said, the Provincial Government only banned the entry of the living chicken. While the chicken that has been cut off in the form of the carcass was not forbidden to enter the origin has been guaranteed by the related side in the entrance. About the fact that chicken supplies not all that 20 thousand tails to satisfy the requirement for the market, his side expected that resulting from the jump of the price of food and the seed so as suplaer could not fill the quota of supplies.

"Frank that was minded by us was the Balinese tourism image, because if rumours of bird flu spread would more our bustle developed again this tourism," obviously him. Added, the Balinese liberation program from the AI illness (Avian influenza) will be proclaimed from this year end. And intensively his handling will be carried out from this coming 2007.

http://www.jawapos.co.id/index.php?act=detail_radar&id=141901&c=94
 
Re: INDONESIA - BF in PIGS in Bali

Jakarta Post:Possibility of avian influenza epidemic a worry

Interview with Mahardika, the researcher on Bali


Possibility of avian influenza epidemic a worry

I Wayan Juniartha, The Jakarta Post, Denpasar

It seems that Bali is not only a paradise for vacationers, but also for H5N1, the dangerous virus known as avian influenza, or bird flu.

While visitors seek a memorable experience, the virus is struggling simply to ensure the survival of its kind.

It may not be appropriate to bracket the leisure of the tourism industry with the menace of the avian influenza epidemic. It certainly is not a pretty comparison.

"Pretty or not, it is a fact that we are all have to deal with right now," Ngurah Mahardika stated.

Mahardika is the team leader of the Avian Influenza Detection and Identification Program, an ongoing, government-funded project carried out by the school of veterinary medicine at Udayana University.

Since late 2005, Mahardika and his team have conducted extensive surveys in Bali, West Nusa Tenggara and East Nusa Tenggara, searching for the dangerous virus.


The results are scary: The virus has infected poultry populations in 37 of the total 55 subdistricts in Bali. "We took samples in 152 villages and we found infected poultry population in 53 villages," he said.

The bad news didn't stop there. Recently, following a series of tests on 800 pigs across the island, his team has identified over 20 pigs infected by the virus. Back in 2005, when they tested 400 pigs, no infection was found.

Being a virus expert -- he holds a PhD in molecular virology from Germany's Justus-Leibig University -- Mahardika knows perfectly well the dangerous implications of that recent development.

"It means that the virus has succeed in inching closer to the human population," he said.

So far, there hasn't been any reported case of the virus infecting the human population of the island. Yet, it is a grave, tragic error to think that it will forever ignore an opportunity to infect a new host.

"Honestly speaking, I'm now very worried about such a possibility," he confessed.

Unfortunately, the majority of the island's human population still does not view avian influenza as an immediate, growing threat.

The island is not properly prepared to tackle a possible avian influenza epidemic.

"The problem is the prevailing atmosphere of indifference. Many people have mistakenly assumed that avian influenza does not pose a grave threat to the island," he said.

Sadly, such indifference has also persisted among the majority of the island's media outlets, public institutions and government agencies.

Their levels of apathy are not similar, but the end result is the same.

The general public's lack of awareness of avian influenza, the lack of effective, standardized and island-wide detection and prevention measures, and the lack of an emergency response management are some of the results of such apathy.

"We don't have any kind of scenario, let alone a worst-case one. We don't know which parts of the island are the most vulnerable, the speed of the viral transmission or how many people will have been infected before we will be able to effectively contain the epidemic," said a doctor, who declined to be named.

Being the only health service provider in a rural village in northern Badung, he was quite afraid that the village didn't stand a chance against the epidemic.

"There are a large number of chicken farms in the village. The human population is quite dense. It's not rocket science to realize that this village is vulnerable to the epidemic," he said.

"At the same time, we don't have enough manpower, facilities and medical supplies to carry out an emergency operation if there is an outbreak of avian influenza," he explained.

To a large extent, the village is a miniature version of the island. The poultry population on the island totals over 12 million while the pig population is around 900,000.

The island is also populated by 3.4 million people.

"Bali is densely populated by poultry, pigs and humans: All have been positively identified as possible hosts for H5N1," Mahardika said.

"Bali bears disturbing similarities to various dense areas in China. Such density provides the virus with a paradise, where they could duplicate as many times as they wish," he added.


The density factor is further aggravated by two particular practices commonly employed by poultry and pig farmers in Bali.

One practice -- letting ducks search for food in newly harvested paddy fields -- exposes the ducks to probable transmission from migratory wild birds.

"Paddy fields are the primary source of food for migratory wild birds. The World Health Organization (WHO) has identified such creatures as the primary agent of transmission of the avian influenza among poultry populations," Mahardika stated.

Moreoever, Tumpang Sari, a practice of keeping pigs and poultry in the same spot, has increased the possibility of the virus "jumping" from one species to another -- poultry to pig.

"The danger of having the virus in pigs is that the pig could serve as a host for various subtypes of the virus," he said.

"In pigs, the subtypes could also get involved in a 'reassortment' process to adapt themselves with the new non-poultry host," he said.

Moreover, it has been known that pigs could also be infected by the human influenza virus. If avian and human viruses are mixed and reassorted inside the pig, the results could be devastating.

They will either produce a more powerful version of the old viruses or new viruses with a lethal ability to penetrate the barriers.

"The mixing and reassortment process will make it easier for the virus to get into the next available host -- human beings," he pointed out.

The only hope, Mahardika believed, lies in community-based prevention and detection programs. In the absence of an island-wide, government-initiated prevention measure, the powerful traditional institutions, such as desa pekraman (traditional villages) and banjar (neighborhood organizations), are the only institutions that could mobilize the Balinese to act in unison.

"The key to dealing with an epidemic is detection and prevention. We must educate and empower the traditional institutions to perform those actions," he said.

Moreover, pecalang (traditional security guards) could be assigned with another responsibility: supervising the poultry and pig farms and keeping a close tab on poultry trade and transport.

The role of pecalang will be particularly significant since the government's much-publicized measure of prohibiting the transportation of live poultry into the island ended in failure.

Through bribery and ingenuity, the poultry traders managed to send poultry from Java and Lombok into Bali on a regular basis. "Every day they unload live poultry from Java in a street in west Denpasar," said Putu Tapa, a resident of that area.

Mahardika believes that the education and empowerment of the traditional institutions will play a critical role in warding off the threat.

"With support from those institutions there is a huge possibility that we will be able to prevent the occurrence of an outbreak or confine the outbreak to a limited geographical area," he said.

Education and empowerment are effective ways to wipe out the general public's indifference toward the threat of avian influenza.

After all, public indifference is borne out of a lack of knowledge.

Mahardika, however, still has to deal with another kind of indifference; that which has been borne out of fear and greed.

Many, including government officials, have accused him of tainting the island's image as a prime tourist destination. They argue that Mahardika has made a lot of fuss over an empty threat and, by doing so, had damaged the island's tourism industry.

"They are afraid that tourists will not visit the island if they know about the possibility of an avian influenza outbreak," Mahardika said.

Mahardika doesn't see it that way. As a responsible host for tourists, he argued, the Balinese should make sure that their guests are well-protected from any harm.

"If we can show the world that we have taken any necessary precaution to protect the island and its human population -- the foreigners as well as the natives -- I believe that it will boost, instead of degrade, the image of our tourism industry," he said.

Denials and cover-ups, on the other hand, are simply fruitless.

"History has taught us that viral epidemics move swiftly, mercilessly and indiscriminately. If such an epidemic takes place in Bali, even the mightiest of the tourism industry's public relation agency will not be able to salvage the island's image," he warned.

http://www.thejakartapost.com/detailfeatures.asp?fileid=20061102.T01&irec=0
 
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Re: INDONESIA - BF in PIGS in Bali

Mahardika again..

Sharp end in Bali's war against possible bird flu outbreak

Inside a dilapidated two-story building in a dusty corner of Udayana University campus in Denpasar, lies the Veterinary Medicine Department's molecular biology laboratory, the sharp end of the island's fight against avian influenza, or bird flu.

Prior to December 2005, a sample from a suspected infected animal had to be sent to Jakarta or Hong Kong for positive viral identification, a process that could take up to a week.

Currently, this lab can complete the process in a day or less. Moreover, it can process up to 300 samples at a time.

"Give us a sample in the morning and by the afternoon we will give you the result, which includes the specific subtype of the avian influenza virus," the lab's head Ngurah Mahardika said. From the perspective of prevention, the reduction from one week to one day is very significant.

"Imagine what the epidemic could do in six days to a tiny, densely populated island like Bali; and, what we can do in six days," Mahardika said.

"Those six days are the line that separates life-saving measures from life-threatening epidemic," he stressed.

The laboratory contains a three meter by five meter glass chamber, where most of the dangerous work of preparing the virus for identification takes place.

Two centrifuges, an ELISA reader and a thermocycler stand on a table at the chamber's north section. Facing the table is a Laminar flow cabinet and a laboratory-grade oven.

Currently, several additional chambers are being constructed to upgrade the laboratory's capability and increase its bio-security level.

Pointing at the newly arrived Liquid Nitrogen Container right outside the glass chamber, Mahardika stated that the lab received generous contributions and assistance from several concerned individuals.

"That is one of the personal donations we have received. Help comes from many places, such as from my colleagues in the University of Hong Kong and Iowa State University," he said.

Worth around Rp 40 million (US$4,400), the container can maintain a steady internal temperature of -196? Celsius, which is ideal for storing virus samples.

So far, Mahardika and his dedicated staff of around 20 students and lecturers from the veterinary medicine department have collected and tested more than 8,000 samples.

Using the complicated yet accurate method of PCR (polymerase chain reaction), the students carefully render the virus powerless with a mixture of fenol, chloroform and alcohol.

They then isolate and extract the virus from the sample using the centrifuge machine, which rotates the sample at a speed of up to 11,300 revs per minute.

The RNA (Ribonucleic acid) thus extracted is then mixed with a buffer solution, several enzymes and genetic primers before being placed inside a thermocycler instrument, which repeatedly manipulates the temperature surrounding the mixture.

This process transforms the RNA into DNA (deoxyribonucleic acid) and also multiplies the amount of the DNA.

The final step, an electroforesis procedure conducted on the resulting DNA, will reveal the type and subtype of the virus.

"A more complete result, which includes the virus' genotype, could be achieved if we took another step, placing the DNA in the sequencing machine.

Unfortunately, we don't have the necessary machine yet. We must send the DNA sample to Jakarta for that step," he said.

Having his lab fitted with a Rp 2.5 billion (US$275,000) sequencing machine is Mahardika's current aim.

http://www.thejakartapost.com/detailfeatures.asp?fileid=20061102.T02&irec=1
 
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