tetano
Editor, Senior Moderator
Influenza Other Respi Viruses. 2013 Aug 21. doi: 10.1111/irv.12155. [Epub ahead of print]
Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection.
Giuffrida MJ, Valero N, Mosquera J, Alvarez de Mon M, Chac?n B, Espina LM, Gotera J, Bermudez J, Mavarez A.
Source
Facultad de Medicina, Instituto de Investigaciones Cl?nicas "Dr. Am?rico Negrette", Universidad del Zulia, Maracaibo, Venezuela.
Abstract
BACKGROUND:
Respiratory viral infections can induce different cytokine/chemokine profiles in lung tissues and have a significant influence on patients with asthma. There is little information about the systemic cytokine status in viral respiratory-infected asthmatic patients compared with non-asthmatic patients.
OBJECTIVES:
The aim of this study was to determine changes in circulating cytokines (IL-1β, TNF-α, IL-4, IL-5) and chemokines (MCP1: monocyte chemoattractant protein-1 and RANTES: regulated on activation normal T cell expressed and secreted) in patients with an asthmatic versus a non-asthmatic background with respiratory syncytial virus, parainfluenza virus or adenovirus respiratory infection. In addition, human monocyte cultures were incubated with respiratory viruses to determine the cytokine/chemokine profiles.
PATIENTS/METHODS:
Patients with asthmatic (n = 34) and non-asthmatic (n = 18) history and respiratory infections with respiratory syncytial virus, parainfluenza, and adenovirus were studied. Healthy individuals with similar age and sex (n = 10) were used as controls. Cytokine/chemokine content in blood and culture supernatants was determined by ELISA. Monocytes were isolated by Hystopaque gradient and cocultured with each of the above-mentioned viruses.
RESULTS:
Similar increased cytokine concentrations were observed in asthmatic and non-asthmatic patients. However, higher concentrations of chemokines were observed in asthmatic patients. Virus-infected monocyte cultures showed similar cytokine/chemokine profiles to those observed in the patients.
CONCLUSIONS:
Circulating cytokine profiles induced by acute viral lung infection were not related to asthmatic status, except for chemokines that were already increased in the asthmatic status. Monocytes could play an important role in the increased circulating concentration of cytokines found during respiratory viral infections.
? 2013 John Wiley & Sons Ltd.
KEYWORDS:
Asthma, chemokines, interleukins, viral lung infection
PMID:
23962134
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23962134
Increased cytokine/chemokines in serum from asthmatic and non-asthmatic patients with viral respiratory infection.
Giuffrida MJ, Valero N, Mosquera J, Alvarez de Mon M, Chac?n B, Espina LM, Gotera J, Bermudez J, Mavarez A.
Source
Facultad de Medicina, Instituto de Investigaciones Cl?nicas "Dr. Am?rico Negrette", Universidad del Zulia, Maracaibo, Venezuela.
Abstract
BACKGROUND:
Respiratory viral infections can induce different cytokine/chemokine profiles in lung tissues and have a significant influence on patients with asthma. There is little information about the systemic cytokine status in viral respiratory-infected asthmatic patients compared with non-asthmatic patients.
OBJECTIVES:
The aim of this study was to determine changes in circulating cytokines (IL-1β, TNF-α, IL-4, IL-5) and chemokines (MCP1: monocyte chemoattractant protein-1 and RANTES: regulated on activation normal T cell expressed and secreted) in patients with an asthmatic versus a non-asthmatic background with respiratory syncytial virus, parainfluenza virus or adenovirus respiratory infection. In addition, human monocyte cultures were incubated with respiratory viruses to determine the cytokine/chemokine profiles.
PATIENTS/METHODS:
Patients with asthmatic (n = 34) and non-asthmatic (n = 18) history and respiratory infections with respiratory syncytial virus, parainfluenza, and adenovirus were studied. Healthy individuals with similar age and sex (n = 10) were used as controls. Cytokine/chemokine content in blood and culture supernatants was determined by ELISA. Monocytes were isolated by Hystopaque gradient and cocultured with each of the above-mentioned viruses.
RESULTS:
Similar increased cytokine concentrations were observed in asthmatic and non-asthmatic patients. However, higher concentrations of chemokines were observed in asthmatic patients. Virus-infected monocyte cultures showed similar cytokine/chemokine profiles to those observed in the patients.
CONCLUSIONS:
Circulating cytokine profiles induced by acute viral lung infection were not related to asthmatic status, except for chemokines that were already increased in the asthmatic status. Monocytes could play an important role in the increased circulating concentration of cytokines found during respiratory viral infections.
? 2013 John Wiley & Sons Ltd.
KEYWORDS:
Asthma, chemokines, interleukins, viral lung infection
PMID:
23962134
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23962134