tetano
Editor, Senior Moderator
Vet Microbiol. 2014 May 13. pii: S0378-1135(14)00243-0. doi: 10.1016/j.vetmic.2014.05.007. [Epub ahead of print]
Inactivated vaccine with adjuvants consisting of pattern recognition receptor agonists confers protection against avian influenza viruses in chickens.
Tang Y1, Lu J2, Wu P2, Liu Z2, Tian Z2, Zha G2, Chen H2, Wang Q2, Wang Q3, Hou F2, Kang SM4, Hou J5.
Author information
Abstract
Use of adjuvant containing pathogen pattern recognition receptor agonists is one of the effective strategies to enhance the efficacy of licensed vaccines. In this study, we investigated the efficacy of avian influenza vaccines containing an adjuvant (CVCVA5) which was composed of polyriboinosinic polyribocytidylic, resiquimod, imiquimod, muramyl dipeptide and levomisole. Avian influenza vaccines adjuvanted with CVCVA5 were found to induce significantly higher titers of hemagglutiniton inhibition antibodies (P≤0.01) than those of commercial vaccines at 2-, 3- and 4-week post vaccination in both specific pathogen free (SPF) chickens and field application. Furthermore, virus shedding was reduced in SPF chickens immunized with H9-CVCVA5 vaccine after H9 subtype heterologous virus challenge. The ratios of both CD3+CD4+ and CD3+CD8+ lymphocytes were slowly elevated in chickens immunized with H9-CVCVA5 vaccine. Lymphocytes adoptive transfer study indicates that CD8+ T lymphocyte subpopulation might have contributed to improved protection against heterologous virus challenge. Results of this study suggest that the adjuvant CVCVA5 was capable of enhancing the potency of existing avian influenza vaccines by increasing humoral and cellular immune response.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Adjuvants; Agonist; Avian influenza virus; Inactivated vaccine; Pattern recognition receptor; Toll-like receptor
PMID:
24894132
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24894132
Inactivated vaccine with adjuvants consisting of pattern recognition receptor agonists confers protection against avian influenza viruses in chickens.
Tang Y1, Lu J2, Wu P2, Liu Z2, Tian Z2, Zha G2, Chen H2, Wang Q2, Wang Q3, Hou F2, Kang SM4, Hou J5.
Author information
Abstract
Use of adjuvant containing pathogen pattern recognition receptor agonists is one of the effective strategies to enhance the efficacy of licensed vaccines. In this study, we investigated the efficacy of avian influenza vaccines containing an adjuvant (CVCVA5) which was composed of polyriboinosinic polyribocytidylic, resiquimod, imiquimod, muramyl dipeptide and levomisole. Avian influenza vaccines adjuvanted with CVCVA5 were found to induce significantly higher titers of hemagglutiniton inhibition antibodies (P≤0.01) than those of commercial vaccines at 2-, 3- and 4-week post vaccination in both specific pathogen free (SPF) chickens and field application. Furthermore, virus shedding was reduced in SPF chickens immunized with H9-CVCVA5 vaccine after H9 subtype heterologous virus challenge. The ratios of both CD3+CD4+ and CD3+CD8+ lymphocytes were slowly elevated in chickens immunized with H9-CVCVA5 vaccine. Lymphocytes adoptive transfer study indicates that CD8+ T lymphocyte subpopulation might have contributed to improved protection against heterologous virus challenge. Results of this study suggest that the adjuvant CVCVA5 was capable of enhancing the potency of existing avian influenza vaccines by increasing humoral and cellular immune response.
Copyright ? 2014 Elsevier B.V. All rights reserved.
KEYWORDS:
Adjuvants; Agonist; Avian influenza virus; Inactivated vaccine; Pattern recognition receptor; Toll-like receptor
PMID:
24894132
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24894132