tetano
Editor, Senior Moderator
PLoS One. 2017 Apr 5;12(4):e0174523. doi: 10.1371/journal.pone.0174523. eCollection 2017.
[h=1]In vivo inhibition of influenza A virus replication by RNA interference targeting the PB2 subunit via intratracheal delivery.[/h] Huang DT[SUP]1,[/SUP][SUP]2[/SUP], Lu CY[SUP]3[/SUP], Shao PL[SUP]3[/SUP], Chang LY[SUP]2,[/SUP][SUP]3[/SUP], Wang JY[SUP]1[/SUP], Chang YH[SUP]3[/SUP], Lai MJ[SUP]3[/SUP], Chi YH[SUP]4[/SUP], Huang LM[SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza virus infection is a major threat to human health. Small interfering RNA (siRNA) is a promising approach for the prevention and treatment of viral infections. In this study, we constructed a series of DNA vector-based short hairpin RNAs (shRNAs) that target various genes of the influenza A virus using the polymerase III U6-RNA promoter to prevent influenza virus infection in vitro and in a mouse model.
[h=4]RESULTS:[/h] Three sets of DNA vector-based shRNA, two targeting genes encoding the polymerase acidic protein (PA) and one targeting polymerase basic protein 2 (PB2), efficiently inhibited the replication of influenza virus A/WSN/33(H1N1) in vitro. We also successfully prevented influenza virus A/WSN/33(H1N1) infection in a C57BL/6 mouse model by intratracheal delivery of anti-PB2 shRNA.
[h=4]CONCLUSIONS:[/h] Our findings suggest that the PB2-targeting shRNA plasmid showed potential for use as an RNAi-based therapeutic for influenza virus infection.
PMID: 28380007 DOI: 10.1371/journal.pone.0174523
[h=1]In vivo inhibition of influenza A virus replication by RNA interference targeting the PB2 subunit via intratracheal delivery.[/h] Huang DT[SUP]1,[/SUP][SUP]2[/SUP], Lu CY[SUP]3[/SUP], Shao PL[SUP]3[/SUP], Chang LY[SUP]2,[/SUP][SUP]3[/SUP], Wang JY[SUP]1[/SUP], Chang YH[SUP]3[/SUP], Lai MJ[SUP]3[/SUP], Chi YH[SUP]4[/SUP], Huang LM[SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza virus infection is a major threat to human health. Small interfering RNA (siRNA) is a promising approach for the prevention and treatment of viral infections. In this study, we constructed a series of DNA vector-based short hairpin RNAs (shRNAs) that target various genes of the influenza A virus using the polymerase III U6-RNA promoter to prevent influenza virus infection in vitro and in a mouse model.
[h=4]RESULTS:[/h] Three sets of DNA vector-based shRNA, two targeting genes encoding the polymerase acidic protein (PA) and one targeting polymerase basic protein 2 (PB2), efficiently inhibited the replication of influenza virus A/WSN/33(H1N1) in vitro. We also successfully prevented influenza virus A/WSN/33(H1N1) infection in a C57BL/6 mouse model by intratracheal delivery of anti-PB2 shRNA.
[h=4]CONCLUSIONS:[/h] Our findings suggest that the PB2-targeting shRNA plasmid showed potential for use as an RNAi-based therapeutic for influenza virus infection.
PMID: 28380007 DOI: 10.1371/journal.pone.0174523