tetano
Editor, Senior Moderator
Arch Med Res. 2014 Dec 15. pii: S0188-4409(14)00280-X. doi: 10.1016/j.arcmed.2014.12.002. [Epub ahead of print]
[h=1]In Vitro Evaluation of Synergistic Inhibitory Effects of Neuraminidase Inhibitors and Methylglyoxal Against Influenza Virus Infection.[/h] Charyasriwong S[SUP]1[/SUP], Watanabe K[SUP]1[/SUP], Rahmasari R[SUP]1[/SUP], Matsunaga A[SUP]1[/SUP], Haruyama T[SUP]2[/SUP], Kobayashi N[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND AND AIMS:[/h] Influenza virus infections are serious public health concerns worldwide that cause considerable mortality and morbidity. Moreover, the emergence of resistance to anti-influenza viral agents underscores the need to develop new anti-influenza viral agents and novel treatment strategies. Recently, we identified anti-influenza viral activity of manuka honey. Therefore, we hypothesized that methylglyoxal (MGO), a key component of manuka honey, may impart anti-influenza viral activity. The aim of this study was to evaluate the anti-influenza viral activity of MGO and its potential in combination treatments with neuraminidase (NA) inhibitors.
[h=4]METHODS:[/h] MDCK cells were used to evaluate anti-influenza viral activity. To evaluate the mechanism of MGO, plaque inhibition assays were performed. The synergistic effects of MGO and viral NA inhibitors were tested.
[h=4]RESULTS:[/h] MGO inhibited influenza virus A/WSN/33 replication 50% inhibitory concentration = 240 ? 190 μmol; 50% cytotoxic concentration = 1.4 ? 0.4 mmol; selective index (SI) = 5.8, which is related to its virucidal effects. Moreover, we found that MGO showed promising activity against various influenza strains. A synergistic effect was observed by a marked increase in SI of NA inhibitors at ∼1/100[SUP]th[/SUP] of their single usage. A synergistic effect of MGO and oseltamivir was also observed against oseltamivir-resistant virus.
[h=4]CONCLUSIONS:[/h] Our results showed that MGO has potent inhibitory activity against influenza viruses and also enhanced the effect of NA inhibitors. Thus, the co-administration of MGO and NA inhibitors should be considered for treatment of influenza virus infections.
Copyright ? 2014 IMSS. Published by Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Anti-influenza viral drug; Influenza virus; Manuka honey; Methylglyoxal; Neuraminidase inhibitors; Synergistic effect
PMID: 25523147 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25523147
[h=1]In Vitro Evaluation of Synergistic Inhibitory Effects of Neuraminidase Inhibitors and Methylglyoxal Against Influenza Virus Infection.[/h] Charyasriwong S[SUP]1[/SUP], Watanabe K[SUP]1[/SUP], Rahmasari R[SUP]1[/SUP], Matsunaga A[SUP]1[/SUP], Haruyama T[SUP]2[/SUP], Kobayashi N[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND AND AIMS:[/h] Influenza virus infections are serious public health concerns worldwide that cause considerable mortality and morbidity. Moreover, the emergence of resistance to anti-influenza viral agents underscores the need to develop new anti-influenza viral agents and novel treatment strategies. Recently, we identified anti-influenza viral activity of manuka honey. Therefore, we hypothesized that methylglyoxal (MGO), a key component of manuka honey, may impart anti-influenza viral activity. The aim of this study was to evaluate the anti-influenza viral activity of MGO and its potential in combination treatments with neuraminidase (NA) inhibitors.
[h=4]METHODS:[/h] MDCK cells were used to evaluate anti-influenza viral activity. To evaluate the mechanism of MGO, plaque inhibition assays were performed. The synergistic effects of MGO and viral NA inhibitors were tested.
[h=4]RESULTS:[/h] MGO inhibited influenza virus A/WSN/33 replication 50% inhibitory concentration = 240 ? 190 μmol; 50% cytotoxic concentration = 1.4 ? 0.4 mmol; selective index (SI) = 5.8, which is related to its virucidal effects. Moreover, we found that MGO showed promising activity against various influenza strains. A synergistic effect was observed by a marked increase in SI of NA inhibitors at ∼1/100[SUP]th[/SUP] of their single usage. A synergistic effect of MGO and oseltamivir was also observed against oseltamivir-resistant virus.
[h=4]CONCLUSIONS:[/h] Our results showed that MGO has potent inhibitory activity against influenza viruses and also enhanced the effect of NA inhibitors. Thus, the co-administration of MGO and NA inhibitors should be considered for treatment of influenza virus infections.
Copyright ? 2014 IMSS. Published by Elsevier Inc. All rights reserved.
[h=4]KEYWORDS:[/h] Anti-influenza viral drug; Influenza virus; Manuka honey; Methylglyoxal; Neuraminidase inhibitors; Synergistic effect
PMID: 25523147 [PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25523147