tetano
Editor, Senior Moderator
Phytomedicine. 2011 Sep 5. [Epub ahead of print]
In vitro anti-influenza virus activity of a cardiotonic glycoside from Adenium obesum (Forssk.).
Kiyohara H, Ichino C, Kawamura Y, Nagai T, Sato N, Yamada H, Salama MM, Abdel-Sattar E.
Source
Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan; Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan; Oriental Medicine Research Center, Kitasato University, Tokyo, Japan.
Abstract
Methanolic extracts of six Saudi plants were screened for their in vitro antiviral activity using influenza virus A/PR/8/34 (H1N1) and MDCK cells in an MTT assay. The results indicated that the extracts of Adeniumobesum and Tephorosianubica possessed antiviral activity (99.3 and 93.3% inhibition at the concentration of 10μg/ml, respectively). Based on these results A. obesum was selected for further study by applying bioactivity-guided fractionation to isolate its antiviral principle. The antiviral principle was isolated from the chloroform fraction through solvent fractionation, combined open liquid chromatography and HPLC. The isolated active compound A was identified as oleandrigenin-β-d-glucosyl (1→4)-β-d-digitalose, on the basis of its spectral analysis (MS, 1D and 2D NMR). The isolated glycoside showed reduction of virus titre by 69.3% inhibition at concentration of 1μg/ml (IC(50)=0.86μg/ml).
Copyright ? 2011 Elsevier GmbH. All rights reserved.
PMID:
21899996
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21899996
In vitro anti-influenza virus activity of a cardiotonic glycoside from Adenium obesum (Forssk.).
Kiyohara H, Ichino C, Kawamura Y, Nagai T, Sato N, Yamada H, Salama MM, Abdel-Sattar E.
Source
Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan; Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan; Oriental Medicine Research Center, Kitasato University, Tokyo, Japan.
Abstract
Methanolic extracts of six Saudi plants were screened for their in vitro antiviral activity using influenza virus A/PR/8/34 (H1N1) and MDCK cells in an MTT assay. The results indicated that the extracts of Adeniumobesum and Tephorosianubica possessed antiviral activity (99.3 and 93.3% inhibition at the concentration of 10μg/ml, respectively). Based on these results A. obesum was selected for further study by applying bioactivity-guided fractionation to isolate its antiviral principle. The antiviral principle was isolated from the chloroform fraction through solvent fractionation, combined open liquid chromatography and HPLC. The isolated active compound A was identified as oleandrigenin-β-d-glucosyl (1→4)-β-d-digitalose, on the basis of its spectral analysis (MS, 1D and 2D NMR). The isolated glycoside showed reduction of virus titre by 69.3% inhibition at concentration of 1μg/ml (IC(50)=0.86μg/ml).
Copyright ? 2011 Elsevier GmbH. All rights reserved.
PMID:
21899996
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/21899996