tetano
Editor, Senior Moderator
J Virol Methods. 2014 Sep 17. pii: S0166-0934(14)00348-6. doi: 10.1016/j.jviromet.2014.09.006. [Epub ahead of print]
Improved specificity and reduced subtype cross-reactivity for antibody detection by ELISA using globular head domain recombinant hemagglutinin.
Li ZN1, Carney PJ2, Lin SC3, Li J2, Chang JC2, Veguilla V2, Stevens J2, Miller JD2, Levine M2, Katz JM2, Hancock K2.
Author information
Abstract
The relative performance of ELISA using globular head domain (GH) and ectodomain hemagglutinins (HAs) as antigens to detect influenza A virus IgG antibody responses was assessed. Assay sensitivity and subtype cross-reactivity were evaluated using sera collected from recipients of monovalent H5N1 vaccine and A(H1N1)pdm09 virus-infected persons. Assay specificity was determined using collections of sera from either individuals unexposed to either H5N1 or A(H1N1)pdm09 viruses or exposed to H5N1 or A(H1N1)pdm09 viruses through vaccination or infection, respectively. ELISA using GH HA showed a similar degree of sensitivity, significantly higher specificity, and significantly lower subtype cross-reactivity compared to ELISA using ectodomain HA.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
ELISA; Ectodomain hemagglutinin; Globular head domain HA; Influenza
PMID:
25239367
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25239367
Improved specificity and reduced subtype cross-reactivity for antibody detection by ELISA using globular head domain recombinant hemagglutinin.
Li ZN1, Carney PJ2, Lin SC3, Li J2, Chang JC2, Veguilla V2, Stevens J2, Miller JD2, Levine M2, Katz JM2, Hancock K2.
Author information
Abstract
The relative performance of ELISA using globular head domain (GH) and ectodomain hemagglutinins (HAs) as antigens to detect influenza A virus IgG antibody responses was assessed. Assay sensitivity and subtype cross-reactivity were evaluated using sera collected from recipients of monovalent H5N1 vaccine and A(H1N1)pdm09 virus-infected persons. Assay specificity was determined using collections of sera from either individuals unexposed to either H5N1 or A(H1N1)pdm09 viruses or exposed to H5N1 or A(H1N1)pdm09 viruses through vaccination or infection, respectively. ELISA using GH HA showed a similar degree of sensitivity, significantly higher specificity, and significantly lower subtype cross-reactivity compared to ELISA using ectodomain HA.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
ELISA; Ectodomain hemagglutinin; Globular head domain HA; Influenza
PMID:
25239367
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25239367