tetano
Editor, Senior Moderator
Virology. 2017 Jan 28;504:25-35. doi: 10.1016/j.virol.2017.01.015. [Epub ahead of print]
[h=1]Impacts of different expressions of PA-X protein on 2009 pandemic H1N1 virus replication, pathogenicity and host immune responses.[/h] Lee J[SUP]1[/SUP], Yu H[SUP]2[/SUP], Li Y[SUP]1[/SUP], Ma J[SUP]1[/SUP], Lang Y[SUP]1[/SUP], Duff M[SUP]1[/SUP], Henningson J[SUP]1[/SUP], Liu Q[SUP]2[/SUP], Li Y[SUP]1[/SUP], Nagy A[SUP]1[/SUP], Bawa B[SUP]1[/SUP], Li Z[SUP]3[/SUP], Tong G[SUP]3[/SUP], Richt JA[SUP]1[/SUP], Ma W[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Although several studies have investigated the functions of influenza PA-X, the impact of different expressions of PA-X protein including full-length, truncated or PA-X deficient forms on virus replication, pathogenicity and host response remains unclear. Herein, we generated two mutated viruses expressing a full-length or deficient PA-X protein based on the A/California/04/2009 (H1N1) virus that expresses a truncated PA-X to understand three different expressions of PA-X protein on virus replication, pathogenicity and host immune responses. The results showed that expression of either full-length or truncated PA-X protein enhanced viral replication and pathogenicity as well as reduced host innate immune response in mice by host shutoff activity when compared to the virus expressing the deficient PA-X form. Furthermore, the full-length PA-X expression exhibited a greater effect on virus pathogenicity than the truncated PA-X form. Our results provide novel insights of PA-X on viral replication, pathogenicity and host immune responses.
Copyright ? 2017. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] Host immune response; Influenza A virus; PA-X; Virus replication
PMID: 28142079 DOI: 10.1016/j.virol.2017.01.015
[PubMed - as supplied by publisher]
[h=1]Impacts of different expressions of PA-X protein on 2009 pandemic H1N1 virus replication, pathogenicity and host immune responses.[/h] Lee J[SUP]1[/SUP], Yu H[SUP]2[/SUP], Li Y[SUP]1[/SUP], Ma J[SUP]1[/SUP], Lang Y[SUP]1[/SUP], Duff M[SUP]1[/SUP], Henningson J[SUP]1[/SUP], Liu Q[SUP]2[/SUP], Li Y[SUP]1[/SUP], Nagy A[SUP]1[/SUP], Bawa B[SUP]1[/SUP], Li Z[SUP]3[/SUP], Tong G[SUP]3[/SUP], Richt JA[SUP]1[/SUP], Ma W[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Although several studies have investigated the functions of influenza PA-X, the impact of different expressions of PA-X protein including full-length, truncated or PA-X deficient forms on virus replication, pathogenicity and host response remains unclear. Herein, we generated two mutated viruses expressing a full-length or deficient PA-X protein based on the A/California/04/2009 (H1N1) virus that expresses a truncated PA-X to understand three different expressions of PA-X protein on virus replication, pathogenicity and host immune responses. The results showed that expression of either full-length or truncated PA-X protein enhanced viral replication and pathogenicity as well as reduced host innate immune response in mice by host shutoff activity when compared to the virus expressing the deficient PA-X form. Furthermore, the full-length PA-X expression exhibited a greater effect on virus pathogenicity than the truncated PA-X form. Our results provide novel insights of PA-X on viral replication, pathogenicity and host immune responses.
Copyright ? 2017. Published by Elsevier Inc.
[h=4]KEYWORDS:[/h] Host immune response; Influenza A virus; PA-X; Virus replication
PMID: 28142079 DOI: 10.1016/j.virol.2017.01.015
[PubMed - as supplied by publisher]