tetano
Editor, Senior Moderator
J Clin Med. 2019 Nov 14;8(11). pii: E1974. doi: 10.3390/jcm8111974. [h=1]Impact of Pregnancy on Intra-Host Genetic Diversity of Influenza A Viruses in Hospitalised Women: A Retrospective Cohort Study.[/h]
Destras G[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Pichon M[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Simon B[SUP]2[/SUP], Valette M[SUP]2,[/SUP][SUP]3[/SUP], Escuret V[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Bolze PA[SUP]4[/SUP], Dubernard G[SUP]5[/SUP], Gaucherand P[SUP]6[/SUP], Lina B[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Josset L[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h] 1 Virpath, INSERM U1111, CNRS UMR5308, International Center for Infectiology Research, ENS Lyon, Claude Bernard Lyon 1 University, 69008 Lyon, France. 2 Virology Laboratory, Infectious Agents Institute, CBN, Groupement Hospitalier Nord, Hospices Civils de Lyon, 69004 Lyon, France. 3 Centre National des Virus des infections Respiratoires, Infectious Agents Institute, CBN, Groupement Hospitalier Nord, 69004 Lyon, France. 4 Service de Chirurgie Gyn?cologique et Oncologique-Obst?trique, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 69310 Pierre-B?nite, France. 5 Hospices Civils de Lyon, Service de Gyn?cologie et d'Obst?trique, H?pital de la Croix Rousse, 69004 Lyon, France. 6 Consultation Obst?trique, Groupement Hospitalier Est, Hospices Civils de Lyon, 69500 Bron, France.
[h=3]Abstract[/h] Characterising dynamics of Influenza A Viruses (IAV) within-host evolution is an active field of research which may lead to a better understanding of viral pathogenesis. Using a pregnant mouse model, a study has recently suggested that immune modulation during pregnancy could promote the emergence of IAV quasispecies with increased virulence. Herein, we assess the clinical relevance of these findings in humans. We studied IAV intra-host diversity (ihD) in pregnant (n = 36) and non-pregnant (n = 23) women hospitalized in Lyon for IAV infection (01/2015-05/2018). Whole IAV genomes present in nasopharyngeal samples were sequenced in duplicate to analyze reproducible intra-host single nucleotide variants (ihSNV). Counts, relative frequencies and locations of ihSNV were used as indicators of ihD. The median ihSNV/kb counts per segment were between 0 and 1.3. There was >81% ihSNV at relative frequencies between 1-5% for H1N1 and >51% for H3N2 IAV. No significant difference was noted between pregnant and non-pregnant women when considering all or only non-synonymous ihSNV. Seven convergent non-synonymous ihSNV were found; none were significantly associated with pregnancy. These results suggest that modulation of the immune system during pregnancy in humans does not impact IAV ihD, in contrast to mice.
[h=4]KEYWORDS:[/h] influenza; pregnancy; quasispecies; ultra-deep sequencing; viral diversity
PMID: 31739505 DOI: 10.3390/jcm8111974
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Destras G[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Pichon M[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Simon B[SUP]2[/SUP], Valette M[SUP]2,[/SUP][SUP]3[/SUP], Escuret V[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Bolze PA[SUP]4[/SUP], Dubernard G[SUP]5[/SUP], Gaucherand P[SUP]6[/SUP], Lina B[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Josset L[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP].
[h=3]Author information[/h] 1 Virpath, INSERM U1111, CNRS UMR5308, International Center for Infectiology Research, ENS Lyon, Claude Bernard Lyon 1 University, 69008 Lyon, France. 2 Virology Laboratory, Infectious Agents Institute, CBN, Groupement Hospitalier Nord, Hospices Civils de Lyon, 69004 Lyon, France. 3 Centre National des Virus des infections Respiratoires, Infectious Agents Institute, CBN, Groupement Hospitalier Nord, 69004 Lyon, France. 4 Service de Chirurgie Gyn?cologique et Oncologique-Obst?trique, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 69310 Pierre-B?nite, France. 5 Hospices Civils de Lyon, Service de Gyn?cologie et d'Obst?trique, H?pital de la Croix Rousse, 69004 Lyon, France. 6 Consultation Obst?trique, Groupement Hospitalier Est, Hospices Civils de Lyon, 69500 Bron, France.
[h=3]Abstract[/h] Characterising dynamics of Influenza A Viruses (IAV) within-host evolution is an active field of research which may lead to a better understanding of viral pathogenesis. Using a pregnant mouse model, a study has recently suggested that immune modulation during pregnancy could promote the emergence of IAV quasispecies with increased virulence. Herein, we assess the clinical relevance of these findings in humans. We studied IAV intra-host diversity (ihD) in pregnant (n = 36) and non-pregnant (n = 23) women hospitalized in Lyon for IAV infection (01/2015-05/2018). Whole IAV genomes present in nasopharyngeal samples were sequenced in duplicate to analyze reproducible intra-host single nucleotide variants (ihSNV). Counts, relative frequencies and locations of ihSNV were used as indicators of ihD. The median ihSNV/kb counts per segment were between 0 and 1.3. There was >81% ihSNV at relative frequencies between 1-5% for H1N1 and >51% for H3N2 IAV. No significant difference was noted between pregnant and non-pregnant women when considering all or only non-synonymous ihSNV. Seven convergent non-synonymous ihSNV were found; none were significantly associated with pregnancy. These results suggest that modulation of the immune system during pregnancy in humans does not impact IAV ihD, in contrast to mice.
[h=4]KEYWORDS:[/h] influenza; pregnancy; quasispecies; ultra-deep sequencing; viral diversity
PMID: 31739505 DOI: 10.3390/jcm8111974
Free full text