tetano
Editor, Senior Moderator
J Prev Med Hyg. 2018 Mar 30;59(1):E1-E7. doi: 10.15167/2421-4248/jpmh2018.59.1.870. eCollection 2018 Mar.
[h=1]Impact of erythrocyte species on assays for influenza serology.[/h] Trombetta CM[SUP]1[/SUP], Ulivieri C[SUP]2[/SUP], Cox RJ[SUP]3,[/SUP][SUP]4,[/SUP][SUP]5[/SUP], Remarque EJ[SUP]6[/SUP], Centi C[SUP]7[/SUP], Perini D[SUP]7[/SUP], Piccini G[SUP]7[/SUP], Rossi S[SUP]1[/SUP], Marchi S[SUP]1[/SUP], Montomoli E[SUP]1,[/SUP][SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza viruses have the ability to agglutinate erythrocytes by binding to sialic acid receptors on the host cell. Human influenza viruses preferentially bind to sialic acid linked to galactose by α 2.6 linkage, while avian influenza viruses preferentially bind to sialic acid linked to Gal by α 2.3 linkage. There is a close correlation between the ability of influenza A viruses to agglutinate erythrocytes from different animal species and their receptor specificity. The haemagglutination and haemagglutination inhibition assays are influenced by the species of erythrocytes. To provide an overview of the expression of sialic acid receptors on different erythrocytes, avian (turkey, chicken, pigeon) and mammalian (sheep, horse, human) species have been analysed by flow cytometry. Chicken, turkey and human erythrocytes display both types of linkages. Horse and sheep erythrocytes show almost exclusively α 2.3 Gal linkages, while pigeon erythrocytes express almost exclusively α 2.6 Gal linkages. The erythrocytes from the same avian and mammalian species have been evaluated by haemagglutination and haemagglutination inhibition assays with seasonal and avian strains. Chicken and turkey erythrocytes seem to be the most appropriate for both assays with seasonal influenza strains, in addition to pigeon erythrocytes, particularly for the B strains. In the case of the avian strain, chicken erythrocytes are suitable for haemagglutination assay and horse erythrocytes for haemagglutination inhibition assay. The choice of erythrocytes has a significant impact on the titres measured by both assays.
[h=4]KEYWORDS:[/h] Avian and mammalian erythrocytes; Haemagglutination assay; Haemagglutination inhibition assay; Sialic acid receptors
PMID: 29938233 PMCID: PMC6009068 DOI: 10.15167/2421-4248/jpmh2018.59.1.870
[h=1]Impact of erythrocyte species on assays for influenza serology.[/h] Trombetta CM[SUP]1[/SUP], Ulivieri C[SUP]2[/SUP], Cox RJ[SUP]3,[/SUP][SUP]4,[/SUP][SUP]5[/SUP], Remarque EJ[SUP]6[/SUP], Centi C[SUP]7[/SUP], Perini D[SUP]7[/SUP], Piccini G[SUP]7[/SUP], Rossi S[SUP]1[/SUP], Marchi S[SUP]1[/SUP], Montomoli E[SUP]1,[/SUP][SUP]7[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The influenza viruses have the ability to agglutinate erythrocytes by binding to sialic acid receptors on the host cell. Human influenza viruses preferentially bind to sialic acid linked to galactose by α 2.6 linkage, while avian influenza viruses preferentially bind to sialic acid linked to Gal by α 2.3 linkage. There is a close correlation between the ability of influenza A viruses to agglutinate erythrocytes from different animal species and their receptor specificity. The haemagglutination and haemagglutination inhibition assays are influenced by the species of erythrocytes. To provide an overview of the expression of sialic acid receptors on different erythrocytes, avian (turkey, chicken, pigeon) and mammalian (sheep, horse, human) species have been analysed by flow cytometry. Chicken, turkey and human erythrocytes display both types of linkages. Horse and sheep erythrocytes show almost exclusively α 2.3 Gal linkages, while pigeon erythrocytes express almost exclusively α 2.6 Gal linkages. The erythrocytes from the same avian and mammalian species have been evaluated by haemagglutination and haemagglutination inhibition assays with seasonal and avian strains. Chicken and turkey erythrocytes seem to be the most appropriate for both assays with seasonal influenza strains, in addition to pigeon erythrocytes, particularly for the B strains. In the case of the avian strain, chicken erythrocytes are suitable for haemagglutination assay and horse erythrocytes for haemagglutination inhibition assay. The choice of erythrocytes has a significant impact on the titres measured by both assays.
[h=4]KEYWORDS:[/h] Avian and mammalian erythrocytes; Haemagglutination assay; Haemagglutination inhibition assay; Sialic acid receptors
PMID: 29938233 PMCID: PMC6009068 DOI: 10.15167/2421-4248/jpmh2018.59.1.870