tetano
Editor, Senior Moderator
Influenza Other Respir Viruses. 2015 Nov 3. doi: 10.1111/irv.12356. [Epub ahead of print]
[h=1]Impact of a large deletion in the neuraminidase protein identified in a laninamivir-selected influenza A/Brisbane/10/2007 (H3N2) variant on viral fitness in vitro and in ferrets.[/h] Ann J[SUP]1[/SUP], Abed Y[SUP]1[/SUP], Beaulieu E[SUP]2[/SUP], Bouhy X[SUP]1[/SUP], Joly MH[SUP]2[/SUP], Dub? K[SUP]2[/SUP], Carbonneau J[SUP]1[/SUP], Hamelin ME[SUP]1[/SUP], Mallett C[SUP]2[/SUP], Boivin G[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Viral fitness of a laninamivir-selected influenza A/Brisbane/10/2007-like (H3N2) isolate (LRVp9) containing a 237-amino acid neuraminidase deletion and a P194L hemagglutinin mutation was evaluated in vitro and in ferrets. LRVp9 and the wild-type (WT) virus showed comparable replication kinetics in MDCK-ST6GalI cells. Cultured virus was recovered between days 2 and 5 post-infection in nasal washes (NW) from the 4 WT-infected ferrets whereas no virus was recovered from the LRVp9-infected animals. There was a ≥ 1 Log reduction in viral RNA copies/μl of NW for LRVp9 compared to WT at most time points. The large neuraminidase deletion compromises viral infectivity in vivo. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] A(H3N2); deletion; influenza; neuraminidase; resistance
PMID: 26526406 [PubMed - as supplied by publisher] Free full text
[h=1]Impact of a large deletion in the neuraminidase protein identified in a laninamivir-selected influenza A/Brisbane/10/2007 (H3N2) variant on viral fitness in vitro and in ferrets.[/h] Ann J[SUP]1[/SUP], Abed Y[SUP]1[/SUP], Beaulieu E[SUP]2[/SUP], Bouhy X[SUP]1[/SUP], Joly MH[SUP]2[/SUP], Dub? K[SUP]2[/SUP], Carbonneau J[SUP]1[/SUP], Hamelin ME[SUP]1[/SUP], Mallett C[SUP]2[/SUP], Boivin G[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Viral fitness of a laninamivir-selected influenza A/Brisbane/10/2007-like (H3N2) isolate (LRVp9) containing a 237-amino acid neuraminidase deletion and a P194L hemagglutinin mutation was evaluated in vitro and in ferrets. LRVp9 and the wild-type (WT) virus showed comparable replication kinetics in MDCK-ST6GalI cells. Cultured virus was recovered between days 2 and 5 post-infection in nasal washes (NW) from the 4 WT-infected ferrets whereas no virus was recovered from the LRVp9-infected animals. There was a ≥ 1 Log reduction in viral RNA copies/μl of NW for LRVp9 compared to WT at most time points. The large neuraminidase deletion compromises viral infectivity in vivo. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] A(H3N2); deletion; influenza; neuraminidase; resistance
PMID: 26526406 [PubMed - as supplied by publisher] Free full text