tetano
Editor, Senior Moderator
Comp Immunol Microbiol Infect Dis. 2019 Aug;65:76-80. doi: 10.1016/j.cimid.2019.05.004. Epub 2019 May 10.
[h=1]Immunological properties of the SLLTEVET epitope of Influenza A virus in multiple display on filamentous M13 phage.[/h] Lotfi Z[SUP]1[/SUP], Golchin M[SUP]2[/SUP], Khalili-Yazdi A[SUP]1[/SUP], Khalili M[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Small peptides require large carriers to stimulate the humoral immune system. The filamentous phages, such as M13, have been proposed as vectors for expressing and carrying these peptides on their capsid surface. M2e 2-9 (SLLTEVET) residues of the transmembrane protein M2 of Influenza A virus are conserved and considered as a suitable target for immunization against a wide range of Influenza A virus strains. Here, M2e (2-9) sequence of Influenza A virus was fused to the N-terminus of the major coat protein gpVIII of M13 phage and was used to immunize broiler chickens. The results showed that the SLLTEVET peptide on the surface of M13 phage was expressed, the hybrid phage was immunogenic and produced specific antibodies against M2e (2-9) in broiler chickens.
Copyright ? 2019. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Immunization; Influenza A virus; M13 phage; M2e (2-9)
PMID: 31300131 DOI: 10.1016/j.cimid.2019.05.004
[h=1]Immunological properties of the SLLTEVET epitope of Influenza A virus in multiple display on filamentous M13 phage.[/h] Lotfi Z[SUP]1[/SUP], Golchin M[SUP]2[/SUP], Khalili-Yazdi A[SUP]1[/SUP], Khalili M[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Small peptides require large carriers to stimulate the humoral immune system. The filamentous phages, such as M13, have been proposed as vectors for expressing and carrying these peptides on their capsid surface. M2e 2-9 (SLLTEVET) residues of the transmembrane protein M2 of Influenza A virus are conserved and considered as a suitable target for immunization against a wide range of Influenza A virus strains. Here, M2e (2-9) sequence of Influenza A virus was fused to the N-terminus of the major coat protein gpVIII of M13 phage and was used to immunize broiler chickens. The results showed that the SLLTEVET peptide on the surface of M13 phage was expressed, the hybrid phage was immunogenic and produced specific antibodies against M2e (2-9) in broiler chickens.
Copyright ? 2019. Published by Elsevier Ltd.
[h=4]KEYWORDS:[/h] Immunization; Influenza A virus; M13 phage; M2e (2-9)
PMID: 31300131 DOI: 10.1016/j.cimid.2019.05.004