tetano
Editor, Senior Moderator
Curr Microbiol. 2013 May 17. [Epub ahead of print]
Immunologic Mechanism of Patchouli Alcohol Anti-H1N1 Influenza Virus May Through Regulation of the RLH Signal Pathway In Vitro.
Wu XL, Ju DH, Chen J, Yu B, Liu KL, He JX, Dai CQ, Wu S, Chang Z, Wang YP, Chen XY.
Source
Department of Traditional Chinese Medicine, Medical College of Jinan University, Guangzhou, China.
Abstract
Patchouli alcohol (PA) is a kind of methanol extracted from traditional Chinese medicine Pogostemonis Herba. Our research aimed to observe the anti-influenza virus role of PA in vitro. 16HBE (human respiratory epithelial cell) was infected by H1N1 (A/FM1/1/47) to set the cell model. Then the 16HBE was co-cultivated with three kinds of immune cells: dendritic cells, macrophages, and monocytes, PA (the concentration is 10 μg/mL) was added as a treatment intervention for 24 h. The immune cells and the supernate were collected for RT-PCR and ELISA detection related to RLH (RIG-1-like helicases) pathway. Results showed that the IL-4 and IFN-γ in supernate were increased after H1N1 infection, and the PA treatment suppressed the expression of cytokines and the mRNA of RLH pathway. PA anti-influenza virus may through regulate the RLH singal pathway.
PMID:
23680974
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23680974
Immunologic Mechanism of Patchouli Alcohol Anti-H1N1 Influenza Virus May Through Regulation of the RLH Signal Pathway In Vitro.
Wu XL, Ju DH, Chen J, Yu B, Liu KL, He JX, Dai CQ, Wu S, Chang Z, Wang YP, Chen XY.
Source
Department of Traditional Chinese Medicine, Medical College of Jinan University, Guangzhou, China.
Abstract
Patchouli alcohol (PA) is a kind of methanol extracted from traditional Chinese medicine Pogostemonis Herba. Our research aimed to observe the anti-influenza virus role of PA in vitro. 16HBE (human respiratory epithelial cell) was infected by H1N1 (A/FM1/1/47) to set the cell model. Then the 16HBE was co-cultivated with three kinds of immune cells: dendritic cells, macrophages, and monocytes, PA (the concentration is 10 μg/mL) was added as a treatment intervention for 24 h. The immune cells and the supernate were collected for RT-PCR and ELISA detection related to RLH (RIG-1-like helicases) pathway. Results showed that the IL-4 and IFN-γ in supernate were increased after H1N1 infection, and the PA treatment suppressed the expression of cytokines and the mRNA of RLH pathway. PA anti-influenza virus may through regulate the RLH singal pathway.
PMID:
23680974
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23680974