tetano
Editor, Senior Moderator
Immunol Med
. 2022 Aug 24;1-14.
doi: 10.1080/25785826.2022.2111905. Online ahead of print.
Immune response induced in rodents by anti-CoVid19 plasmid DNA vaccine via pyro-drive jet injector inoculation
Tomoyuki Nishikawa[SUP] 1 [/SUP], Chin Yang Chang[SUP] 1 [/SUP], Jiayu A Tai[SUP] 1 [/SUP], Hiroki Hayashi[SUP] 2 [/SUP], Jiao Sun[SUP] 2 [/SUP], Shiho Torii[SUP] 3 [/SUP], Chikako Ono[SUP] 3 [/SUP], Yoshiharu Matsuura[SUP] 3 [/SUP], Ryoko Ide[SUP] 4 [/SUP], Junichi Mineno[SUP] 5 [/SUP], Miwa Sasai[SUP] 6 7 [/SUP], Masahiro Yamamoto[SUP] 6 7 [/SUP], Hironori Nakagami[SUP] 2 [/SUP], Kunihiko Yamashita[SUP] 1 8 [/SUP]
Affiliations
Abstract
There is an urgent need to stop the coronavirus disease 2019 (COVID-19) pandemic through the development of efficient and safe vaccination methods. Over the short term, plasmid DNA vaccines can be developed as they are molecularly stable, thus facilitating easy transport and storage. pVAX1-SARS-CoV2-co was designed for the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) S protein. The antibodies produced led to immunoreactions against the S protein, an anti-receptor-binding-domain, and a neutralizing action of the pVAX1-SARS-CoV2-co, as previously confirmed. To promote the efficacy of the pVAX1-SARS-CoV2-co vaccine a pyro-drive jet injector (PJI) was used. An intradermally adjusted PJI demonstrated that the pVAX1-SARS-CoV2-co vaccine injection caused a high production of anti-S protein antibodies, triggered immunoreactions, and neutralized the actions against SARS-CoV-2. A high-dose pVAX1-SARS-CoV2-co intradermal injection using PJI did not cause any serious disorders in the rat model. A viral challenge confirmed that intradermally immunized mice were potently protected from COVID-19. A pVAX1-SARS-CoV2-co intradermal injection using PJI is a safe and promising vaccination method for overcoming the COVID-19 pandemic.
Keywords: SARS-CoV2; plasmid DNA vaccines; pyro-drive jet injector; vaccine delivery systems.
. 2022 Aug 24;1-14.
doi: 10.1080/25785826.2022.2111905. Online ahead of print.
Immune response induced in rodents by anti-CoVid19 plasmid DNA vaccine via pyro-drive jet injector inoculation
Tomoyuki Nishikawa[SUP] 1 [/SUP], Chin Yang Chang[SUP] 1 [/SUP], Jiayu A Tai[SUP] 1 [/SUP], Hiroki Hayashi[SUP] 2 [/SUP], Jiao Sun[SUP] 2 [/SUP], Shiho Torii[SUP] 3 [/SUP], Chikako Ono[SUP] 3 [/SUP], Yoshiharu Matsuura[SUP] 3 [/SUP], Ryoko Ide[SUP] 4 [/SUP], Junichi Mineno[SUP] 5 [/SUP], Miwa Sasai[SUP] 6 7 [/SUP], Masahiro Yamamoto[SUP] 6 7 [/SUP], Hironori Nakagami[SUP] 2 [/SUP], Kunihiko Yamashita[SUP] 1 8 [/SUP]
Affiliations
- PMID: 36001011
- DOI: 10.1080/25785826.2022.2111905
Abstract
There is an urgent need to stop the coronavirus disease 2019 (COVID-19) pandemic through the development of efficient and safe vaccination methods. Over the short term, plasmid DNA vaccines can be developed as they are molecularly stable, thus facilitating easy transport and storage. pVAX1-SARS-CoV2-co was designed for the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) S protein. The antibodies produced led to immunoreactions against the S protein, an anti-receptor-binding-domain, and a neutralizing action of the pVAX1-SARS-CoV2-co, as previously confirmed. To promote the efficacy of the pVAX1-SARS-CoV2-co vaccine a pyro-drive jet injector (PJI) was used. An intradermally adjusted PJI demonstrated that the pVAX1-SARS-CoV2-co vaccine injection caused a high production of anti-S protein antibodies, triggered immunoreactions, and neutralized the actions against SARS-CoV-2. A high-dose pVAX1-SARS-CoV2-co intradermal injection using PJI did not cause any serious disorders in the rat model. A viral challenge confirmed that intradermally immunized mice were potently protected from COVID-19. A pVAX1-SARS-CoV2-co intradermal injection using PJI is a safe and promising vaccination method for overcoming the COVID-19 pandemic.
Keywords: SARS-CoV2; plasmid DNA vaccines; pyro-drive jet injector; vaccine delivery systems.