tetano
Editor, Senior Moderator
Transpl Infect Dis. 2015 Mar 28. doi: 10.1111/tid.12382. [Epub ahead of print]
[h=1]Immunogenicity of virosomal adjuvanted trivalent influenza vaccination in allogeneic stem cell transplant recipients.[/h] Ambati A[SUP]1[/SUP], Einarsdottir S, Magalhaes I, Poiret T, Bodenstein R, LeBlanc K, Brune M, Maeurer M, Ljungman P.
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza vaccination is generally recommended to hematopoietic stem cell transplant (HSCT) recipients. However, the seasonal subunit vaccination response is frequently sub-optimal and calls to examine alternate, more efficient vaccination systems. We compared the immunogenicity of an adjuvanted virosomal influenza and subunit vaccine in HSCT recipients.
[h=4]METHODS:[/h] The immunogenicity after a single dose (0.5 mL) of adjuvanted trivalent virosomal vaccination was evaluated in a study cohort of 21 HSCT recipients and compared to a control cohort of 30 HSCT recipients who received a single dose (0.5 mL) of non-adjuvanted seasonal trivalent subunit vaccination over 4 seasons from 2010-2014. Whole blood interferon gamma (IFNγ) release assays were tested, both before and 30 days after vaccination, in response to influenza pandemic (pdm) H1N1, H3N2, and B antigens. HLA-A*02 dextramers, to gauge for the absolute number of antigen-specific CD8+ T cells, and pdm 2009 hemagglutinin inhibition (HI) assays, to test for neutralizing antibodies, were used as immunological readouts.
[h=4]RESULTS:[/h] The pdm HI titers were poor in both cohorts with only 23% (5/21) after virosomal vaccination and 13.3% (4/30) in the seasonal vaccine cohort having protective titers (≥ 40). The delta change of IFNγ production in response to influenza pdm H1N1 (P = 0.005) and influenza B antigens (P = 0.01) were significantly elevated in blood from individuals who received the virosomal as compared to the seasonal vaccine. The IFNγ response to pdm H1N1 was stronger (P<0.001), as compared to seasonal vaccination, in patients vaccinated > 6 month post HSCT. We detected a significant increase in the frequency of matrix 1 (GILGFVTL) dextramer-specific CD8 T cells after the virosomal vaccine (P = 0.01). No differences were seen in the hemagglutinin-specific CD8 T cells between the 2 cohorts.
[h=4]CONCLUSION:[/h] Vaccination using a virosomal delivery system is beneficial in eliciting robust cellular immune responses to pdm H1N1 influenza in SCT recipients. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Transplantation; adjuvant; dextramers; inactivated; influenza vaccination; interferon-gamma; seasonal; subunit; virosomal
PMID: 25817044 [PubMed - as supplied by publisher]
[h=1]Immunogenicity of virosomal adjuvanted trivalent influenza vaccination in allogeneic stem cell transplant recipients.[/h] Ambati A[SUP]1[/SUP], Einarsdottir S, Magalhaes I, Poiret T, Bodenstein R, LeBlanc K, Brune M, Maeurer M, Ljungman P.
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza vaccination is generally recommended to hematopoietic stem cell transplant (HSCT) recipients. However, the seasonal subunit vaccination response is frequently sub-optimal and calls to examine alternate, more efficient vaccination systems. We compared the immunogenicity of an adjuvanted virosomal influenza and subunit vaccine in HSCT recipients.
[h=4]METHODS:[/h] The immunogenicity after a single dose (0.5 mL) of adjuvanted trivalent virosomal vaccination was evaluated in a study cohort of 21 HSCT recipients and compared to a control cohort of 30 HSCT recipients who received a single dose (0.5 mL) of non-adjuvanted seasonal trivalent subunit vaccination over 4 seasons from 2010-2014. Whole blood interferon gamma (IFNγ) release assays were tested, both before and 30 days after vaccination, in response to influenza pandemic (pdm) H1N1, H3N2, and B antigens. HLA-A*02 dextramers, to gauge for the absolute number of antigen-specific CD8+ T cells, and pdm 2009 hemagglutinin inhibition (HI) assays, to test for neutralizing antibodies, were used as immunological readouts.
[h=4]RESULTS:[/h] The pdm HI titers were poor in both cohorts with only 23% (5/21) after virosomal vaccination and 13.3% (4/30) in the seasonal vaccine cohort having protective titers (≥ 40). The delta change of IFNγ production in response to influenza pdm H1N1 (P = 0.005) and influenza B antigens (P = 0.01) were significantly elevated in blood from individuals who received the virosomal as compared to the seasonal vaccine. The IFNγ response to pdm H1N1 was stronger (P<0.001), as compared to seasonal vaccination, in patients vaccinated > 6 month post HSCT. We detected a significant increase in the frequency of matrix 1 (GILGFVTL) dextramer-specific CD8 T cells after the virosomal vaccine (P = 0.01). No differences were seen in the hemagglutinin-specific CD8 T cells between the 2 cohorts.
[h=4]CONCLUSION:[/h] Vaccination using a virosomal delivery system is beneficial in eliciting robust cellular immune responses to pdm H1N1 influenza in SCT recipients. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[h=4]KEYWORDS:[/h] Transplantation; adjuvant; dextramers; inactivated; influenza vaccination; interferon-gamma; seasonal; subunit; virosomal
PMID: 25817044 [PubMed - as supplied by publisher]