tetano
Editor, Senior Moderator
Microbiol Immunol. 2013 Jul 4. doi: 10.1111/1348-0421.12079. [Epub ahead of print]
Immunogenicity and safety of H1N1 influenza hemagglutinin protein expressed in a baculovirus system.
Na HN, Kim KH, Song MK, Park HL, Lee EY, Shim SH, Park S, Nam JH.
Source
Department of Biotechnology, The Catholic University of Korea, Bucheon, 420-743, Republic of Korea.
Abstract
Although most of the influenza vaccines are produced in egg, new types of vaccines must be developed. We compared the immunogenicity and safety of a baculovirus-expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated "HA-Bac-K") with those of a commercially available baculovirus-expressed HA (designated "HA-Bac-C") and an Escherichia coli-expressed HA (designated "HA-E.Coli-K"). HA-Bac-K effectively induced hemagglutination inhibition (HI) and neutralization (NT) antibodies in mouse and ferret models. The different immunogenicities observed may be attributable to the different expression systems and purification protocols used. Our work suggests that HA expressed in a baculovirus system is an effective and safe candidate influenza vaccine.
This article is protected by copyright. All rights reserved.
KEYWORDS:
hemagglutinin, influenzavirus, vaccine
PMID:
23822120
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23822120
Immunogenicity and safety of H1N1 influenza hemagglutinin protein expressed in a baculovirus system.
Na HN, Kim KH, Song MK, Park HL, Lee EY, Shim SH, Park S, Nam JH.
Source
Department of Biotechnology, The Catholic University of Korea, Bucheon, 420-743, Republic of Korea.
Abstract
Although most of the influenza vaccines are produced in egg, new types of vaccines must be developed. We compared the immunogenicity and safety of a baculovirus-expressed hemagglutinin (HA) of H1N1 influenza virus (Korea/01/2009; designated "HA-Bac-K") with those of a commercially available baculovirus-expressed HA (designated "HA-Bac-C") and an Escherichia coli-expressed HA (designated "HA-E.Coli-K"). HA-Bac-K effectively induced hemagglutination inhibition (HI) and neutralization (NT) antibodies in mouse and ferret models. The different immunogenicities observed may be attributable to the different expression systems and purification protocols used. Our work suggests that HA expressed in a baculovirus system is an effective and safe candidate influenza vaccine.
This article is protected by copyright. All rights reserved.
KEYWORDS:
hemagglutinin, influenzavirus, vaccine
PMID:
23822120
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23822120