Mary Wilson
Well-known member
Published:July 18, 2022
DOI:https://doi.org/10.1016/j.immuni.2022.07.005
Yu Gao [SUP]19 [/SUP]Curtis Cai [SUP]19 [/SUP]David Wullimann, Hans-Gustaf Ljunggren, Soo Aleman, Marcus Buggert [SUP]20 [/SUP]Show all authors
Highlights
Many immunocompromised patients mount suboptimal humoral immunity after SARS-CoV-2 mRNA vaccination. Here, we assessed the single-cell profile of SARS-CoV-2-specific T cells post-mRNA vaccination in healthy individuals and patients with various forms of immunodeficiencies. Impaired vaccine-induced cell-mediated immunity was observed in many immunocompromised patients, particularly in solid organ transplant and chronic lymphocytic leukemia patients. Notably, individuals with an inherited lack of mature B cells (X-linked agammaglobulinemia; XLA) displayed highly functional spike-specific T cell responses. Single-cell RNA-sequencing further revealed that mRNA vaccination induced a broad functional spectrum of spike-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells in healthy individuals and patients with XLA. These responses were founded on polyclonal repertoires of CD4[SUP]+[/SUP] T cells and robust expansions of oligoclonal effector memory CD45RA[SUP]+[/SUP] CD8[SUP]+[/SUP] T cells with stem-like characteristics. Collectively, our data provide the functional continuum of SARS-CoV-2-specific T cell responses post-mRNA vaccination, highlighting that cell-mediated immunity is of variable functional quality across immunodeficiency syndromes.
https://www.cell.com/immunity/pdf/S...m/retrieve/pii/S1074761322003387?showall=true
DOI:https://doi.org/10.1016/j.immuni.2022.07.005
Yu Gao [SUP]19 [/SUP]Curtis Cai [SUP]19 [/SUP]David Wullimann, Hans-Gustaf Ljunggren, Soo Aleman, Marcus Buggert [SUP]20 [/SUP]Show all authors
Highlights
- Single-cell map of SARS-CoV-2-specific T cell functions after mRNA vaccination
- Impaired T cell function in solid organ transplant and chronic leukemia patients
- Individuals with an inherited lack of mature B cells possess functional T cells
- Persisting memory CD8+ T cells display a stem-like effector memory CD45RA+ phenotype
Many immunocompromised patients mount suboptimal humoral immunity after SARS-CoV-2 mRNA vaccination. Here, we assessed the single-cell profile of SARS-CoV-2-specific T cells post-mRNA vaccination in healthy individuals and patients with various forms of immunodeficiencies. Impaired vaccine-induced cell-mediated immunity was observed in many immunocompromised patients, particularly in solid organ transplant and chronic lymphocytic leukemia patients. Notably, individuals with an inherited lack of mature B cells (X-linked agammaglobulinemia; XLA) displayed highly functional spike-specific T cell responses. Single-cell RNA-sequencing further revealed that mRNA vaccination induced a broad functional spectrum of spike-specific CD4[SUP]+[/SUP] and CD8[SUP]+[/SUP] T cells in healthy individuals and patients with XLA. These responses were founded on polyclonal repertoires of CD4[SUP]+[/SUP] T cells and robust expansions of oligoclonal effector memory CD45RA[SUP]+[/SUP] CD8[SUP]+[/SUP] T cells with stem-like characteristics. Collectively, our data provide the functional continuum of SARS-CoV-2-specific T cell responses post-mRNA vaccination, highlighting that cell-mediated immunity is of variable functional quality across immunodeficiency syndromes.
https://www.cell.com/immunity/pdf/S...m/retrieve/pii/S1074761322003387?showall=true