tetano
Editor, Senior Moderator
Immunity
. 2024 Jun 28:S1074-7613(24)00311-X.
doi: 10.1016/j.immuni.2024.06.005. Online ahead of print. Primary nasal influenza infection rewires tissue-scale memory response dynamics
Samuel W Kazer[SUP] 1 [/SUP], Colette Matysiak Match[SUP] 2 [/SUP], Erica M Langan[SUP] 3 [/SUP], Marie-Angèle Messou[SUP] 2 [/SUP], Thomas J LaSalle[SUP] 4 [/SUP], Elise O'Leary[SUP] 5 [/SUP], Jessica Marbourg[SUP] 6 [/SUP], Katherine Naughton[SUP] 7 [/SUP], Ulrich H von Andrian[SUP] 2 [/SUP], Jose Ordovas-Montanes[SUP] 8 [/SUP]
Affiliations
The nasal mucosa is often the initial site of respiratory viral infection, replication, and transmission. Understanding how infection shapes tissue-scale primary and memory responses is critical for designing mucosal therapeutics and vaccines. We generated a single-cell RNA-sequencing atlas of the murine nasal mucosa, sampling three regions during primary influenza infection and rechallenge. Compositional analysis revealed restricted infection to the respiratory mucosa with stepwise changes in immune and epithelial cell subsets and states. We identified and characterized a rare subset of Krt13+ nasal immune-interacting floor epithelial (KNIIFE) cells, which concurrently increased with tissue-resident memory T (T[SUB]RM[/SUB])-like cells. Proportionality analysis, cell-cell communication inference, and microscopy underscored the CXCL16-CXCR6 axis between KNIIFE and T[SUB]RM[/SUB] cells. Secondary influenza challenge induced accelerated and coordinated myeloid and lymphoid responses without epithelial proliferation. Together, this atlas serves as a reference for viral infection in the upper respiratory tract and highlights the efficacy of local coordinated memory responses.
Keywords: airway; epithelial cells; inflammation; influenza; memory; nasal mucosa; respiratory infection; scRNA-seq; single-cell; viral infection.
. 2024 Jun 28:S1074-7613(24)00311-X.
doi: 10.1016/j.immuni.2024.06.005. Online ahead of print. Primary nasal influenza infection rewires tissue-scale memory response dynamics
Samuel W Kazer[SUP] 1 [/SUP], Colette Matysiak Match[SUP] 2 [/SUP], Erica M Langan[SUP] 3 [/SUP], Marie-Angèle Messou[SUP] 2 [/SUP], Thomas J LaSalle[SUP] 4 [/SUP], Elise O'Leary[SUP] 5 [/SUP], Jessica Marbourg[SUP] 6 [/SUP], Katherine Naughton[SUP] 7 [/SUP], Ulrich H von Andrian[SUP] 2 [/SUP], Jose Ordovas-Montanes[SUP] 8 [/SUP]
Affiliations
- PMID: 38964332
- DOI: 10.1016/j.immuni.2024.06.005
The nasal mucosa is often the initial site of respiratory viral infection, replication, and transmission. Understanding how infection shapes tissue-scale primary and memory responses is critical for designing mucosal therapeutics and vaccines. We generated a single-cell RNA-sequencing atlas of the murine nasal mucosa, sampling three regions during primary influenza infection and rechallenge. Compositional analysis revealed restricted infection to the respiratory mucosa with stepwise changes in immune and epithelial cell subsets and states. We identified and characterized a rare subset of Krt13+ nasal immune-interacting floor epithelial (KNIIFE) cells, which concurrently increased with tissue-resident memory T (T[SUB]RM[/SUB])-like cells. Proportionality analysis, cell-cell communication inference, and microscopy underscored the CXCL16-CXCR6 axis between KNIIFE and T[SUB]RM[/SUB] cells. Secondary influenza challenge induced accelerated and coordinated myeloid and lymphoid responses without epithelial proliferation. Together, this atlas serves as a reference for viral infection in the upper respiratory tract and highlights the efficacy of local coordinated memory responses.
Keywords: airway; epithelial cells; inflammation; influenza; memory; nasal mucosa; respiratory infection; scRNA-seq; single-cell; viral infection.