tetano
Editor, Senior Moderator
Immunity
. 2020 Oct 14;S1074-7613(20)30445-3.
doi: 10.1016/j.immuni.2020.10.004. Online ahead of print.
Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity
Tyler J Ripperger[SUP] 1 [/SUP], Jennifer L Uhrlaub[SUP] 2 [/SUP], Makiko Watanabe[SUP] 2 [/SUP], Rachel Wong[SUP] 3 [/SUP], Yvonne Castaneda[SUP] 2 [/SUP], Hannah A Pizzato[SUP] 3 [/SUP], Mallory R Thompson[SUP] 4 [/SUP], Christine Bradshaw[SUP] 2 [/SUP], Craig C Weinkauf[SUP] 5 [/SUP], Christian Bime[SUP] 6 [/SUP], Heidi L Erickson[SUP] 6 [/SUP], Kenneth Knox[SUP] 7 [/SUP], Billie Bixby[SUP] 6 [/SUP], Sairam Parthasarathy[SUP] 6 [/SUP], Sachin Chaudhary[SUP] 6 [/SUP], Bhupinder Natt[SUP] 6 [/SUP], Elaine Cristan[SUP] 6 [/SUP], Tammer El Aini[SUP] 6 [/SUP], Franz Rischard[SUP] 6 [/SUP], Janet Campion[SUP] 6 [/SUP], Madhav Chopra[SUP] 6 [/SUP], Michael Insel[SUP] 6 [/SUP], Afshin Sam[SUP] 6 [/SUP], James L Knepler[SUP] 6 [/SUP], Andrew P Capaldi[SUP] 8 [/SUP], Catherine M Spier[SUP] 9 [/SUP], Michael D Dake[SUP] 10 [/SUP], Taylor Edwards[SUP] 11 [/SUP], Matthew E Kaplan[SUP] 12 [/SUP], Serena Jain Scott[SUP] 13 [/SUP], Cameron Hypes[SUP] 14 [/SUP], Jarrod Mosier[SUP] 14 [/SUP], David T Harris[SUP] 15 [/SUP], Bonnie J LaFleur[SUP] 16 [/SUP], Ryan Sprissler[SUP] 17 [/SUP], Janko Nikolich-Žugich[SUP] 18 [/SUP], Deepta Bhattacharya[SUP] 19 [/SUP]
Affiliations
Abstract
We conducted a serological study to define correlates of immunity against SARS-CoV-2. Compared to those with mild coronavirus disease 2019 (COVID-19) cases, individuals with severe disease exhibited elevated virus-neutralizing titers and antibodies against the nucleocapsid (N) and the receptor binding domain (RBD) of the spike protein. Age and sex played lesser roles. All cases, including asymptomatic individuals, seroconverted by 2 weeks after PCR confirmation. Spike RBD and S2 and neutralizing antibodies remained detectable through 5-7 months after onset, whereas α-N titers diminished. Testing 5,882 members of the local community revealed only 1 sample with seroreactivity to both RBD and S2 that lacked neutralizing antibodies. This fidelity could not be achieved with either RBD or S2 alone. Thus, inclusion of multiple independent assays improved the accuracy of antibody tests in low-seroprevalence communities and revealed differences in antibody kinetics depending on the antigen. We conclude that neutralizing antibodies are stably produced for at least 5-7 months after SARS-CoV-2 infection.
Keywords: COVID-19; S2 domain; SARS-CoV-2; antibodies; neutralization; nucleocapsid protein; orthogonal serological tests; receptor binding domain; serological test; serology; spike protein.
. 2020 Oct 14;S1074-7613(20)30445-3.
doi: 10.1016/j.immuni.2020.10.004. Online ahead of print.
Orthogonal SARS-CoV-2 Serological Assays Enable Surveillance of Low-Prevalence Communities and Reveal Durable Humoral Immunity
Tyler J Ripperger[SUP] 1 [/SUP], Jennifer L Uhrlaub[SUP] 2 [/SUP], Makiko Watanabe[SUP] 2 [/SUP], Rachel Wong[SUP] 3 [/SUP], Yvonne Castaneda[SUP] 2 [/SUP], Hannah A Pizzato[SUP] 3 [/SUP], Mallory R Thompson[SUP] 4 [/SUP], Christine Bradshaw[SUP] 2 [/SUP], Craig C Weinkauf[SUP] 5 [/SUP], Christian Bime[SUP] 6 [/SUP], Heidi L Erickson[SUP] 6 [/SUP], Kenneth Knox[SUP] 7 [/SUP], Billie Bixby[SUP] 6 [/SUP], Sairam Parthasarathy[SUP] 6 [/SUP], Sachin Chaudhary[SUP] 6 [/SUP], Bhupinder Natt[SUP] 6 [/SUP], Elaine Cristan[SUP] 6 [/SUP], Tammer El Aini[SUP] 6 [/SUP], Franz Rischard[SUP] 6 [/SUP], Janet Campion[SUP] 6 [/SUP], Madhav Chopra[SUP] 6 [/SUP], Michael Insel[SUP] 6 [/SUP], Afshin Sam[SUP] 6 [/SUP], James L Knepler[SUP] 6 [/SUP], Andrew P Capaldi[SUP] 8 [/SUP], Catherine M Spier[SUP] 9 [/SUP], Michael D Dake[SUP] 10 [/SUP], Taylor Edwards[SUP] 11 [/SUP], Matthew E Kaplan[SUP] 12 [/SUP], Serena Jain Scott[SUP] 13 [/SUP], Cameron Hypes[SUP] 14 [/SUP], Jarrod Mosier[SUP] 14 [/SUP], David T Harris[SUP] 15 [/SUP], Bonnie J LaFleur[SUP] 16 [/SUP], Ryan Sprissler[SUP] 17 [/SUP], Janko Nikolich-Žugich[SUP] 18 [/SUP], Deepta Bhattacharya[SUP] 19 [/SUP]
Affiliations
- PMID: 33129373
- DOI: 10.1016/j.immuni.2020.10.004
Abstract
We conducted a serological study to define correlates of immunity against SARS-CoV-2. Compared to those with mild coronavirus disease 2019 (COVID-19) cases, individuals with severe disease exhibited elevated virus-neutralizing titers and antibodies against the nucleocapsid (N) and the receptor binding domain (RBD) of the spike protein. Age and sex played lesser roles. All cases, including asymptomatic individuals, seroconverted by 2 weeks after PCR confirmation. Spike RBD and S2 and neutralizing antibodies remained detectable through 5-7 months after onset, whereas α-N titers diminished. Testing 5,882 members of the local community revealed only 1 sample with seroreactivity to both RBD and S2 that lacked neutralizing antibodies. This fidelity could not be achieved with either RBD or S2 alone. Thus, inclusion of multiple independent assays improved the accuracy of antibody tests in low-seroprevalence communities and revealed differences in antibody kinetics depending on the antigen. We conclude that neutralizing antibodies are stably produced for at least 5-7 months after SARS-CoV-2 infection.
Keywords: COVID-19; S2 domain; SARS-CoV-2; antibodies; neutralization; nucleocapsid protein; orthogonal serological tests; receptor binding domain; serological test; serology; spike protein.