tetano
Editor, Senior Moderator
We have previously reported an age-related impairment in the serum antibody response topandemic (p)2009 H1N1, measured by hemagglutination inhibition assay and ELISA. Thepresent study extends these observations and evaluates IgG subclass distribution in healthyindividuals of different ages vaccinated during the 2011-2012 season.
Results: The 2011-2012 vaccination season was characterized by a vaccine containing the pandemic(p)2009 H1N1 strain for the third consecutive year.
All of our subjects were previouslyimmunized, and therefore seroprotected at t0. Nevertheless, aging impaired the serumantibody response to H1N1, as antibody titers increased after vaccination in young and less inelderly individuals.
The peak of the response was at day 7 (t7), in contrast with what isusually seen at day 21-28, suggesting a memory response characterized by the induction of anIgG subclass with a shorter half-life. We hypothesized that the IgG3 response, with its muchshorter half-life, might be more represented.
Antibodies were predominantly of the IgG1subclass in both age groups, although a robust IgG3 response was also induced and accountedfor a significant proportion of the overall response. IgG2 and IgG4 antibodies were atindiscernible levels.
We showed a much higher percentage of IgG3 (40-50%) than previouslyin the literature (less than 10%). To explain if this was associated with a particular cytokineprofile, we measured H1N1-induced T cell cytokines in vitro and found that IgG3 levels werepositively correlated with TNF-alpha and IL-6.
Moreover, activation-induced cytidine deaminase(AID) mRNA expression, a predictive biomarker of optimal in vivo vaccine response, wasfound to significantly correlate with IgG3 and also with IgG1 similar to what we have shownpreviously for total IgG.
Conclusions: In the 2011-2012 season, the pandemic (p)2009 H1N1 strain was present in the vaccine forthe third consecutive year and therefore each individual was seroprotected at t0. The peak ofthe response was at t7, suggesting a memory response characterized by a robust induction ofIgG3, which was associated with TNF-alpha and IL-6 production.
Both IgG1 and IgG3 responseswere decreased by age. AID was confirmed to be a predictive biomarker of optimal vaccineresponses.
Author: Daniela FrascaAlain DiazMaria RomeroNicholas V MendezAna Marie LandinBonnie B Blomberg
Credits/Source: Immunity &Ageing 2013, 10:14
http://7thspace.com/headlines/43634...ng_the_2011_2012influenza_vaccine_season.html
Results: The 2011-2012 vaccination season was characterized by a vaccine containing the pandemic(p)2009 H1N1 strain for the third consecutive year.
All of our subjects were previouslyimmunized, and therefore seroprotected at t0. Nevertheless, aging impaired the serumantibody response to H1N1, as antibody titers increased after vaccination in young and less inelderly individuals.
The peak of the response was at day 7 (t7), in contrast with what isusually seen at day 21-28, suggesting a memory response characterized by the induction of anIgG subclass with a shorter half-life. We hypothesized that the IgG3 response, with its muchshorter half-life, might be more represented.
Antibodies were predominantly of the IgG1subclass in both age groups, although a robust IgG3 response was also induced and accountedfor a significant proportion of the overall response. IgG2 and IgG4 antibodies were atindiscernible levels.
We showed a much higher percentage of IgG3 (40-50%) than previouslyin the literature (less than 10%). To explain if this was associated with a particular cytokineprofile, we measured H1N1-induced T cell cytokines in vitro and found that IgG3 levels werepositively correlated with TNF-alpha and IL-6.
Moreover, activation-induced cytidine deaminase(AID) mRNA expression, a predictive biomarker of optimal in vivo vaccine response, wasfound to significantly correlate with IgG3 and also with IgG1 similar to what we have shownpreviously for total IgG.
Conclusions: In the 2011-2012 season, the pandemic (p)2009 H1N1 strain was present in the vaccine forthe third consecutive year and therefore each individual was seroprotected at t0. The peak ofthe response was at t7, suggesting a memory response characterized by a robust induction ofIgG3, which was associated with TNF-alpha and IL-6 production.
Both IgG1 and IgG3 responseswere decreased by age. AID was confirmed to be a predictive biomarker of optimal vaccineresponses.
Author: Daniela FrascaAlain DiazMaria RomeroNicholas V MendezAna Marie LandinBonnie B Blomberg
Credits/Source: Immunity &Ageing 2013, 10:14
http://7thspace.com/headlines/43634...ng_the_2011_2012influenza_vaccine_season.html