tetano
Editor, Senior Moderator
J Appl Microbiol. 2013 Aug 12. doi: 10.1111/jam.12325. [Epub ahead of print]
Immune Responses of Chickens Inoculated with Recombinant Lactobacillus Expressing the Hemagglutinin of the Avian Influenza Virus.
Wang Z, Yu Q, Fu J, Liang J, Yang Q.
Source
Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
Abstract
AIMS:
To develop a safe, effective and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI), we have successfully constructed a recombinant lactobacillus (LDL17-pH) that expresses the foreign HPAI protein, hemagglutinin 1 (HA1 ).
METHODS AND RESULTS:
The mucosal and systemic immune responses that are triggered by LDL17-pH following the oral administration to 10-day-old chickens were evaluated. The results showed that LDL17-pH could significantly increase the specific anti-HA1 IgA antibody level in the mucosa and the anti-HA1 IgG level in sera. Tissues were isolated from trachea and Peyer's Patches(PPs)and cecal tonsils of chickens, and gene expression was analyzed via real-time quantitative PCR.
CONCLUSIONS:
The results showed that LDL17-pH could significantly induce the specific anti-HA1 IgA antibody level in the trachea and intestine, and the specific anti-HA1 IgG antibody level in the serum (p < 0.05). Additionally, LDL17-pH was in the capacity to induce expression of cytokines IFN-γ, TLR-2 and AvBD-9 in the PPs and cecal tonsils. Most importantly, the chickens that were immunized with LDL17-pH were protected against lethal challenge of the H5N1 virus to some extent.
SIGNIFICANCE AND IMPACT OF STUDY:
Therefore, LDL17-pH could be a promising oral vaccine candidate against HPAI. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Lactobacillus, avian influenza virus, hemagglutinin, mucosal immune
PMID:
23937220
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23937220
Immune Responses of Chickens Inoculated with Recombinant Lactobacillus Expressing the Hemagglutinin of the Avian Influenza Virus.
Wang Z, Yu Q, Fu J, Liang J, Yang Q.
Source
Key Lab of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
Abstract
AIMS:
To develop a safe, effective and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI), we have successfully constructed a recombinant lactobacillus (LDL17-pH) that expresses the foreign HPAI protein, hemagglutinin 1 (HA1 ).
METHODS AND RESULTS:
The mucosal and systemic immune responses that are triggered by LDL17-pH following the oral administration to 10-day-old chickens were evaluated. The results showed that LDL17-pH could significantly increase the specific anti-HA1 IgA antibody level in the mucosa and the anti-HA1 IgG level in sera. Tissues were isolated from trachea and Peyer's Patches(PPs)and cecal tonsils of chickens, and gene expression was analyzed via real-time quantitative PCR.
CONCLUSIONS:
The results showed that LDL17-pH could significantly induce the specific anti-HA1 IgA antibody level in the trachea and intestine, and the specific anti-HA1 IgG antibody level in the serum (p < 0.05). Additionally, LDL17-pH was in the capacity to induce expression of cytokines IFN-γ, TLR-2 and AvBD-9 in the PPs and cecal tonsils. Most importantly, the chickens that were immunized with LDL17-pH were protected against lethal challenge of the H5N1 virus to some extent.
SIGNIFICANCE AND IMPACT OF STUDY:
Therefore, LDL17-pH could be a promising oral vaccine candidate against HPAI. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
Lactobacillus, avian influenza virus, hemagglutinin, mucosal immune
PMID:
23937220
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23937220