tetano
Editor, Senior Moderator
Microbiol Immunol. 2017 Apr 21. doi: 10.1111/1348-0421.12485. [Epub ahead of print]
[h=1]Immune response of highly conserved influenza A virus matrix 1 peptides.[/h] Lohia N[SUP]1[/SUP], Baranwal M[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza vaccine development is considered to be complicated and challenging. Constantly evolving influenza viruses require continuous global monitoring and reformulation of the vaccine strains. Peptide conserved among different strains and subtypes of influenza A virus are well-thought-out to be attractive targets for development of cross protective influenza vaccine based on cellular response. Three highly conserved (> 90%) matrix 1 peptides ILGFVFTLTVPSERGLQRRRF (P[SUB]M[/SUB] 1), LIRHENRMVLASTTAKA (P[SUB]M[/SUB] 2) and LQAYQKRMGVQMQR (P[SUB]M[/SUB] 3) containing multiple T cell epitopes have been assessed for their immunogenic potential in vitro, subjecting peripheral blood mononuclear cells from healthy volunteers to repetitive stimulation of these chemically synthesised peptides and measuring their interferon (IFN)-γ level and proliferation by ELISA (Enzyme-linked immunosorbent assay) and MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide) assay, respectively. Seven samples were screened for immunogenicity of P[SUB]M[/SUB] 1 and P[SUB]M[/SUB] 2, whereas six samples were tested for P[SUB]M[/SUB] 3. All six samples responded positive (IFN-γ secretion) to P[SUB]M[/SUB] 3 stimulation, followed by five and three for P[SUB]M[/SUB] 2 and P[SUB]M[/SUB] 1 respectively. Whereas, seven (P[SUB]M[/SUB] 1 and P[SUB]M[/SUB] 2) and four (P[SUB]M[/SUB] 3) samples have shown proliferative response as compared to unstimulated cells. Encouraging immunogenic response generated by these highly conserved matrix 1 peptides endorses their prospective candidature for broadly reactive influenza vaccine development.
? 2017 The Societies and Wiley Publishing Asia Pty Ltd.
[h=4]KEYWORDS:[/h] Hetero-subtypic; Interferon γ; Lymphocytes; Vaccine
PMID: 28429374 DOI: 10.1111/1348-0421.12485
[h=1]Immune response of highly conserved influenza A virus matrix 1 peptides.[/h] Lohia N[SUP]1[/SUP], Baranwal M[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Influenza vaccine development is considered to be complicated and challenging. Constantly evolving influenza viruses require continuous global monitoring and reformulation of the vaccine strains. Peptide conserved among different strains and subtypes of influenza A virus are well-thought-out to be attractive targets for development of cross protective influenza vaccine based on cellular response. Three highly conserved (> 90%) matrix 1 peptides ILGFVFTLTVPSERGLQRRRF (P[SUB]M[/SUB] 1), LIRHENRMVLASTTAKA (P[SUB]M[/SUB] 2) and LQAYQKRMGVQMQR (P[SUB]M[/SUB] 3) containing multiple T cell epitopes have been assessed for their immunogenic potential in vitro, subjecting peripheral blood mononuclear cells from healthy volunteers to repetitive stimulation of these chemically synthesised peptides and measuring their interferon (IFN)-γ level and proliferation by ELISA (Enzyme-linked immunosorbent assay) and MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide) assay, respectively. Seven samples were screened for immunogenicity of P[SUB]M[/SUB] 1 and P[SUB]M[/SUB] 2, whereas six samples were tested for P[SUB]M[/SUB] 3. All six samples responded positive (IFN-γ secretion) to P[SUB]M[/SUB] 3 stimulation, followed by five and three for P[SUB]M[/SUB] 2 and P[SUB]M[/SUB] 1 respectively. Whereas, seven (P[SUB]M[/SUB] 1 and P[SUB]M[/SUB] 2) and four (P[SUB]M[/SUB] 3) samples have shown proliferative response as compared to unstimulated cells. Encouraging immunogenic response generated by these highly conserved matrix 1 peptides endorses their prospective candidature for broadly reactive influenza vaccine development.
? 2017 The Societies and Wiley Publishing Asia Pty Ltd.
[h=4]KEYWORDS:[/h] Hetero-subtypic; Interferon γ; Lymphocytes; Vaccine
PMID: 28429374 DOI: 10.1111/1348-0421.12485