tetano
Editor, Senior Moderator
Int J Mol Sci. 2016 Dec 1;17(12). pii: E1990.
[h=1]Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.[/h] Chen S[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Wang A[SUP]4[/SUP], Sun L[SUP]5[/SUP], Liu F[SUP]6[/SUP], Wang M[SUP]7,[/SUP][SUP]8,[/SUP][SUP]9[/SUP], Jia R[SUP]10,[/SUP][SUP]11,[/SUP][SUP]12[/SUP], Zhu D[SUP]13,[/SUP][SUP]14[/SUP], Liu M[SUP]15,[/SUP][SUP]16,[/SUP][SUP]17[/SUP], Yang Q[SUP]18,[/SUP][SUP]19,[/SUP][SUP]20[/SUP], Wu Y[SUP]21,[/SUP][SUP]22,[/SUP][SUP]23[/SUP], Sun K[SUP]24,[/SUP][SUP]25,[/SUP][SUP]26[/SUP], Chen X[SUP]27,[/SUP][SUP]28[/SUP], Cheng A[SUP]29,[/SUP][SUP]30,[/SUP][SUP]31[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Goose parvovirus (GPV) and avian influenza virus subtype H9N2 are single-stranded DNA (ssDNA) and single-stranded RNA (ssRNA) viruses, respectively, both of which can spread in goslings and cause a significant economic loss. To explore the comprehensive transcriptome of GPV- or H9N2-infected goose spleens and to understand the immune responses induced by a DNA virus (GPV) or a RNA virus (H9N2), RNA-seq was performed on the spleens of goslings at the fifth day post infection. In the present study, 2604 and 2409 differentially expressed unigenes were identified in the GPV- and H9N2-infected groups, respectively. Through KEGG pathway enrichment analyses, the up-regulated transcripts in the two virus-infected groups were mainly involved in immune-related pathways. In addition, the two virus-infected groups displayed similar expression patterns in the immune response pathways, including pattern-recognition receptor signaling pathways, the antigen processing and presentation pathway, the NF-κB signaling pathway and the JAK-STAT signaling pathway, as well as cytokines. Furthermore, most of the immune-related genes, particularly TLR7, TRAF3, Mx, TRIM25, CD4, and CD8α, increased in response to GPV and H9N2 infection. However, the depression of NF-κB signaling may be a mechanism by which the viruses evade the host immune system or a strategy to achieve immune homeostasis.
[h=4]KEYWORDS:[/h] GPV; H9N2; identical expression profile; systemic transcriptome
PMID: 27916934 DOI: 10.3390/ijms17121990
[PubMed - in process]
[h=1]Immune-Related Gene Expression Patterns in GPV- or H9N2-Infected Goose Spleens.[/h] Chen S[SUP]1,[/SUP][SUP]2,[/SUP][SUP]3[/SUP], Wang A[SUP]4[/SUP], Sun L[SUP]5[/SUP], Liu F[SUP]6[/SUP], Wang M[SUP]7,[/SUP][SUP]8,[/SUP][SUP]9[/SUP], Jia R[SUP]10,[/SUP][SUP]11,[/SUP][SUP]12[/SUP], Zhu D[SUP]13,[/SUP][SUP]14[/SUP], Liu M[SUP]15,[/SUP][SUP]16,[/SUP][SUP]17[/SUP], Yang Q[SUP]18,[/SUP][SUP]19,[/SUP][SUP]20[/SUP], Wu Y[SUP]21,[/SUP][SUP]22,[/SUP][SUP]23[/SUP], Sun K[SUP]24,[/SUP][SUP]25,[/SUP][SUP]26[/SUP], Chen X[SUP]27,[/SUP][SUP]28[/SUP], Cheng A[SUP]29,[/SUP][SUP]30,[/SUP][SUP]31[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Goose parvovirus (GPV) and avian influenza virus subtype H9N2 are single-stranded DNA (ssDNA) and single-stranded RNA (ssRNA) viruses, respectively, both of which can spread in goslings and cause a significant economic loss. To explore the comprehensive transcriptome of GPV- or H9N2-infected goose spleens and to understand the immune responses induced by a DNA virus (GPV) or a RNA virus (H9N2), RNA-seq was performed on the spleens of goslings at the fifth day post infection. In the present study, 2604 and 2409 differentially expressed unigenes were identified in the GPV- and H9N2-infected groups, respectively. Through KEGG pathway enrichment analyses, the up-regulated transcripts in the two virus-infected groups were mainly involved in immune-related pathways. In addition, the two virus-infected groups displayed similar expression patterns in the immune response pathways, including pattern-recognition receptor signaling pathways, the antigen processing and presentation pathway, the NF-κB signaling pathway and the JAK-STAT signaling pathway, as well as cytokines. Furthermore, most of the immune-related genes, particularly TLR7, TRAF3, Mx, TRIM25, CD4, and CD8α, increased in response to GPV and H9N2 infection. However, the depression of NF-κB signaling may be a mechanism by which the viruses evade the host immune system or a strategy to achieve immune homeostasis.
[h=4]KEYWORDS:[/h] GPV; H9N2; identical expression profile; systemic transcriptome
PMID: 27916934 DOI: 10.3390/ijms17121990
[PubMed - in process]