tetano
Editor, Senior Moderator
Immune Netw
. 2023 Aug 4;23(4):e32.
doi: 10.4110/in.2023.23.e32. eCollection 2023 Aug. Vaccine Strategy That Enhances the Protective Efficacy of Systemic Immunization by Establishing Lung-Resident Memory CD8 T Cells Against Influenza Infection
Hyun-Jung Kong[SUP] 1 [/SUP], Youngwon Choi[SUP] 1 [/SUP], Eun-Ah Kim[SUP] 1 [/SUP], Jun Chang[SUP] 1 [/SUP]
Affiliations
Most influenza vaccines currently in use target the highly variable hemagglutinin protein to induce neutralizing antibodies and therefore require yearly reformulation. T cell-based universal influenza vaccines focus on eliciting broadly cross-reactive T-cell responses, especially the tissue-resident memory T cell (T[SUB]RM[/SUB]) population in the respiratory tract, providing superior protection to circulating memory T cells. This study demonstrated that intramuscular (i.m.) administration of the adenovirus-based vaccine expressing influenza virus nucleoprotein (rAd/NP) elicited weak CD8 T[SUB]RM[/SUB] responses in the lungs and airways, and yielded poor protection against lethal influenza virus challenge. However, a novel "prime-and-deploy" strategy that combines i.m. vaccination of rAd/NP with subsequent intranasal administration of an empty adenovector induced strong NP-specific CD8[SUP]+[/SUP] T[SUB]RM[/SUB] cells and provided complete protection against influenza virus challenge. Overall, our results demonstrate that this "prime-and-deploy" vaccination strategy is potentially applicable to the development of universal influenza vaccines.
Keywords: Adenovirus vector; Influenza vaccine; Intramuscular administration; Intranasal administration; Lung CD8 TRM.
. 2023 Aug 4;23(4):e32.
doi: 10.4110/in.2023.23.e32. eCollection 2023 Aug. Vaccine Strategy That Enhances the Protective Efficacy of Systemic Immunization by Establishing Lung-Resident Memory CD8 T Cells Against Influenza Infection
Hyun-Jung Kong[SUP] 1 [/SUP], Youngwon Choi[SUP] 1 [/SUP], Eun-Ah Kim[SUP] 1 [/SUP], Jun Chang[SUP] 1 [/SUP]
Affiliations
- PMID: 37670808
- PMCID: PMC10475829
- DOI: 10.4110/in.2023.23.e32
Most influenza vaccines currently in use target the highly variable hemagglutinin protein to induce neutralizing antibodies and therefore require yearly reformulation. T cell-based universal influenza vaccines focus on eliciting broadly cross-reactive T-cell responses, especially the tissue-resident memory T cell (T[SUB]RM[/SUB]) population in the respiratory tract, providing superior protection to circulating memory T cells. This study demonstrated that intramuscular (i.m.) administration of the adenovirus-based vaccine expressing influenza virus nucleoprotein (rAd/NP) elicited weak CD8 T[SUB]RM[/SUB] responses in the lungs and airways, and yielded poor protection against lethal influenza virus challenge. However, a novel "prime-and-deploy" strategy that combines i.m. vaccination of rAd/NP with subsequent intranasal administration of an empty adenovector induced strong NP-specific CD8[SUP]+[/SUP] T[SUB]RM[/SUB] cells and provided complete protection against influenza virus challenge. Overall, our results demonstrate that this "prime-and-deploy" vaccination strategy is potentially applicable to the development of universal influenza vaccines.
Keywords: Adenovirus vector; Influenza vaccine; Intramuscular administration; Intranasal administration; Lung CD8 TRM.