tetano
Editor, Senior Moderator
Immune Netw
. 2021 Aug 23;21(4):e28.
doi: 10.4110/in.2021.21.e28. eCollection 2021 Aug.
A "Prime and Deploy" Strategy for Universal Influenza Vaccine Targeting Nucleoprotein Induces Lung-Resident Memory CD8 T cells
Haerynn Chung[SUP] 1 [/SUP], Eun-Ah Kim[SUP] 1 [/SUP], Jun Chang[SUP] 1 [/SUP]
Affiliations
Abstract
Lung-resident memory T cells (T[SUB]RM[/SUB]) play an essential role in protecting against pulmonary virus infection. Parenteral administration of DNA vaccine is generally not sufficient to induce lung CD8 T[SUB]RM[/SUB] cells. This study investigates whether intramuscularly administered DNA vaccine expressing the nucleoprotein (NP) induces lung T[SUB]RM[/SUB] cells and protects against the influenza B virus. The results show that DNA vaccination poorly generates lung T[SUB]RM[/SUB] cells and massive secondary effector CD8 T cells entering the lungs after challenge infection do not offer sufficient protection. Nonetheless, intranasal administration of non-replicating adenovirus vector expressing no Ag following priming DNA vaccination deploys NP-specific CD8 T[SUB]RM[/SUB] cells in the lungs, which subsequently offers complete protection. This novel 'prime and deploy' strategy could be a promising regimen for a universal influenza vaccine targeting the conserved NP Ag.
Keywords: Adenovirus vector; DNA vaccine; Influenza B virus; Intranasal; Lung CD8 TRM.
. 2021 Aug 23;21(4):e28.
doi: 10.4110/in.2021.21.e28. eCollection 2021 Aug.
A "Prime and Deploy" Strategy for Universal Influenza Vaccine Targeting Nucleoprotein Induces Lung-Resident Memory CD8 T cells
Haerynn Chung[SUP] 1 [/SUP], Eun-Ah Kim[SUP] 1 [/SUP], Jun Chang[SUP] 1 [/SUP]
Affiliations
- PMID: 34522441
- PMCID: PMC8410988
- DOI: 10.4110/in.2021.21.e28
Abstract
Lung-resident memory T cells (T[SUB]RM[/SUB]) play an essential role in protecting against pulmonary virus infection. Parenteral administration of DNA vaccine is generally not sufficient to induce lung CD8 T[SUB]RM[/SUB] cells. This study investigates whether intramuscularly administered DNA vaccine expressing the nucleoprotein (NP) induces lung T[SUB]RM[/SUB] cells and protects against the influenza B virus. The results show that DNA vaccination poorly generates lung T[SUB]RM[/SUB] cells and massive secondary effector CD8 T cells entering the lungs after challenge infection do not offer sufficient protection. Nonetheless, intranasal administration of non-replicating adenovirus vector expressing no Ag following priming DNA vaccination deploys NP-specific CD8 T[SUB]RM[/SUB] cells in the lungs, which subsequently offers complete protection. This novel 'prime and deploy' strategy could be a promising regimen for a universal influenza vaccine targeting the conserved NP Ag.
Keywords: Adenovirus vector; DNA vaccine; Influenza B virus; Intranasal; Lung CD8 TRM.