tetano
Editor, Senior Moderator
Immun Inflamm Dis
. 2022 Apr;10(4):e595.
doi: 10.1002/iid3.595.
Long-term SARS-CoV-2-specific and cross-reactive cellular immune responses correlate with humoral responses, disease severity, and symptomatology
Ida Laurén[SUP] 1 [/SUP], Sebastian Havervall[SUP] 2 [/SUP], Henry Ng[SUP] 3 [/SUP], Martin Lord[SUP] 1 [/SUP], Aleksandra Pettke[SUP] 4 [/SUP], Nina Greilert-Norin[SUP] 2 [/SUP], Lena Gabrielsson[SUP] 2 [/SUP], Aikaterini Chourlia[SUP] 1 [/SUP], Catarina Amoêdo-Leite[SUP] 3 [/SUP], Vijay S Josyula[SUP] 3 [/SUP], Mohamed Eltahir[SUP] 1 5 [/SUP], Iliana Kerzeli[SUP] 1 [/SUP], August J Falk[SUP] 6 [/SUP], Jonathan Hober[SUP] 2 [/SUP], Wanda Christ[SUP] 7 [/SUP], Anna Wiberg[SUP] 5 [/SUP], My Hedhammar[SUP] 8 [/SUP], Hanna Tegel[SUP] 8 [/SUP], Joachim Burman[SUP] 9 [/SUP], Feifei Xu[SUP] 3 [/SUP], Elisa Pin[SUP] 6 [/SUP], Anna Månberg[SUP] 6 [/SUP], Jonas Klingström[SUP] 7 [/SUP], Gustaf Christoffersson[SUP] 3 [/SUP], Sophia Hober[SUP] 8 [/SUP], Peter Nilsson[SUP] 6 [/SUP], Mia Philipson[SUP] 3 [/SUP], Pierre Dönnes[SUP] 10 [/SUP], Robin Lindsay[SUP] 3 [/SUP], Charlotte Thålin[SUP] 2 [/SUP], Sara Mangsbo[SUP] 1 [/SUP]
Affiliations
Abstract
Background: Cellular immune memory responses post coronavirus disease 2019 (COVID-19) have been difficult to assess due to the risks of contaminating the immune response readout with memory responses stemming from previous exposure to endemic coronaviruses. The work herein presents a large-scale long-term follow-up study investigating the correlation between symptomology and cellular immune responses four to five months post seroconversion based on a unique severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific peptide pool that contains no overlapping peptides with endemic human coronaviruses.
Methods: Peptide stimulated memory T cell responses were assessed with dual interferon-gamma (IFNγ) and interleukin (IL)-2 Fluorospot. Serological analyses were performed using a multiplex antigen bead array.
Results: Our work demonstrates that long-term SARS-CoV-2-specific memory T cell responses feature dual IFNγ and IL-2 responses, whereas cross-reactive memory T cell responses primarily generate IFNγ in response to SARS-CoV-2 peptide stimulation. T cell responses correlated to long-term humoral immune responses. Disease severity as well as specific COVID-19 symptoms correlated with the magnitude of the SARS-CoV-2-specific memory T cell response four to five months post seroconversion.
Conclusion: Using a large cohort and a SARS-CoV-2-specific peptide pool we were able to substantiate that initial disease severity and symptoms correlate with the magnitude of the SARS-CoV-2-specific memory T cell responses.
Keywords: B-cell; IFNγ; IL-2; SARS-Cov-2; T cell.
. 2022 Apr;10(4):e595.
doi: 10.1002/iid3.595.
Long-term SARS-CoV-2-specific and cross-reactive cellular immune responses correlate with humoral responses, disease severity, and symptomatology
Ida Laurén[SUP] 1 [/SUP], Sebastian Havervall[SUP] 2 [/SUP], Henry Ng[SUP] 3 [/SUP], Martin Lord[SUP] 1 [/SUP], Aleksandra Pettke[SUP] 4 [/SUP], Nina Greilert-Norin[SUP] 2 [/SUP], Lena Gabrielsson[SUP] 2 [/SUP], Aikaterini Chourlia[SUP] 1 [/SUP], Catarina Amoêdo-Leite[SUP] 3 [/SUP], Vijay S Josyula[SUP] 3 [/SUP], Mohamed Eltahir[SUP] 1 5 [/SUP], Iliana Kerzeli[SUP] 1 [/SUP], August J Falk[SUP] 6 [/SUP], Jonathan Hober[SUP] 2 [/SUP], Wanda Christ[SUP] 7 [/SUP], Anna Wiberg[SUP] 5 [/SUP], My Hedhammar[SUP] 8 [/SUP], Hanna Tegel[SUP] 8 [/SUP], Joachim Burman[SUP] 9 [/SUP], Feifei Xu[SUP] 3 [/SUP], Elisa Pin[SUP] 6 [/SUP], Anna Månberg[SUP] 6 [/SUP], Jonas Klingström[SUP] 7 [/SUP], Gustaf Christoffersson[SUP] 3 [/SUP], Sophia Hober[SUP] 8 [/SUP], Peter Nilsson[SUP] 6 [/SUP], Mia Philipson[SUP] 3 [/SUP], Pierre Dönnes[SUP] 10 [/SUP], Robin Lindsay[SUP] 3 [/SUP], Charlotte Thålin[SUP] 2 [/SUP], Sara Mangsbo[SUP] 1 [/SUP]
Affiliations
- PMID: 35349756
- DOI: 10.1002/iid3.595
Abstract
Background: Cellular immune memory responses post coronavirus disease 2019 (COVID-19) have been difficult to assess due to the risks of contaminating the immune response readout with memory responses stemming from previous exposure to endemic coronaviruses. The work herein presents a large-scale long-term follow-up study investigating the correlation between symptomology and cellular immune responses four to five months post seroconversion based on a unique severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific peptide pool that contains no overlapping peptides with endemic human coronaviruses.
Methods: Peptide stimulated memory T cell responses were assessed with dual interferon-gamma (IFNγ) and interleukin (IL)-2 Fluorospot. Serological analyses were performed using a multiplex antigen bead array.
Results: Our work demonstrates that long-term SARS-CoV-2-specific memory T cell responses feature dual IFNγ and IL-2 responses, whereas cross-reactive memory T cell responses primarily generate IFNγ in response to SARS-CoV-2 peptide stimulation. T cell responses correlated to long-term humoral immune responses. Disease severity as well as specific COVID-19 symptoms correlated with the magnitude of the SARS-CoV-2-specific memory T cell response four to five months post seroconversion.
Conclusion: Using a large cohort and a SARS-CoV-2-specific peptide pool we were able to substantiate that initial disease severity and symptoms correlate with the magnitude of the SARS-CoV-2-specific memory T cell responses.
Keywords: B-cell; IFNγ; IL-2; SARS-Cov-2; T cell.