tetano
Editor, Senior Moderator
J Microbiol Biotechnol. 2012 May;22(5):699-707.
Identification of Reassortant Pandemic H1N1 Influenza Virus in Korean Pigs.
Han JY, Park SJ, Kim HK, Rho S, Nguyen GV, Song D, Kang BK, Moon HJ, Yeom MJ, Park BK.
Source
Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
Abstract
Since the 2009 pandemic human H1N1 influenza A virus emerged in April 2009, novel reassortant strains have been identified throughout the world. This paper describes the detection and isolation of reassortant strains associated with human pandemic influenza H1N1 and swine influenza H1N2 (SIV) viruses in swine populations in South Korea. Two influenza H1N2 reassortants were detected, and subtyped by PCR. The strains were isolated using Madin- Darby canine kidney (MDCK) cells, and genetically characterized by phylogenetic analysis for genetic diversity. They consisted of human, avian, and swine virus genes that were originated from the 2009 pandemic H1N1 virus and a neuraminidase (NA) gene from H1N2 SIV previously isolated in North America. This identification of reassortment events in swine farms raises concern that reassortant strains may continuously circulate within swine populations, calling for the further study and surveillance of pandemic H1N1 among swine.
PMID:
22561866
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22561866
Identification of Reassortant Pandemic H1N1 Influenza Virus in Korean Pigs.
Han JY, Park SJ, Kim HK, Rho S, Nguyen GV, Song D, Kang BK, Moon HJ, Yeom MJ, Park BK.
Source
Department of Veterinary Medicine Virology Laboratory College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea.
Abstract
Since the 2009 pandemic human H1N1 influenza A virus emerged in April 2009, novel reassortant strains have been identified throughout the world. This paper describes the detection and isolation of reassortant strains associated with human pandemic influenza H1N1 and swine influenza H1N2 (SIV) viruses in swine populations in South Korea. Two influenza H1N2 reassortants were detected, and subtyped by PCR. The strains were isolated using Madin- Darby canine kidney (MDCK) cells, and genetically characterized by phylogenetic analysis for genetic diversity. They consisted of human, avian, and swine virus genes that were originated from the 2009 pandemic H1N1 virus and a neuraminidase (NA) gene from H1N2 SIV previously isolated in North America. This identification of reassortment events in swine farms raises concern that reassortant strains may continuously circulate within swine populations, calling for the further study and surveillance of pandemic H1N1 among swine.
PMID:
22561866
[PubMed - in process]
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22561866