tetano
Editor, Senior Moderator
Int J Med Sci. 2016 Jan 25;13(1):77-84. doi: 10.7150/ijms.13301. eCollection 2016.
[h=1]Identification of microRNAs in Throat Swab as the Biomarkers for Diagnosis of Influenza.[/h] Peng F[SUP]1[/SUP], He J[SUP]2[/SUP], Loo JF[SUP]3[/SUP], Yao J[SUP]4[/SUP], Shi L[SUP]2[/SUP], Liu C[SUP]2[/SUP], Zhao C[SUP]2[/SUP], Xie W[SUP]5[/SUP], Shao Y[SUP]6[/SUP], Kong SK[SUP]3[/SUP], Gu D[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza is a serious worldwide disease that captures global attention in the past few years after outbreaks. The recent discoveries of microRNA (miRNA) and its unique expression profile in influenza patients have offered a new method for early influenza diagnosis. The aim of this study was to examine the utility of miRNAs for the diagnosis of influenza.
[h=4]METHODS:[/h] Thirteen selected miRNAs were investigated with the hosts' throat swabs (25 H1N1, 20 H3N2, 20 influenza B and 21 healthy controls) by real-time quantitative polymerase chain reaction (RT-qPCR) using U6 snRNA as endogenous control for normalization, and receiver operating characteristic (ROC) curve/Area under curve (AUC) for analysis.
[h=4]RESULTS:[/h] miR-29a-3p, miR-30c-5p, miR-34c-3p and miR-181a-5p are useful biomarkers for influenza A detection; and miR-30c-5p, miR-34b-5p, miR-205-5p and miR-449b-5p for influenza B detection. Also, use of both miR-30c-5p and miR-34c-3p (AUC=0.879); and miR-30c-5p and miR-449b-5p (AUC=0.901) are better than using one miRNA to confirm influenza A and influenza B infection, respectively.
[h=4]CONCLUSIONS:[/h] Given its simplicity, non-invasiveness and specificity, we found that the throat swab-derived miRNAs miR-29a-3p, miR-30c-5p, miR-34b-5p, miR-34c-3p, miR-181a-5p, miR-205-5p and miR-449b-5p are a useful tool for influenza diagnosis on influenza A and B.
[h=4]KEYWORDS:[/h] RT-qPCR; biomarkers.; influenza; miRNA; throat swab
PMID: 26917988 [PubMed - in process] PMCID: PMC4747873 Free PMC Article
[h=1]Identification of microRNAs in Throat Swab as the Biomarkers for Diagnosis of Influenza.[/h] Peng F[SUP]1[/SUP], He J[SUP]2[/SUP], Loo JF[SUP]3[/SUP], Yao J[SUP]4[/SUP], Shi L[SUP]2[/SUP], Liu C[SUP]2[/SUP], Zhao C[SUP]2[/SUP], Xie W[SUP]5[/SUP], Shao Y[SUP]6[/SUP], Kong SK[SUP]3[/SUP], Gu D[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] [h=4]BACKGROUND:[/h] Influenza is a serious worldwide disease that captures global attention in the past few years after outbreaks. The recent discoveries of microRNA (miRNA) and its unique expression profile in influenza patients have offered a new method for early influenza diagnosis. The aim of this study was to examine the utility of miRNAs for the diagnosis of influenza.
[h=4]METHODS:[/h] Thirteen selected miRNAs were investigated with the hosts' throat swabs (25 H1N1, 20 H3N2, 20 influenza B and 21 healthy controls) by real-time quantitative polymerase chain reaction (RT-qPCR) using U6 snRNA as endogenous control for normalization, and receiver operating characteristic (ROC) curve/Area under curve (AUC) for analysis.
[h=4]RESULTS:[/h] miR-29a-3p, miR-30c-5p, miR-34c-3p and miR-181a-5p are useful biomarkers for influenza A detection; and miR-30c-5p, miR-34b-5p, miR-205-5p and miR-449b-5p for influenza B detection. Also, use of both miR-30c-5p and miR-34c-3p (AUC=0.879); and miR-30c-5p and miR-449b-5p (AUC=0.901) are better than using one miRNA to confirm influenza A and influenza B infection, respectively.
[h=4]CONCLUSIONS:[/h] Given its simplicity, non-invasiveness and specificity, we found that the throat swab-derived miRNAs miR-29a-3p, miR-30c-5p, miR-34b-5p, miR-34c-3p, miR-181a-5p, miR-205-5p and miR-449b-5p are a useful tool for influenza diagnosis on influenza A and B.
[h=4]KEYWORDS:[/h] RT-qPCR; biomarkers.; influenza; miRNA; throat swab
PMID: 26917988 [PubMed - in process] PMCID: PMC4747873 Free PMC Article