tetano
Editor, Senior Moderator
J Gen Virol. 2015 Dec 16. doi: 10.1099/jgv.0.000358. [Epub ahead of print]
[h=1]Identification of cis-acting packaging signals in the coding regions of the influenza B virus HA gene segment.[/h] Sherry L[SUP]1[/SUP], Punovuori K[SUP]2[/SUP], Wallace LE[SUP]3[/SUP], Prangley E[SUP]4[/SUP], DeFries S[SUP]5[/SUP], Jackson D[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] For influenza A and B viruses to be infectious they require eight viral RNA (vRNA) genome segments to be packaged into virions. For efficient packaging influenza A viruses utilise cis-acting vRNA sequences, containing both non-coding and protein coding regions of each segment. Whether influenza B viruses have similar packaging signals is unknown. Here we show that coding regions at the 3' and 5' ends of the influenza B virus vRNA segment four are required for genome packaging, with the first 30 nucleotides at each end essential for this process. Synonymous mutation of these regions led to virus attenuation, an increase in defective particle production and a reduction in packaging of multiple vRNAs. Overall our data suggest that the influenza B virus vRNA gene segments likely interact with each other during the packaging process, which is driven by ?cis-acting packaging signals that extend into protein coding regions of the vRNA.
PMID: 26675486 [PubMed - as supplied by publisher]
[h=1]Identification of cis-acting packaging signals in the coding regions of the influenza B virus HA gene segment.[/h] Sherry L[SUP]1[/SUP], Punovuori K[SUP]2[/SUP], Wallace LE[SUP]3[/SUP], Prangley E[SUP]4[/SUP], DeFries S[SUP]5[/SUP], Jackson D[SUP]6[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] For influenza A and B viruses to be infectious they require eight viral RNA (vRNA) genome segments to be packaged into virions. For efficient packaging influenza A viruses utilise cis-acting vRNA sequences, containing both non-coding and protein coding regions of each segment. Whether influenza B viruses have similar packaging signals is unknown. Here we show that coding regions at the 3' and 5' ends of the influenza B virus vRNA segment four are required for genome packaging, with the first 30 nucleotides at each end essential for this process. Synonymous mutation of these regions led to virus attenuation, an increase in defective particle production and a reduction in packaging of multiple vRNAs. Overall our data suggest that the influenza B virus vRNA gene segments likely interact with each other during the packaging process, which is driven by ?cis-acting packaging signals that extend into protein coding regions of the vRNA.
PMID: 26675486 [PubMed - as supplied by publisher]