tetano
Editor, Senior Moderator
Cell Mol Immunol. 2016 May 9. doi: 10.1038/cmi.2016.20. [Epub ahead of print]
[h=1]Identification of a CD4 T-cell epitope in the hemagglutinin stalk domain of pandemic H1N1 influenza virus and its antigen-driven TCR usage signature in BALB/c mice.[/h] Lu IN[SUP]1[/SUP], Farinelle S[SUP]1[/SUP], Sausy A[SUP]1[/SUP], Muller CP[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The stalk region of the influenza virus hemagglutinin is relatively well conserved compared with the globular head domain, which makes it a potential target for use as a universal vaccine against influenza. However, the role of CD4 T cells in the hemagglutinin stalk-specific immune response is not clear. Here we identified a mouse CD4 T-cell epitope that encompasses residues HA2[SUB]113-131[/SUB] from the hemagglutinin stalk domain after a sub-lethal infection of influenza. In response to stimulation with the identified epitope, splenocytes derived from the infected mice showed significant polyfunctionality as shown by IL-2, TNF-α and IFN-γ production as well as degranulation. Moreover, mice immunized with the peptide corresponding to this CD4 T-cell epitope exhibited interindividual sharing of the CD4 T-cell receptor β sequences, and they had a higher survival rate following a challenge with a lethal dose of pandemic H1N1 influenza virus. Thus, our data demonstrated a crucial role of hemagglutinin stalk-specific CD4 T cells in the host immune response against influenza virus infection.Cellular & Molecular Immunology advance online publication, 9 May 2016; doi:10.1038/cmi.2016.20.
PMID: 27157498 [PubMed - as supplied by publisher]
[h=1]Identification of a CD4 T-cell epitope in the hemagglutinin stalk domain of pandemic H1N1 influenza virus and its antigen-driven TCR usage signature in BALB/c mice.[/h] Lu IN[SUP]1[/SUP], Farinelle S[SUP]1[/SUP], Sausy A[SUP]1[/SUP], Muller CP[SUP]1[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The stalk region of the influenza virus hemagglutinin is relatively well conserved compared with the globular head domain, which makes it a potential target for use as a universal vaccine against influenza. However, the role of CD4 T cells in the hemagglutinin stalk-specific immune response is not clear. Here we identified a mouse CD4 T-cell epitope that encompasses residues HA2[SUB]113-131[/SUB] from the hemagglutinin stalk domain after a sub-lethal infection of influenza. In response to stimulation with the identified epitope, splenocytes derived from the infected mice showed significant polyfunctionality as shown by IL-2, TNF-α and IFN-γ production as well as degranulation. Moreover, mice immunized with the peptide corresponding to this CD4 T-cell epitope exhibited interindividual sharing of the CD4 T-cell receptor β sequences, and they had a higher survival rate following a challenge with a lethal dose of pandemic H1N1 influenza virus. Thus, our data demonstrated a crucial role of hemagglutinin stalk-specific CD4 T cells in the host immune response against influenza virus infection.Cellular & Molecular Immunology advance online publication, 9 May 2016; doi:10.1038/cmi.2016.20.
PMID: 27157498 [PubMed - as supplied by publisher]