tetano
Editor, Senior Moderator
J Virol Methods. 2014 Jul 12. pii: S0166-0934(14)00259-6. doi: 10.1016/j.jviromet.2014.07.003. [Epub ahead of print]
Hydrogen peroxide inactivation of influenza virus preserves antigenic structure and immunogenicity.
Dembinski JL1, Hungnes O2, Hauge AG3, Kristoffersen AC4, Haneberg B4, Mjaaland S4.
Author information
Abstract
The use of live virus in the laboratory requires additional precautions, such as personnel training and special equipment, in order to limit the transmission risk. This is a requirement which not all laboratories can fulfill. In this study, a viral inactivation method is introduced using hydrogen peroxide (H2O2), which maintains antigenicity. Three strains of influenza viruses were inactivated and the ex vivo cellular and humoral immune responses were further analyzed, by comparing them to live viruses, in ELISpot, Multiplex and ELISA assays. In all assays, the H2O2 inactivated viruses displayed comparable responses to the live viruses, suggesting that the inactivated viruses still elicited immunogenic responses even though inactivation was confirmed by lack of viral replication in MDCK cells. Taken together, this study demonstrates that influenza viruses inactivated with H2O2 retain immunogenicity and are able to both detect humoral and elicit cellular immune responses in vitro, which could reduce the need to handle live viruses in the laboratory.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Hydrogen peroxide; Immune response; Immunogenic; Inactivation; Influenza virus
PMID:
25025814
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25025814
Hydrogen peroxide inactivation of influenza virus preserves antigenic structure and immunogenicity.
Dembinski JL1, Hungnes O2, Hauge AG3, Kristoffersen AC4, Haneberg B4, Mjaaland S4.
Author information
Abstract
The use of live virus in the laboratory requires additional precautions, such as personnel training and special equipment, in order to limit the transmission risk. This is a requirement which not all laboratories can fulfill. In this study, a viral inactivation method is introduced using hydrogen peroxide (H2O2), which maintains antigenicity. Three strains of influenza viruses were inactivated and the ex vivo cellular and humoral immune responses were further analyzed, by comparing them to live viruses, in ELISpot, Multiplex and ELISA assays. In all assays, the H2O2 inactivated viruses displayed comparable responses to the live viruses, suggesting that the inactivated viruses still elicited immunogenic responses even though inactivation was confirmed by lack of viral replication in MDCK cells. Taken together, this study demonstrates that influenza viruses inactivated with H2O2 retain immunogenicity and are able to both detect humoral and elicit cellular immune responses in vitro, which could reduce the need to handle live viruses in the laboratory.
Copyright ? 2014. Published by Elsevier B.V.
KEYWORDS:
Hydrogen peroxide; Immune response; Immunogenic; Inactivation; Influenza virus
PMID:
25025814
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25025814