tetano
Editor, Senior Moderator
Hum Vaccin Immunother. 2015 May 21:0. [Epub ahead of print]
[h=1]Humoral and cellular immune responses after influenza vaccination in patients with postcancer fatigue.[/h] Prinsen H[SUP]1[/SUP], van Laarhoven HW, Pots JM, Duiveman-de Boer T, Mulder SF, van Herpen CM, Jacobs JF, Leer JW, Bleijenberg G, Stelma FF, Torensma R, de Vries IJ.
[h=3]Author information[/h]
[h=3]Abstract[/h] The aim of this study was to compare humoral and cellular immune responses to influenza vaccination in cancer survivors with and without severe symptoms of fatigue. Severely fatigued (n=15) and non-fatigued (n=12) disease-free cancer survivors were vaccinated against seasonal influenza. Humoral immunity was evaluated at baseline and post-vaccination by a hemagglutination inhibition assay. Cellular immunity was evaluated at baseline and post-vaccination by lymphocyte proliferation and activation assays. Regulatory T cells were measured at baseline by flow cytometry and heat-shock protein 90 levels by ELISA. Comparable humoral immune responses were observed in fatigued and non-fatigued patients, both pre- and post-vaccination. At baseline, fatigued patients showed a significantly diminished cellular proliferation upon virus stimulation with strain H3N2 (1414?1201 counts), and a trend in a similar direction with strain H1N1 (3025?2339 counts), compared to non-fatigued patients (3099?2401 and 5877?4604 counts, respectively). The percentage of regulatory T lymphocytes was significantly increased (4.4?2.1% versus 2.4?0.8%) and significantly lower amounts of interleukin 2 were detected prior to vaccination in fatigued compared to non-fatigued patients (36.3?44.3 pg/ml versus 94.0?45.4 pg/ml with strain H3N2 and 28.4?44.0 pg/ml versus 74.5?56.1 pg/ml with strain H1N1). Pre-vaccination heat-shock protein 90 concentrations, post-vaccination cellular proliferation, and post-vaccination cytokine concentrations did not differ between both groups. In conclusion, influenza vaccination is favorable for severely fatigued cancer survivors and should be recommended when indicated. However, compared to non-fatigued cancer survivors, fatigued cancer survivors showed several significant differences in immunological reactivity at baseline, which warrants further investigation.
[h=4]KEYWORDS:[/h] CBT cognitive behavior therapy; CIS-fatigue Checklist Individual Strength fatigue subscale; Cancer; Fatigue; HI hemagglutination-inhibition; HSP90α human heat shock protein 90 alpha; IFN- γ interferon gamma; IL-10 interleukin 10; IL-2 interleukin 2; IL-4 interleukin 4; IL-5 interleukin 5; Immunity; Influenza; PBMC peripheral blood mononuclear cells; PHA phytohemagglutinin; RUNMC Radboud University Nijmegen Medical Centre; Treg regulatory T lymphocytes; Vaccination
PMID: 25996472 [PubMed - as supplied by publisher]
[h=1]Humoral and cellular immune responses after influenza vaccination in patients with postcancer fatigue.[/h] Prinsen H[SUP]1[/SUP], van Laarhoven HW, Pots JM, Duiveman-de Boer T, Mulder SF, van Herpen CM, Jacobs JF, Leer JW, Bleijenberg G, Stelma FF, Torensma R, de Vries IJ.
[h=3]Author information[/h]
[h=3]Abstract[/h] The aim of this study was to compare humoral and cellular immune responses to influenza vaccination in cancer survivors with and without severe symptoms of fatigue. Severely fatigued (n=15) and non-fatigued (n=12) disease-free cancer survivors were vaccinated against seasonal influenza. Humoral immunity was evaluated at baseline and post-vaccination by a hemagglutination inhibition assay. Cellular immunity was evaluated at baseline and post-vaccination by lymphocyte proliferation and activation assays. Regulatory T cells were measured at baseline by flow cytometry and heat-shock protein 90 levels by ELISA. Comparable humoral immune responses were observed in fatigued and non-fatigued patients, both pre- and post-vaccination. At baseline, fatigued patients showed a significantly diminished cellular proliferation upon virus stimulation with strain H3N2 (1414?1201 counts), and a trend in a similar direction with strain H1N1 (3025?2339 counts), compared to non-fatigued patients (3099?2401 and 5877?4604 counts, respectively). The percentage of regulatory T lymphocytes was significantly increased (4.4?2.1% versus 2.4?0.8%) and significantly lower amounts of interleukin 2 were detected prior to vaccination in fatigued compared to non-fatigued patients (36.3?44.3 pg/ml versus 94.0?45.4 pg/ml with strain H3N2 and 28.4?44.0 pg/ml versus 74.5?56.1 pg/ml with strain H1N1). Pre-vaccination heat-shock protein 90 concentrations, post-vaccination cellular proliferation, and post-vaccination cytokine concentrations did not differ between both groups. In conclusion, influenza vaccination is favorable for severely fatigued cancer survivors and should be recommended when indicated. However, compared to non-fatigued cancer survivors, fatigued cancer survivors showed several significant differences in immunological reactivity at baseline, which warrants further investigation.
[h=4]KEYWORDS:[/h] CBT cognitive behavior therapy; CIS-fatigue Checklist Individual Strength fatigue subscale; Cancer; Fatigue; HI hemagglutination-inhibition; HSP90α human heat shock protein 90 alpha; IFN- γ interferon gamma; IL-10 interleukin 10; IL-2 interleukin 2; IL-4 interleukin 4; IL-5 interleukin 5; Immunity; Influenza; PBMC peripheral blood mononuclear cells; PHA phytohemagglutinin; RUNMC Radboud University Nijmegen Medical Centre; Treg regulatory T lymphocytes; Vaccination
PMID: 25996472 [PubMed - as supplied by publisher]