tetano
Editor, Senior Moderator
J Infect Dis. 2016 Dec 23. pii: jiw629. doi: 10.1093/infdis/jiw629. [Epub ahead of print]
[h=1]Human seasonal influenza A viruses induce H7N9-cross-reactive antibody-dependent cellular cytotoxicity (ADCC) antibodies that are directed towards the nucleoprotein.[/h] Jegaskanda S[SUP]1,[/SUP][SUP]2[/SUP], Co MD[SUP]2[/SUP], Cruz J[SUP]3[/SUP], Subbarao K[SUP]1[/SUP], Ennis FA[SUP]2[/SUP], Terajima M[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antibodies that mediate antibody-dependent cellular cytotoxicity (ADCC) against avian influenza virus subtypes including H7N9 and H5N1 have been detected in human sera. There are limited data on the presence of such cross-reactive ADCC mediating antibodies (ADCC-Abs) in children and the viral target(s) of these Abs are poorly understood. Using high-throughput NK cell activation and NK cytotoxicity assays, we compared ADCC-Abs in sera collected from healthy infants (≤1 year), children (2-17 years) and adults (≥18 years) against H7N9 virus-infected cells and recombinant HA, NA and NP proteins. High titers of ADCC-Abs against H7N9 virus-infected cells were detected in sera from adults and children but not infants. Despite this, adult, children and infants had low or undetectable ADCC-Abs titers directed against H7N9 HA or NA proteins. Further analysis showed that ADCC-Abs titers were significantly higher towards H7N9 NP, as compared to H7N9 HA or NA proteins and correlated strongly with ADCC-Abs titers against H7N9 virus-infected cells. Indeed, ADCC-Abs to NPs of seasonal H1N1 and H3N2 viruses correlated strongly with ADCC-Abs to H7N9 NP, suggesting that seasonal influenza infections and vaccinations may induce these cross-reactive antibodies. Targeting ADCC-Abs to internal proteins may be a potential mechanism of universal vaccine design.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.
[h=4]KEYWORDS:[/h] ADCC; antibody; antibody-dependent cellular cytotoxicity; immunity; influenza; influenza virus; nucleoprotein; vaccine
PMID: 28011910 DOI: 10.1093/infdis/jiw629
[PubMed - as supplied by publisher]
[h=1]Human seasonal influenza A viruses induce H7N9-cross-reactive antibody-dependent cellular cytotoxicity (ADCC) antibodies that are directed towards the nucleoprotein.[/h] Jegaskanda S[SUP]1,[/SUP][SUP]2[/SUP], Co MD[SUP]2[/SUP], Cruz J[SUP]3[/SUP], Subbarao K[SUP]1[/SUP], Ennis FA[SUP]2[/SUP], Terajima M[SUP]4[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] Antibodies that mediate antibody-dependent cellular cytotoxicity (ADCC) against avian influenza virus subtypes including H7N9 and H5N1 have been detected in human sera. There are limited data on the presence of such cross-reactive ADCC mediating antibodies (ADCC-Abs) in children and the viral target(s) of these Abs are poorly understood. Using high-throughput NK cell activation and NK cytotoxicity assays, we compared ADCC-Abs in sera collected from healthy infants (≤1 year), children (2-17 years) and adults (≥18 years) against H7N9 virus-infected cells and recombinant HA, NA and NP proteins. High titers of ADCC-Abs against H7N9 virus-infected cells were detected in sera from adults and children but not infants. Despite this, adult, children and infants had low or undetectable ADCC-Abs titers directed against H7N9 HA or NA proteins. Further analysis showed that ADCC-Abs titers were significantly higher towards H7N9 NP, as compared to H7N9 HA or NA proteins and correlated strongly with ADCC-Abs titers against H7N9 virus-infected cells. Indeed, ADCC-Abs to NPs of seasonal H1N1 and H3N2 viruses correlated strongly with ADCC-Abs to H7N9 NP, suggesting that seasonal influenza infections and vaccinations may induce these cross-reactive antibodies. Targeting ADCC-Abs to internal proteins may be a potential mechanism of universal vaccine design.
? The Author 2016. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail journals.permissions@oup.com.
[h=4]KEYWORDS:[/h] ADCC; antibody; antibody-dependent cellular cytotoxicity; immunity; influenza; influenza virus; nucleoprotein; vaccine
PMID: 28011910 DOI: 10.1093/infdis/jiw629
[PubMed - as supplied by publisher]