tetano
Editor, Senior Moderator
PLoS Pathog. 2012 Jan;8(1):e1002488. Epub 2012 Jan 19.
Human-like PB2 627K Influenza Virus Polymerase Activity Is Regulated by Importin-α1 and -α7.
Hudjetz B, Gabriel G.
Source
Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
Abstract
Influenza A viruses may cross species barriers and transmit to humans with the potential to cause pandemics. Interplay of human- (PB2 627K) and avian-like (PB2 627E) influenza polymerase complexes with unknown host factors have been postulated to play a key role in interspecies transmission. Here, we have identified human importin-α isoforms (α1 and α7) as positive regulators of human- but not avian-like polymerase activity. Human-like polymerase activity correlated with efficient recruitment of α1 and α7 to viral ribonucleoprotein complexes (vRNPs) without affecting subcellular localization. We also observed that human-like influenza virus growth was impaired in α1 and α7 downregulated human lung cells. Mice lacking α7 were less susceptible to human- but not avian-like influenza virus infection. Thus, α1 and α7 are positive regulators of human-like polymerase activity and pathogenicity beyond their role in nuclear transport.
PMID:
22275867
[PubMed - in process]
PMCID: PMC3262014
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22275867
Human-like PB2 627K Influenza Virus Polymerase Activity Is Regulated by Importin-α1 and -α7.
Hudjetz B, Gabriel G.
Source
Heinrich-Pette-Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.
Abstract
Influenza A viruses may cross species barriers and transmit to humans with the potential to cause pandemics. Interplay of human- (PB2 627K) and avian-like (PB2 627E) influenza polymerase complexes with unknown host factors have been postulated to play a key role in interspecies transmission. Here, we have identified human importin-α isoforms (α1 and α7) as positive regulators of human- but not avian-like polymerase activity. Human-like polymerase activity correlated with efficient recruitment of α1 and α7 to viral ribonucleoprotein complexes (vRNPs) without affecting subcellular localization. We also observed that human-like influenza virus growth was impaired in α1 and α7 downregulated human lung cells. Mice lacking α7 were less susceptible to human- but not avian-like influenza virus infection. Thus, α1 and α7 are positive regulators of human-like polymerase activity and pathogenicity beyond their role in nuclear transport.
PMID:
22275867
[PubMed - in process]
PMCID: PMC3262014
Free full text
http://www.ncbi.nlm.nih.gov/pubmed/22275867