tetano
Editor, Senior Moderator
Clin Transl Immunology. 2019 Sep 23;8(9):e1079. doi: 10.1002/cti2.1079. eCollection 2019. [h=1]Human γδ T-cell receptor repertoire is shaped by influenza viruses, age and tissue compartmentalisation.[/h]
Sant S[SUP]1[/SUP], Jenkins MR[SUP]2,[/SUP][SUP]3,[/SUP][SUP]4[/SUP], Dash P[SUP]5[/SUP], Watson KA[SUP]2[/SUP], Wang Z[SUP]1[/SUP], Pizzolla A[SUP]1[/SUP], Koutsakos M[SUP]1[/SUP], Nguyen TH[SUP]1[/SUP], Lappas M[SUP]6[/SUP], Crowe J[SUP]7[/SUP], Loudovaris T[SUP]8[/SUP], Mannering SI[SUP]8[/SUP], Westall GP[SUP]9[/SUP], Kotsimbos TC[SUP]10,[/SUP][SUP]11[/SUP], Cheng AC[SUP]12,[/SUP][SUP]13[/SUP], Wakim L[SUP]1[/SUP], Doherty PC[SUP]1,[/SUP][SUP]2[/SUP], Thomas PG[SUP]5[/SUP], Loh L[SUP]1[/SUP], Kedzierska K[SUP]1[/SUP].
[h=3]Author information[/h] 1 Department of Microbiology and Immunology University of Melbourne at The Peter Doherty Institute for Infection and Immunity Melbourne VIC Australia. 2 Immunology Division Walter and Eliza Hall Institute Melbourne VIC Australia. 3 LaTrobe Institute for Molecular Science La Trobe University Melbourne VIC Australia. 4 Department of Medical Biology The University of Melbourne Melbourne VIC Australia. 5 Department of Immunology St Jude Children's Research Hospital Memphis TN USA. 6 Obstetrics, Nutrition and Endocrinology Group Department of Obstetrics & Gynaecology Mercy Hospital for Women University of Melbourne Melbourne VIC Australia. 7 Deepdene Surgery Deepdene VIC Australia. 8 Immunology and Diabetes Unit St Vincent's Institute of Medical Research Fitzroy VIC Australia. 9 Lung Transplant Unit Alfred Hospital Melbourne VIC Australia. 10 Department of Allergy, Immunology and Respiratory Medicine The Alfred Hospital Melbourne VIC Australia. 11 Department of Medicine Central Clinical School The Alfred Hospital Melbourne Monash University Melbourne VIC Australia. 12 School of Public Health and Preventive Medicine Monash University Melbourne VIC Australia. 13 Infection Prevention and Healthcare Epidemiology Unit Alfred Health Melbourne VIC Australia.
[h=3]Abstract[/h] [h=4]Background:[/h] Although γδ T cells comprise up to 10% of human peripheral blood T cells, questions remain regarding their role in disease states and T-cell receptor (TCR) clonal expansions. We dissected anti-viral functions of human γδ T cells towards influenza viruses and defined influenza-reactive γδ TCRs in the context of γδ-TCRs across the human lifespan.
[h=4]Methods:[/h] We performed [SUP]51[/SUP]Cr-killing assay and single-cell time-lapse live video microscopy to define mechanisms underlying γδ T-cell-mediated killing of influenza-infected targets. We assessed cytotoxic profiles of γδ T cells in influenza-infected patients and IFN-γ production towards influenza-infected lung epithelial cells. Using single-cell RT-PCR, we characterised paired TCRγδ clonotypes for influenza-reactive γδ T cells in comparison with TCRs from healthy neonates, adults, elderly donors and tissues.
[h=4]Results:[/h] We provide the first visual evidence of γδ T-cell-mediated killing of influenza-infected targets and show distinct features to those reported for CD8[SUP]+[/SUP] T cells. γδ T cells displayed poly-cytotoxic profiles in influenza-infected patients and produced IFN-γ towards influenza-infected cells. These IFN-γ-producing γδ T cells were skewed towards the γ9δ2 TCRs, particularly expressing the public GV9-TCRγ, capable of pairing with numerous TCR-δ chains, suggesting their significant role in γδ T-cell immunity. Neonatal γδ T cells displayed extensive non-overlapping TCRγδ repertoires, while adults had enriched γ9δ2-pairings with diverse CDR3γδ regions. Conversely, the elderly showed distinct γδ-pairings characterised by large clonal expansions, a profile also prominent in adult tissues.
[h=4]Conclusion:[/h] Human TCRγδ repertoire is shaped by age, tissue compartmentalisation and the individual's history of infection, suggesting that these somewhat enigmatic γδ T cells indeed respond to antigen challenge.
[h=4]KEYWORDS:[/h] human tissues; human γδ T cells; influenza virus infection; paired TCRγδ repertoire; public GV9‐TCRγ clonotype
PMID: 31559018 PMCID: PMC6756999 DOI: 10.1002/cti2.1079
Free PMC Article
Sant S[SUP]1[/SUP], Jenkins MR[SUP]2,[/SUP][SUP]3,[/SUP][SUP]4[/SUP], Dash P[SUP]5[/SUP], Watson KA[SUP]2[/SUP], Wang Z[SUP]1[/SUP], Pizzolla A[SUP]1[/SUP], Koutsakos M[SUP]1[/SUP], Nguyen TH[SUP]1[/SUP], Lappas M[SUP]6[/SUP], Crowe J[SUP]7[/SUP], Loudovaris T[SUP]8[/SUP], Mannering SI[SUP]8[/SUP], Westall GP[SUP]9[/SUP], Kotsimbos TC[SUP]10,[/SUP][SUP]11[/SUP], Cheng AC[SUP]12,[/SUP][SUP]13[/SUP], Wakim L[SUP]1[/SUP], Doherty PC[SUP]1,[/SUP][SUP]2[/SUP], Thomas PG[SUP]5[/SUP], Loh L[SUP]1[/SUP], Kedzierska K[SUP]1[/SUP].
[h=3]Author information[/h] 1 Department of Microbiology and Immunology University of Melbourne at The Peter Doherty Institute for Infection and Immunity Melbourne VIC Australia. 2 Immunology Division Walter and Eliza Hall Institute Melbourne VIC Australia. 3 LaTrobe Institute for Molecular Science La Trobe University Melbourne VIC Australia. 4 Department of Medical Biology The University of Melbourne Melbourne VIC Australia. 5 Department of Immunology St Jude Children's Research Hospital Memphis TN USA. 6 Obstetrics, Nutrition and Endocrinology Group Department of Obstetrics & Gynaecology Mercy Hospital for Women University of Melbourne Melbourne VIC Australia. 7 Deepdene Surgery Deepdene VIC Australia. 8 Immunology and Diabetes Unit St Vincent's Institute of Medical Research Fitzroy VIC Australia. 9 Lung Transplant Unit Alfred Hospital Melbourne VIC Australia. 10 Department of Allergy, Immunology and Respiratory Medicine The Alfred Hospital Melbourne VIC Australia. 11 Department of Medicine Central Clinical School The Alfred Hospital Melbourne Monash University Melbourne VIC Australia. 12 School of Public Health and Preventive Medicine Monash University Melbourne VIC Australia. 13 Infection Prevention and Healthcare Epidemiology Unit Alfred Health Melbourne VIC Australia.
[h=3]Abstract[/h] [h=4]Background:[/h] Although γδ T cells comprise up to 10% of human peripheral blood T cells, questions remain regarding their role in disease states and T-cell receptor (TCR) clonal expansions. We dissected anti-viral functions of human γδ T cells towards influenza viruses and defined influenza-reactive γδ TCRs in the context of γδ-TCRs across the human lifespan.
[h=4]Methods:[/h] We performed [SUP]51[/SUP]Cr-killing assay and single-cell time-lapse live video microscopy to define mechanisms underlying γδ T-cell-mediated killing of influenza-infected targets. We assessed cytotoxic profiles of γδ T cells in influenza-infected patients and IFN-γ production towards influenza-infected lung epithelial cells. Using single-cell RT-PCR, we characterised paired TCRγδ clonotypes for influenza-reactive γδ T cells in comparison with TCRs from healthy neonates, adults, elderly donors and tissues.
[h=4]Results:[/h] We provide the first visual evidence of γδ T-cell-mediated killing of influenza-infected targets and show distinct features to those reported for CD8[SUP]+[/SUP] T cells. γδ T cells displayed poly-cytotoxic profiles in influenza-infected patients and produced IFN-γ towards influenza-infected cells. These IFN-γ-producing γδ T cells were skewed towards the γ9δ2 TCRs, particularly expressing the public GV9-TCRγ, capable of pairing with numerous TCR-δ chains, suggesting their significant role in γδ T-cell immunity. Neonatal γδ T cells displayed extensive non-overlapping TCRγδ repertoires, while adults had enriched γ9δ2-pairings with diverse CDR3γδ regions. Conversely, the elderly showed distinct γδ-pairings characterised by large clonal expansions, a profile also prominent in adult tissues.
[h=4]Conclusion:[/h] Human TCRγδ repertoire is shaped by age, tissue compartmentalisation and the individual's history of infection, suggesting that these somewhat enigmatic γδ T cells indeed respond to antigen challenge.
[h=4]KEYWORDS:[/h] human tissues; human γδ T cells; influenza virus infection; paired TCRγδ repertoire; public GV9‐TCRγ clonotype
PMID: 31559018 PMCID: PMC6756999 DOI: 10.1002/cti2.1079
Free PMC Article