tetano
Editor, Senior Moderator
AIDS Res Hum Retroviruses. 2012 Jun 26. [Epub ahead of print]
HIV-1 Accumulates in Influenza-specific T-cells in Subjects Receiving Seasonal Vaccination in the Context of Effective Antiretroviral Therapy.
Jones B, Kovacs C, Chun TW, Ostrowski M.
Source
University of Toronto, Immunology, Rm 6352, 1 King's College Cir, Toronto, Ontario, Canada, M5S 1A8, 416-946-0277 ; bjones.ut@gmail.com.
Abstract
Whether or not HIV-1 continues to infect cells in individuals treated with effective antiretroviral therapy (ART) remains controversial. Here, we determined whether the redistribution of the HIV-1 proviral burden with respect to antigen specificity of CD4+ cells would provide evidence for ongoing infection cycles in vivo. HIV-1 preferentially infects antigen-stimulated CD4+ T-cells. In the setting of prolonged effective ART, we postulated that, if infection cycles were occurring, influenza-specific CD4+ T cells, activated by influenza vaccination, would preferentially accumulate proviral burden. Peripheral blood mononuclear cells (PBMC) were collected from HIV-1-infected subjects, who had been treated with effective ART for >5 years, before and after influenza vaccination. CD4+ T-cells were sorted by antigen-specificity and HIV-1 proviral burdens determined. Levels of HIV-1 production upon in vitro antigenic stimulation were also measured. At baseline, influenza-specific CD4+ T-cells carried higher HIV-1 proviral loads than HIV-1-p55-specific CD4+ T-cells. Upon influenza vaccination we observed trends towards elevated levels of HIV-1 proviral DNA in influenza and HIV-1-p55-specific, but not tetanus toxoid or CMV-specific CD4+ T-cells. Higher levels of HIV-1 virions were produced upon influenza stimulation in post-vaccination as compared to baseline samples. While the trends towards increased proviral burdens in influenza-specific cells failed to reach statistical significance, our observation of disproportionately high-levels of provirus in influenza-specific cells at baseline supports that this represents a real increase which is cumulative over multiple annual vaccinations. This has implications for the eradication of HIV-1 by adding to the evidence that the resting CD4+ T-cell viral reservoir is continually replenished in ART-treated subjects.
PMID:
22734882
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22734882
HIV-1 Accumulates in Influenza-specific T-cells in Subjects Receiving Seasonal Vaccination in the Context of Effective Antiretroviral Therapy.
Jones B, Kovacs C, Chun TW, Ostrowski M.
Source
University of Toronto, Immunology, Rm 6352, 1 King's College Cir, Toronto, Ontario, Canada, M5S 1A8, 416-946-0277 ; bjones.ut@gmail.com.
Abstract
Whether or not HIV-1 continues to infect cells in individuals treated with effective antiretroviral therapy (ART) remains controversial. Here, we determined whether the redistribution of the HIV-1 proviral burden with respect to antigen specificity of CD4+ cells would provide evidence for ongoing infection cycles in vivo. HIV-1 preferentially infects antigen-stimulated CD4+ T-cells. In the setting of prolonged effective ART, we postulated that, if infection cycles were occurring, influenza-specific CD4+ T cells, activated by influenza vaccination, would preferentially accumulate proviral burden. Peripheral blood mononuclear cells (PBMC) were collected from HIV-1-infected subjects, who had been treated with effective ART for >5 years, before and after influenza vaccination. CD4+ T-cells were sorted by antigen-specificity and HIV-1 proviral burdens determined. Levels of HIV-1 production upon in vitro antigenic stimulation were also measured. At baseline, influenza-specific CD4+ T-cells carried higher HIV-1 proviral loads than HIV-1-p55-specific CD4+ T-cells. Upon influenza vaccination we observed trends towards elevated levels of HIV-1 proviral DNA in influenza and HIV-1-p55-specific, but not tetanus toxoid or CMV-specific CD4+ T-cells. Higher levels of HIV-1 virions were produced upon influenza stimulation in post-vaccination as compared to baseline samples. While the trends towards increased proviral burdens in influenza-specific cells failed to reach statistical significance, our observation of disproportionately high-levels of provirus in influenza-specific cells at baseline supports that this represents a real increase which is cumulative over multiple annual vaccinations. This has implications for the eradication of HIV-1 by adding to the evidence that the resting CD4+ T-cell viral reservoir is continually replenished in ART-treated subjects.
PMID:
22734882
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22734882