tetano
Editor, Senior Moderator
ACS Omega. 2019 Aug 26;4(11):14560-14568. doi: 10.1021/acsomega.9b01790. eCollection 2019 Sep 10.
[h=1]Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker.[/h] Lim J[SUP]1,[/SUP][SUP]2[/SUP], Byun J[SUP]1[/SUP], Guk K[SUP]1,[/SUP][SUP]2[/SUP], Hwang SG[SUP]1,[/SUP][SUP]2[/SUP], Bae PK[SUP]3[/SUP], Jung J[SUP]1,[/SUP][SUP]2[/SUP], Kang T[SUP]1,[/SUP][SUP]2[/SUP], Lim EK[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h] 1 BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. 2 Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. 3 BioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
[h=3]Abstract[/h] Several microRNAs (miRNAs) have been reported to be closely related to influenza A virus infection, replication, and immune response. Therefore, the development of the infectious-disease detection system using miRNAs as biomarkers is actively underway. Herein, we identified two miRNAs (miR-181c-5p and miR-1254) as biomarkers for detection of pandemic influenza A H1N1 virus infection and proposed the catalytic hairpin assembly-based in vitro diagnostic (CIVD) system for a highly sensitive diagnosis; this system is composed of two sets of cascade hairpin probes enabling to detect miR-181c-5p and miR-1254. We demonstrated that CIVD kits could not only detect subnanomolar levels of target miRNAs but also distinguish even single-base mismatches. Moreover, this CIVD kit has shown excellent detection performance in real intracellular RNA samples and confirmed results similar to those of conventional methods (microarray and quantitative real-time polymerase chain reaction).
PMID: 31528810 PMCID: PMC6740188 DOI: 10.1021/acsomega.9b01790
Free PMC Article
[h=1]Highly Sensitive in Vitro Diagnostic System of Pandemic Influenza A (H1N1) Virus Infection with Specific MicroRNA as a Biomarker.[/h] Lim J[SUP]1,[/SUP][SUP]2[/SUP], Byun J[SUP]1[/SUP], Guk K[SUP]1,[/SUP][SUP]2[/SUP], Hwang SG[SUP]1,[/SUP][SUP]2[/SUP], Bae PK[SUP]3[/SUP], Jung J[SUP]1,[/SUP][SUP]2[/SUP], Kang T[SUP]1,[/SUP][SUP]2[/SUP], Lim EK[SUP]1,[/SUP][SUP]2[/SUP].
[h=3]Author information[/h] 1 BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. 2 Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. 3 BioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
[h=3]Abstract[/h] Several microRNAs (miRNAs) have been reported to be closely related to influenza A virus infection, replication, and immune response. Therefore, the development of the infectious-disease detection system using miRNAs as biomarkers is actively underway. Herein, we identified two miRNAs (miR-181c-5p and miR-1254) as biomarkers for detection of pandemic influenza A H1N1 virus infection and proposed the catalytic hairpin assembly-based in vitro diagnostic (CIVD) system for a highly sensitive diagnosis; this system is composed of two sets of cascade hairpin probes enabling to detect miR-181c-5p and miR-1254. We demonstrated that CIVD kits could not only detect subnanomolar levels of target miRNAs but also distinguish even single-base mismatches. Moreover, this CIVD kit has shown excellent detection performance in real intracellular RNA samples and confirmed results similar to those of conventional methods (microarray and quantitative real-time polymerase chain reaction).
PMID: 31528810 PMCID: PMC6740188 DOI: 10.1021/acsomega.9b01790
Free PMC Article