tetano
Editor, Senior Moderator
J Infect Dis. 2012 Oct 29. [Epub ahead of print]
Heterovariant cross-reactive B-cell responses induced by the 2009 pandemic influenza A/H1N1 vaccine.
He XS, Sasaki S, Baer J, Khurana S, Golding H, Treanor JJ, Topham DJ, Sangster MY, Jin H, Dekker CL, Subbarao K, Greenberg HB.
Source
Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305.
Abstract
Background. The generation of heterovariant immunity is a highly desirable feature of influenza vaccines. The goal of this study was to compare the heterovariant B cell response induced by the monovalent inactivated 2009 pandemic influenza A/H1N1 (pH1N1) vaccine with that induced by the 2009 seasonal trivalent influenza vaccine (sTIV) containing a seasonal A/H1N1 (sH1N1) component in young and elderly adults.Methods. Plasmablast-derived polyclonal antibodies (PPAb) from young and elderly recipients of pH1N1 vaccine or sTIV were tested for binding activity to various influenza antigens.Results. In pH1N1 recipients, the PPAb titers against homotypic pH1N1 were similar to those against the heterovariant sH1N1 and were similar between young and elderly subjects. The PPAb avidity was higher in elderly than young individuals. In contrast the young sTIV recipients had 10-fold lower heterovariant PPAb titers against pH1N1 than against the homotypic sH1N1 vaccine. In binding assays with recombinant head and stalk domains of hemagglutinin, PPAb of the pH1N1 recipients, but not PPAb of the sTIV recipients, bound to the conserved stalk domain.Conclusion. The pH1N1 vaccine induced production of PPAb with heterovariant reactivity, including antibodies targeting the conserved hemagglutinin stalk domain.
PMID:
23107783
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23107783
Heterovariant cross-reactive B-cell responses induced by the 2009 pandemic influenza A/H1N1 vaccine.
He XS, Sasaki S, Baer J, Khurana S, Golding H, Treanor JJ, Topham DJ, Sangster MY, Jin H, Dekker CL, Subbarao K, Greenberg HB.
Source
Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305.
Abstract
Background. The generation of heterovariant immunity is a highly desirable feature of influenza vaccines. The goal of this study was to compare the heterovariant B cell response induced by the monovalent inactivated 2009 pandemic influenza A/H1N1 (pH1N1) vaccine with that induced by the 2009 seasonal trivalent influenza vaccine (sTIV) containing a seasonal A/H1N1 (sH1N1) component in young and elderly adults.Methods. Plasmablast-derived polyclonal antibodies (PPAb) from young and elderly recipients of pH1N1 vaccine or sTIV were tested for binding activity to various influenza antigens.Results. In pH1N1 recipients, the PPAb titers against homotypic pH1N1 were similar to those against the heterovariant sH1N1 and were similar between young and elderly subjects. The PPAb avidity was higher in elderly than young individuals. In contrast the young sTIV recipients had 10-fold lower heterovariant PPAb titers against pH1N1 than against the homotypic sH1N1 vaccine. In binding assays with recombinant head and stalk domains of hemagglutinin, PPAb of the pH1N1 recipients, but not PPAb of the sTIV recipients, bound to the conserved stalk domain.Conclusion. The pH1N1 vaccine induced production of PPAb with heterovariant reactivity, including antibodies targeting the conserved hemagglutinin stalk domain.
PMID:
23107783
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23107783