tetano
Editor, Senior Moderator
Biotechnol J. 2013 Jul 1. doi: 10.1002/biot.201300116. [Epub ahead of print]
Heterologous prime-boost immunization regimens using adenovirus vector and virus-like particles induce broadly neutralizing antibodies against H5N1 avian influenza viruses.
Lin SC, Liu WC, Lin YF, Huang YH, Liu JH, Wu SC.
Source
Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan.
Abstract
Highly pathogenic avian influenza (HPAI) H5N1 viruses continue to trigger severe diseases in poultry and humans, prompting many efforts to develop an effective vaccine. Toward that goal, we employed two modern sophisticated techniques namely construction of recombinant adenovirus vector encoding HA (rAd-HA) and flagellin-containing virus-like particle (FliC-VLP). Using a murine model, we investigated a heterologous prime-boost regimen of rAd-HA vector and FliC-VLP in contrast to two-dose immunizations using rAd-HA or FliC-VLP alone. Our results indicate that priming with rAd-HA vector followed by a FliC-VLP booster induced the highest levels of HA-specific total IgG, IgG1, IgG2a, hemagglutination inhibition and neutralizing antibody titers against homologous and heterologous clades of H5N1 virus strains. These results provide useful information to support the development of more effective H5N1 vaccines.
Copyright ? 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KEYWORDS:
H5N1 vaccine, VLP, adenovirus, prime-boost immunization
PMID:
23813782
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23813782
Heterologous prime-boost immunization regimens using adenovirus vector and virus-like particles induce broadly neutralizing antibodies against H5N1 avian influenza viruses.
Lin SC, Liu WC, Lin YF, Huang YH, Liu JH, Wu SC.
Source
Institute of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan.
Abstract
Highly pathogenic avian influenza (HPAI) H5N1 viruses continue to trigger severe diseases in poultry and humans, prompting many efforts to develop an effective vaccine. Toward that goal, we employed two modern sophisticated techniques namely construction of recombinant adenovirus vector encoding HA (rAd-HA) and flagellin-containing virus-like particle (FliC-VLP). Using a murine model, we investigated a heterologous prime-boost regimen of rAd-HA vector and FliC-VLP in contrast to two-dose immunizations using rAd-HA or FliC-VLP alone. Our results indicate that priming with rAd-HA vector followed by a FliC-VLP booster induced the highest levels of HA-specific total IgG, IgG1, IgG2a, hemagglutination inhibition and neutralizing antibody titers against homologous and heterologous clades of H5N1 virus strains. These results provide useful information to support the development of more effective H5N1 vaccines.
Copyright ? 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
KEYWORDS:
H5N1 vaccine, VLP, adenovirus, prime-boost immunization
PMID:
23813782
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/23813782