tetano
Editor, Senior Moderator
Heliyon
. 2022 Oct 3;8(10):e10858.
doi: 10.1016/j.heliyon.2022.e10858. eCollection 2022 Oct.
Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives
Kang-Sheng Liu[SUP] 1 [/SUP], Xiao-Dong Mao[SUP] 2 3 [/SUP], Wenjing Ni[SUP] 2 3 [/SUP], Tai-Ping Li[SUP] 4 [/SUP]
Affiliations
Abstract
Nowadays, coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), whose infectivity is awfully strong, has been a major global threat to the public health. Since lung is the major target of SARS-CoV-2, the infection can lead to respiratory distress syndrome (RDS), multiple organ failure (MOF), and even death. The studies on viral structure and infection mechanism have found that angiotensin-converting enzyme 2 (ACE2), a pivotal enzyme affecting the organ-targeting in the RAS system, is the receptor of the SARS-CoV-2 virus. Currently, the detection of SARSCoV-2 is mainly achieved using open plate real-time reverse-transcription polymerase chain reaction (RT-PCR). While open plate method has some limitations, such as a high false-negative rate, cumbersome manual operation, aerosol pollution and leakage risks. Therefore, a convenient method to rapidly detect SARS-CoV-2 virus is urgently and extremely required for timely epidemic control with the limited resources. In this review, the current real-time methods and principles for novel coronavirus detection are summarized, with the aim to provide a reference for real-time screening of coronavirus in areas with insufficient detection capacity and inadequate medical resources. The development and establishment of a rapid, simple, sensitive and specific system to detect SARS-CoV-2 is of vital importance for distinct diagnosis and effective treatment of the virus, especially in the flu season.
Keywords: Gene editing techniques; Gene sequencing; Novel coronavirus; Nucleic acid testing; SARS-CoV-2.
. 2022 Oct 3;8(10):e10858.
doi: 10.1016/j.heliyon.2022.e10858. eCollection 2022 Oct.
Laboratory detection of SARS-CoV-2: A review of the current literature and future perspectives
Kang-Sheng Liu[SUP] 1 [/SUP], Xiao-Dong Mao[SUP] 2 3 [/SUP], Wenjing Ni[SUP] 2 3 [/SUP], Tai-Ping Li[SUP] 4 [/SUP]
Affiliations
- PMID: 36212015
- PMCID: PMC9527186
- DOI: 10.1016/j.heliyon.2022.e10858
Abstract
Nowadays, coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), whose infectivity is awfully strong, has been a major global threat to the public health. Since lung is the major target of SARS-CoV-2, the infection can lead to respiratory distress syndrome (RDS), multiple organ failure (MOF), and even death. The studies on viral structure and infection mechanism have found that angiotensin-converting enzyme 2 (ACE2), a pivotal enzyme affecting the organ-targeting in the RAS system, is the receptor of the SARS-CoV-2 virus. Currently, the detection of SARSCoV-2 is mainly achieved using open plate real-time reverse-transcription polymerase chain reaction (RT-PCR). While open plate method has some limitations, such as a high false-negative rate, cumbersome manual operation, aerosol pollution and leakage risks. Therefore, a convenient method to rapidly detect SARS-CoV-2 virus is urgently and extremely required for timely epidemic control with the limited resources. In this review, the current real-time methods and principles for novel coronavirus detection are summarized, with the aim to provide a reference for real-time screening of coronavirus in areas with insufficient detection capacity and inadequate medical resources. The development and establishment of a rapid, simple, sensitive and specific system to detect SARS-CoV-2 is of vital importance for distinct diagnosis and effective treatment of the virus, especially in the flu season.
Keywords: Gene editing techniques; Gene sequencing; Novel coronavirus; Nucleic acid testing; SARS-CoV-2.