tetano
Editor, Senior Moderator
Vaccine. 2014 Mar 31. pii: S0264-410X(14)00414-9. doi: 10.1016/j.vaccine.2014.03.045. [Epub ahead of print]
Heat shock protein gp96 adjuvant induces T cell responses and cross-protection to a split influenza vaccine.
Ju Y1, Fan H1, Liu J1, Hu J1, Li X1, Li C1, Chen L1, Gao Q2, Gao GF1, Meng S3.
Author information
Abstract
The commonly used inactivated or split influenza vaccines induce only induce minimal T cell responses and are less effective in preventing heterologous virus infection. Thus, developing cross-protective influenza vaccines against the spread of a new influenza virus is an important strategy against pandemic emergence. Here we demonstrated that immunization with heat shock protein gp96 as adjuvant led to a dramatic increased antigen-specific T cell response to a pandemic H1N1 split vaccine. Notably, gp96 elicited a cross-protective CD8+ T cell response to the internal conserved viral protein NP. Although the split pH1N1vaccine alone has low cross-protective efficiency, adding gp96 as an adjuvant effectively improved the cross-protection against challenge with a heterologous virus in mice. Our study reveals the novel property of gp96 in boosting the T cell response against conserved epitopes of influenza virus and its potential use as an adjuvant for human pre-pandemic inactivated influenza vaccines against different viral subtypes.
Copyright ? 2014 Elsevier Ltd. All rights reserved.
KEYWORDS:
Conserved epitope, Cross protection, Influenza vaccine, T cell response, gp96
PMID:
24699472
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24699472
Heat shock protein gp96 adjuvant induces T cell responses and cross-protection to a split influenza vaccine.
Ju Y1, Fan H1, Liu J1, Hu J1, Li X1, Li C1, Chen L1, Gao Q2, Gao GF1, Meng S3.
Author information
Abstract
The commonly used inactivated or split influenza vaccines induce only induce minimal T cell responses and are less effective in preventing heterologous virus infection. Thus, developing cross-protective influenza vaccines against the spread of a new influenza virus is an important strategy against pandemic emergence. Here we demonstrated that immunization with heat shock protein gp96 as adjuvant led to a dramatic increased antigen-specific T cell response to a pandemic H1N1 split vaccine. Notably, gp96 elicited a cross-protective CD8+ T cell response to the internal conserved viral protein NP. Although the split pH1N1vaccine alone has low cross-protective efficiency, adding gp96 as an adjuvant effectively improved the cross-protection against challenge with a heterologous virus in mice. Our study reveals the novel property of gp96 in boosting the T cell response against conserved epitopes of influenza virus and its potential use as an adjuvant for human pre-pandemic inactivated influenza vaccines against different viral subtypes.
Copyright ? 2014 Elsevier Ltd. All rights reserved.
KEYWORDS:
Conserved epitope, Cross protection, Influenza vaccine, T cell response, gp96
PMID:
24699472
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24699472