H5N1 Virus Pictures

[gsgs]

see this picture:

http://web.uct.ac.za/depts/mmi/stannard/fluvirus.html

the description is:

> The viral genome is composed of eight segments of ssRNA.
> The helical ribonucleo-protein is not often seen,
> but occasional particles show evidence of internal helical components.

I'm not sure : what/where is the helical ribonuclein ?
Do we see 2 viruses ? I do see the hemagglutinin spikes.

They are filamentous and the bigger right one is bended.
The horizontal "lines" inside that virus are the 8 ribonucleins ?
But this should be 3-dimensional, so maybe they are torus - like ?

They say "helical", so maybe one ribonucleoprotein is very long ? (how long ?)
how many ribonucleoproteins can we see on the picture ?

-----------edit 1--------------
seems there are 3 viruses on that picture

 

[Sally Furniss]

Re: pictures

Re: pictures

Bird Flu Virus Picture (H5N1)<!-- InstanceEndEditable -->

Return to <!-- InstanceBeginEditable name="EditRegion4" -->Bird Flu Virus Pictures from CDC<!-- InstanceEndEditable --> | <!-- InstanceBeginEditable name="EditRegion5" -->Hardin MD : Bird Flu (Many more pictures)<!-- InstanceEndEditable -->
<table border="0" cellpadding="5" width="750"> <tbody><tr> <td width=""><!-- InstanceBeginEditable name="EditRegion8" --> <table border="1" bordercolor="#000000" cellpadding="0" cellspacing="0" width="500"> <tbody><tr> <td width="100">

​

</td> <td width="100">

​

</td> <td width="100">

​

</td> <td width="100">

​

</td> <td width="100">

​

</td> </tr> <tr> <td>

H5N1​

</td> <td>

H5N1​

</td> <td>

H5N1 *​

</td> <td>

1918 Flu​

</td> <td>

1918 Flu​

</td> </tr> </tbody></table>

<!-- InstanceEndEditable --></td> <td valign="top" width="3">
</td> <td valign="top" width="209">
</td> </tr> <tr> <td valign="top"> <!-- InstanceBeginEditable name="EditRegion12" --> This transmission electron micrograph (TEM), taken at a magnification of 108,000x, revealed the ultrastructural details of two avian influenza A (H5N1) virions, a type of bird flu virus, which is a subtype of avian influenza A. At this magnification, one may note the stippled appearance of the roughened surface of the proteinaceous coat encasing each virion.
Although this virus does not typically infect humans, in 1997, the first instance of direct bird-to-human spread of influenza A (H5N1) virus was documented during an outbreak of avian influenza among poultry in Hong Kong. The virus caused severe respiratory illness in 18 people, of whom, 6 had died. Since that time, there have been other instances of H5N1 infection among humans.
During August to October, 2004, sporadic human cases of avian influenza A (H5N1) were reported in Vietnam and Thailand. Since December, 2004, a resurgence of poultry outbreaks and human cases has been reported in Vietnam. On February 2, 2005, the first of four human cases of H5N1 infection from Cambodia was reported. On July 21, 2005, the first human case of H5N1 in Indonesia was reported, and since then Indonesia has continued to report human cases in August, September, and October, 2005.
Content Providers(s): CDC/ Cynthia Goldsmith/ Jackie Katz
Picture & text from CDC/PHIL. For more information see Bird Flu Virus Pictures from CDC
</td></tr></tbody></table>

http://www.lib.uiowa.edu/hardin/md/cdc/8036.html

 

[gsgs]

Re: pictures

Re: pictures

in that long filamentous virus, there would be space
for ample nucleoproteins !
(do you think, you can spot any ?)
I wonder whether there still are only
8 (-11) ?


I have a gallery here:
http://health.ph.groups.yahoo.com/group/Flu_Pandemic/photos/browse/cf89
maybe you have to subscribe to yahoogroups to see it ?
maybe we should copy it to FT ?


testing :

does it work ?


http://us.a2.yahoofs.com/groups/g_15719278/cf89/__sr_/1212.jpg?grgQNBIB_2Y5rNDp

http://web.uct.ac.za/depts/mmi/stannard/flu3c.gif
http://web.uct.ac.za/depts/mmi/stannard/flucolo3.gif
http://web.uct.ac.za/depts/mmi/stannard/fluhelx2.gif
http://web.uct.ac.za/depts/mmi/stannard/flu3s.gif
http://www.virology.net/Big_Virology/EM/virus.gif
http://www.virology.net/Big_Virology/EM/influsphere.gif
http://www.virology.net/Big_Virology/BVRNAortho.html

 

[Sally Furniss]

Re: pictures

Re: pictures

Sure copy it here. Then we can all see.

 

[Anne]

Re: pictures

Re: pictures

links work, thank you for this pictures
RNP is RNA plus NP


Nucleoprotein
Also known as:
Nucleocapsid protein
Protein N

http://beta.uniprot.org/uniprot/O92784

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins.

It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export.
M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus