tetano
Editor, Senior Moderator
J Virol. 2015 Nov 25. pii: JVI.02332-15. [Epub ahead of print]
[h=1]H1N1 Swine Influenza Viruses Differ from Avian Precursors by a higher pH Optimum of Membrane Fusion.[/h] Baumann J[SUP]1[/SUP], Mounogou Kouassi N[SUP]1[/SUP], Foni E[SUP]2[/SUP], Klenk HD[SUP]1[/SUP], Matrosovich M[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The H1N1 Eurasian avian-like swine (EAsw) influenza viruses originated from an avian H1N1 virus. To characterize potential changes in the membrane fusion activity of the HA during avian-to-swine adaptation of the virus, we studied EAsw viruses isolated in the first years of their circulation in pigs and closely related contemporary H1N1 viruses of wild aquatic birds. Compared to the avian viruses, the swine viruses were less sensitive to neutralization by lysosomotropic agent NH[SUB]4[/SUB]Cl in MDCK cells, had a higher pH optimum of hemolytic activity and were less stable at acidic pH. Eight amino acid substitutions in the HA were found to separate the EAsw viruses from their putative avian precursor; four substitutions, T49[SUB]2[/SUB]S, N72[SUB]2[/SUB]D, R75[SUB]2[/SUB]K and S113[SUB]2[/SUB]F, were located in the structural regions of the HA2 subunit known to play a role in acid-induced conformational transition of the HA. We also studied low-pH-induced syncytia formation by cell-expressed HA proteins and found that the HAs of the 1918, 1957, 1968 and 2009 pandemic viruses required a lower pH for fusion induction as compared to the HA of a representative EAsw virus. Our data show that transmission of an avian H1N1 virus to pigs was accompanied by changes in conformational stability and fusion-promotion activity of the HA. We conclude that distinctive host-determined fusion characteristics of the HA may represent a barrier for avian-to-swine and swine-to-human transmission of influenza viruses.
[h=4]IMPORTANCE:[/h] Continuing cases of human infections with zoonotic influenza viruses highlight the necessity to understand which viral properties contribute to interspecies transmission. Efficient binding of the HA to cellular receptors in a new host species is known to be essential for the transmission. Less is known about required adaptive changes in the membrane fusion activity of the HA. Here we show that adaptation of an avian influenza virus to pigs in Europe in 1980s was accompanied by mutations in the HA which decreased its conformational stability and increased pH optimum of membrane fusion activity. This finding represents the first formal evidence of alteration of the HA fusion activity/stability during interspecies transmission of influenza viruses under natural settings.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26608319 [PubMed - as supplied by publisher]
[h=1]H1N1 Swine Influenza Viruses Differ from Avian Precursors by a higher pH Optimum of Membrane Fusion.[/h] Baumann J[SUP]1[/SUP], Mounogou Kouassi N[SUP]1[/SUP], Foni E[SUP]2[/SUP], Klenk HD[SUP]1[/SUP], Matrosovich M[SUP]3[/SUP].
[h=3]Author information[/h]
[h=3]Abstract[/h] The H1N1 Eurasian avian-like swine (EAsw) influenza viruses originated from an avian H1N1 virus. To characterize potential changes in the membrane fusion activity of the HA during avian-to-swine adaptation of the virus, we studied EAsw viruses isolated in the first years of their circulation in pigs and closely related contemporary H1N1 viruses of wild aquatic birds. Compared to the avian viruses, the swine viruses were less sensitive to neutralization by lysosomotropic agent NH[SUB]4[/SUB]Cl in MDCK cells, had a higher pH optimum of hemolytic activity and were less stable at acidic pH. Eight amino acid substitutions in the HA were found to separate the EAsw viruses from their putative avian precursor; four substitutions, T49[SUB]2[/SUB]S, N72[SUB]2[/SUB]D, R75[SUB]2[/SUB]K and S113[SUB]2[/SUB]F, were located in the structural regions of the HA2 subunit known to play a role in acid-induced conformational transition of the HA. We also studied low-pH-induced syncytia formation by cell-expressed HA proteins and found that the HAs of the 1918, 1957, 1968 and 2009 pandemic viruses required a lower pH for fusion induction as compared to the HA of a representative EAsw virus. Our data show that transmission of an avian H1N1 virus to pigs was accompanied by changes in conformational stability and fusion-promotion activity of the HA. We conclude that distinctive host-determined fusion characteristics of the HA may represent a barrier for avian-to-swine and swine-to-human transmission of influenza viruses.
[h=4]IMPORTANCE:[/h] Continuing cases of human infections with zoonotic influenza viruses highlight the necessity to understand which viral properties contribute to interspecies transmission. Efficient binding of the HA to cellular receptors in a new host species is known to be essential for the transmission. Less is known about required adaptive changes in the membrane fusion activity of the HA. Here we show that adaptation of an avian influenza virus to pigs in Europe in 1980s was accompanied by mutations in the HA which decreased its conformational stability and increased pH optimum of membrane fusion activity. This finding represents the first formal evidence of alteration of the HA fusion activity/stability during interspecies transmission of influenza viruses under natural settings.
Copyright ? 2015, American Society for Microbiology. All Rights Reserved.
PMID: 26608319 [PubMed - as supplied by publisher]