tetano
Editor, Senior Moderator
Am J Reprod Immunol. 2012 Jul 5. doi: 10.1111/j.1600-0897.2012.01172.x. [Epub ahead of print]
H1N1/09 Influenza A Virus Infection of Immortalized First Trimester Human Trophoblast Cell Lines.
Komine-Aizawa S, Suzaki A, Trinh QD, Izumi Y, Shibata T, Kuroda K, Hayakawa S.
Source
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Abstract
PROBLEM:
Although high maternal mortality was reported in the most recent pandemic of swine-origin influenza A H1N1/09 (H1N1/09), its direct effects on the feto-placental unit are unknown. In the present study, we examined the susceptibility of immortalized human trophoblasts to clinical isolates of pandemic 2009 H1N1 influenza A (H1N1/09) virus.
METHOD OF STUDY:
The H1N1/09 virus was isolated from a patient with influenza, sequenced and identified as the A/Narita/2009 (H1N1) strain. The trophoblast cell lines Swan71 and HTR8 were challenged with the virus and examined for the expression of H1N1/09 viral RNA and proteins.
RESULTS:
Viral RNA and proteins were observed 24 hr after inoculation. However, viral release was not detected.
CONCLUSION:
First trimester human trophoblast cell lines were susceptible to the H1N1/09 influenza A virus. However, viral release and cytopathic effects were minimal. Our data suggest that placental damage by the H1N1/09 virus may be limited.
? 2012 John Wiley & Sons A/S.
PMID:
22762384
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22762384
H1N1/09 Influenza A Virus Infection of Immortalized First Trimester Human Trophoblast Cell Lines.
Komine-Aizawa S, Suzaki A, Trinh QD, Izumi Y, Shibata T, Kuroda K, Hayakawa S.
Source
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Abstract
PROBLEM:
Although high maternal mortality was reported in the most recent pandemic of swine-origin influenza A H1N1/09 (H1N1/09), its direct effects on the feto-placental unit are unknown. In the present study, we examined the susceptibility of immortalized human trophoblasts to clinical isolates of pandemic 2009 H1N1 influenza A (H1N1/09) virus.
METHOD OF STUDY:
The H1N1/09 virus was isolated from a patient with influenza, sequenced and identified as the A/Narita/2009 (H1N1) strain. The trophoblast cell lines Swan71 and HTR8 were challenged with the virus and examined for the expression of H1N1/09 viral RNA and proteins.
RESULTS:
Viral RNA and proteins were observed 24 hr after inoculation. However, viral release was not detected.
CONCLUSION:
First trimester human trophoblast cell lines were susceptible to the H1N1/09 influenza A virus. However, viral release and cytopathic effects were minimal. Our data suggest that placental damage by the H1N1/09 virus may be limited.
? 2012 John Wiley & Sons A/S.
PMID:
22762384
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/22762384