tetano
Editor, Senior Moderator
Sci Rep. 2019 Aug 12;9(1):11616. doi: 10.1038/s41598-019-48076-6.
[h=1]Glycan-immobilized dual-channel field effect transistor biosensor for the rapid identification of pandemic influenza viral particles.[/h] Hideshima S[SUP]1,[/SUP][SUP]2[/SUP], Hayashi H[SUP]3[/SUP], Hinou H[SUP]4[/SUP], Nambuya S[SUP]3[/SUP], Kuroiwa S[SUP]5[/SUP], Nakanishi T[SUP]5[/SUP], Momma T[SUP]5,[/SUP][SUP]3[/SUP], Nishimura SI[SUP]4[/SUP], Sakoda Y[SUP]6[/SUP], Osaka T[SUP]7,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h] 1 Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumaki-cho, Shinjuku-ku, Tokyo, 162-0041, Japan. s.hideshima@ruri.waseda.jp. 2 Research Initiative for Supra-Materials, Interdisciplinary Cluster for Cutting Edge, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano, 386-8567, Japan. s.hideshima@ruri.waseda.jp. 3 Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan. 4 Faculty of Advanced Life Science, Hokkaido University, N21, W11, Kita-ku, Sapporo-shi, 001-0021, Hokkaido, Japan. 5 Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumaki-cho, Shinjuku-ku, Tokyo, 162-0041, Japan. 6 Faculty of Veterinary Medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo-shi, 060-0818, Hokkaido, Japan. 7 Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumaki-cho, Shinjuku-ku, Tokyo, 162-0041, Japan. osakatets@waseda.jp. 8 Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan. osakatets@waseda.jp.
[h=3]Abstract[/h] Pandemic influenza, triggered by the mutation of a highly pathogenic avian influenza virus (IFV), has caused considerable damage to public health. In order to identify such pandemic IFVs, antibodies that specifically recognize viral surface proteins have been widely used. However, since the analysis of a newly discovered virus is time consuming, this delays the availability of suitable detection antibodies, making this approach unsuitable for the early identification of pandemic IFVs. Here we propose a label-free semiconductor-based biosensor functionalized with sialic-acid-containing glycans for the rapid identification of the pandemic IFVs present in biological fluids. Specific glycans are able to recognize wild-type human and avian IFVs, suggesting that they are useful in discovering pandemic IFVs at the early stages of an outbreak. We successfully demonstrated that a dual-channel integrated FET biosensing system, which were modified with 6'-sialyllactose and 3'-sialyllactose for each gate area, can directly and specifically detect human H1N1 and avian H5N1 IFV particles, respectively, present in nasal mucus. Furthermore, to examine the possibility of identifying pandemic IFVs, the signal attributed to the detection of Newcastle disease virus (NDV) particles, which was selected as a prime model of a pandemic IFV, was clearly observed from both sensing gates. Our findings suggest that the proposed glycan-immobilized sensing system could be useful in identifying new pandemic IFVs at the source of an outbreak.
PMID: 31406167 DOI: 10.1038/s41598-019-48076-6
[h=1]Glycan-immobilized dual-channel field effect transistor biosensor for the rapid identification of pandemic influenza viral particles.[/h] Hideshima S[SUP]1,[/SUP][SUP]2[/SUP], Hayashi H[SUP]3[/SUP], Hinou H[SUP]4[/SUP], Nambuya S[SUP]3[/SUP], Kuroiwa S[SUP]5[/SUP], Nakanishi T[SUP]5[/SUP], Momma T[SUP]5,[/SUP][SUP]3[/SUP], Nishimura SI[SUP]4[/SUP], Sakoda Y[SUP]6[/SUP], Osaka T[SUP]7,[/SUP][SUP]8[/SUP].
[h=3]Author information[/h] 1 Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumaki-cho, Shinjuku-ku, Tokyo, 162-0041, Japan. s.hideshima@ruri.waseda.jp. 2 Research Initiative for Supra-Materials, Interdisciplinary Cluster for Cutting Edge, Shinshu University, 3-15-1 Tokida, Ueda-shi, Nagano, 386-8567, Japan. s.hideshima@ruri.waseda.jp. 3 Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan. 4 Faculty of Advanced Life Science, Hokkaido University, N21, W11, Kita-ku, Sapporo-shi, 001-0021, Hokkaido, Japan. 5 Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumaki-cho, Shinjuku-ku, Tokyo, 162-0041, Japan. 6 Faculty of Veterinary Medicine, Hokkaido University, N18, W9, Kita-ku, Sapporo-shi, 060-0818, Hokkaido, Japan. 7 Research Organization for Nano & Life Innovation, Waseda University, 513 Waseda-tsurumaki-cho, Shinjuku-ku, Tokyo, 162-0041, Japan. osakatets@waseda.jp. 8 Graduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan. osakatets@waseda.jp.
[h=3]Abstract[/h] Pandemic influenza, triggered by the mutation of a highly pathogenic avian influenza virus (IFV), has caused considerable damage to public health. In order to identify such pandemic IFVs, antibodies that specifically recognize viral surface proteins have been widely used. However, since the analysis of a newly discovered virus is time consuming, this delays the availability of suitable detection antibodies, making this approach unsuitable for the early identification of pandemic IFVs. Here we propose a label-free semiconductor-based biosensor functionalized with sialic-acid-containing glycans for the rapid identification of the pandemic IFVs present in biological fluids. Specific glycans are able to recognize wild-type human and avian IFVs, suggesting that they are useful in discovering pandemic IFVs at the early stages of an outbreak. We successfully demonstrated that a dual-channel integrated FET biosensing system, which were modified with 6'-sialyllactose and 3'-sialyllactose for each gate area, can directly and specifically detect human H1N1 and avian H5N1 IFV particles, respectively, present in nasal mucus. Furthermore, to examine the possibility of identifying pandemic IFVs, the signal attributed to the detection of Newcastle disease virus (NDV) particles, which was selected as a prime model of a pandemic IFV, was clearly observed from both sensing gates. Our findings suggest that the proposed glycan-immobilized sensing system could be useful in identifying new pandemic IFVs at the source of an outbreak.
PMID: 31406167 DOI: 10.1038/s41598-019-48076-6