tetano
Editor, Senior Moderator
Anal Chem. 2014 Jul 9. [Epub ahead of print]
Glycan based detection and drug susceptibility of influenza virus.
Dinh HT, Zhang X, Sweeney JB, Yang Y, He Y, Dhawane A, Iyer SS.
Abstract
We have developed a panel of synthetic glycans as receptor mimics for the specific capture of influenza viruses. The glycans were printed onto commercial glass slides using a free amine at the end of a spacer to generate a small focused microarray. The microarray was evaluated for its ability to capture three different strains of influenza A virus, two H1N1, A/Brisbane/59/2007 & A/Solomon Islands/3/2006 and one H3N2 A/Aichi/2/1968. We observed excellent detection ability with some compounds exhibiting clinically relevant (10<sup>plaque forming units) limit of detection. We also tested the drug susceptibility of current antivirals, Zanamivir? and Ostelamivir? using this microarray and could determine antiviral resistance for these strains.
PMID:
25007351
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25007351
Glycan based detection and drug susceptibility of influenza virus.
Dinh HT, Zhang X, Sweeney JB, Yang Y, He Y, Dhawane A, Iyer SS.
Abstract
We have developed a panel of synthetic glycans as receptor mimics for the specific capture of influenza viruses. The glycans were printed onto commercial glass slides using a free amine at the end of a spacer to generate a small focused microarray. The microarray was evaluated for its ability to capture three different strains of influenza A virus, two H1N1, A/Brisbane/59/2007 & A/Solomon Islands/3/2006 and one H3N2 A/Aichi/2/1968. We observed excellent detection ability with some compounds exhibiting clinically relevant (10<sup>plaque forming units) limit of detection. We also tested the drug susceptibility of current antivirals, Zanamivir? and Ostelamivir? using this microarray and could determine antiviral resistance for these strains.
PMID:
25007351
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/25007351