tetano
Editor, Senior Moderator
Genomics
. 2020 Nov 5;S0888-7543(20)32000-0.
doi: 10.1016/j.ygeno.2020.11.003. Online ahead of print.
Trends of mutation accumulation across global SARS-CoV-2 genomes: Implications for the evolution of the novel coronavirus
Chayan Roy[SUP] 1 [/SUP], Santi M Mandal[SUP] 2 [/SUP], Suresh K Mondal[SUP] 2 [/SUP], Shriparna Mukherjee[SUP] 3 [/SUP], Tarunendu Mapder[SUP] 4 [/SUP], Wriddhiman Ghosh[SUP] 5 [/SUP], Ranadhir Chakraborty[SUP] 6 [/SUP]
Affiliations
Abstract
To understand SARS-CoV-2 microevolution, this study explored the genome-wide frequency, gene-wise distribution, and molecular nature of all point-mutations detected across its 71,703 RNA-genomes deposited in GISAID till 21 August 2020. Globally, nsp1/nsp2 and orf7a/orf3a were the most mutation-ridden non-structural and structural genes respectively. Phylogeny of 4618 spatiotemporally-representative genomes revealed that entities belonging to the early lineages are mostly spread over Asian countries, including India, whereas the recently-derived lineages are more globally distributed. Of the total 20,163 instances of polymorphism detected across global genomes, 12,594 and 7569 involved transitions and transversions, predominated by cytidine-to-uridine and guanosine-to-uridine conversions, respectively. Positive selection of nonsynonymous mutations (dN/dS >1) in most of the structural, but not the non-structural, genes indicated that SARS-CoV-2 has already harmonized its replication/transcription machineries with the host metabolism, while it is still redefining virulence/transmissibility strategies at the molecular level. Mechanistic bases and evolutionary/pathogenicity-related implications are discussed for the predominant mutation-types.
Keywords: Genome-wide mutations; Microevolution; Nonsynonymous and synonymous mutations; SARS-CoV-2; Transition; Transversion.
. 2020 Nov 5;S0888-7543(20)32000-0.
doi: 10.1016/j.ygeno.2020.11.003. Online ahead of print.
Trends of mutation accumulation across global SARS-CoV-2 genomes: Implications for the evolution of the novel coronavirus
Chayan Roy[SUP] 1 [/SUP], Santi M Mandal[SUP] 2 [/SUP], Suresh K Mondal[SUP] 2 [/SUP], Shriparna Mukherjee[SUP] 3 [/SUP], Tarunendu Mapder[SUP] 4 [/SUP], Wriddhiman Ghosh[SUP] 5 [/SUP], Ranadhir Chakraborty[SUP] 6 [/SUP]
Affiliations
- PMID: 33161087
- DOI: 10.1016/j.ygeno.2020.11.003
Abstract
To understand SARS-CoV-2 microevolution, this study explored the genome-wide frequency, gene-wise distribution, and molecular nature of all point-mutations detected across its 71,703 RNA-genomes deposited in GISAID till 21 August 2020. Globally, nsp1/nsp2 and orf7a/orf3a were the most mutation-ridden non-structural and structural genes respectively. Phylogeny of 4618 spatiotemporally-representative genomes revealed that entities belonging to the early lineages are mostly spread over Asian countries, including India, whereas the recently-derived lineages are more globally distributed. Of the total 20,163 instances of polymorphism detected across global genomes, 12,594 and 7569 involved transitions and transversions, predominated by cytidine-to-uridine and guanosine-to-uridine conversions, respectively. Positive selection of nonsynonymous mutations (dN/dS >1) in most of the structural, but not the non-structural, genes indicated that SARS-CoV-2 has already harmonized its replication/transcription machineries with the host metabolism, while it is still redefining virulence/transmissibility strategies at the molecular level. Mechanistic bases and evolutionary/pathogenicity-related implications are discussed for the predominant mutation-types.
Keywords: Genome-wide mutations; Microevolution; Nonsynonymous and synonymous mutations; SARS-CoV-2; Transition; Transversion.