tetano
Editor, Senior Moderator
Vet Microbiol. 2013 Oct 29. pii: S0378-1135(13)00483-5. doi: 10.1016/j.vetmic.2013.10.010. [Epub ahead of print]
Genomic analysis of influenza A virus from captive wild boars in Brazil reveals a human-like H1N2 influenza virus.
Biondo N, Schaefer R, Gava D, Cant?o ME, Silveira S, Mores MA, Ciacci-Zanella JR, Barcellos DE.
Source
Federal University of Rio Grande do Sul/UFRGS, Porto Alegre, RS, Brazil.
Abstract
Influenza is a viral disease that affects human and several animal species. In Brazil, H1N1, H3N2 and 2009 pandemic H1N1 A(H1N1)pdm09 influenza A viruses (IAV) circulate in domestic swine herds. Wild boars are also susceptible to IAV infection but in Brazil until this moment there are no reports of IAV infection in wild boars or in captive wild boars populations. Herein the occurrence of IAV in captive wild boars with the presence of lung consolidation lesions during slaughter was investigated. Lung samples were screened by RT-PCR for IAV detection. IAV positive samples were further analyzed by quantitative real-time PCR (qRRT-PCR), virus isolation, genomic sequencing, histopathology and immunohistochemistry (IHC). Eleven out of 60 lungs (18.3%) were positive for IAV by RT-PCR and seven out of the eleven were also positive for A(H1N1)pdm09 by qRRT-PCR. Chronic diffuse bronchopneumonia was observed in all samples and IHC analysis was negative for influenza A antigen. Full genes segments of H1N2 IAV were sequenced using Illumina's genome analyzer platform (MiSeq). The genomic analysis revealed that the HA and NA genes clustered with IAVs of the human lineage and the six internal genes were derived from the H1N1pdm09 IAV. This is the first report of a reassortant human-like H1N2 influenza virus infection in captive wild boars in Brazil and indicates the need to monitor IAV evolution in Suidae populations.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Captive wild boars, Consolidation, H1N2, Influenza A virus, Reassortment
PMID:
24238665
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24238665
Genomic analysis of influenza A virus from captive wild boars in Brazil reveals a human-like H1N2 influenza virus.
Biondo N, Schaefer R, Gava D, Cant?o ME, Silveira S, Mores MA, Ciacci-Zanella JR, Barcellos DE.
Source
Federal University of Rio Grande do Sul/UFRGS, Porto Alegre, RS, Brazil.
Abstract
Influenza is a viral disease that affects human and several animal species. In Brazil, H1N1, H3N2 and 2009 pandemic H1N1 A(H1N1)pdm09 influenza A viruses (IAV) circulate in domestic swine herds. Wild boars are also susceptible to IAV infection but in Brazil until this moment there are no reports of IAV infection in wild boars or in captive wild boars populations. Herein the occurrence of IAV in captive wild boars with the presence of lung consolidation lesions during slaughter was investigated. Lung samples were screened by RT-PCR for IAV detection. IAV positive samples were further analyzed by quantitative real-time PCR (qRRT-PCR), virus isolation, genomic sequencing, histopathology and immunohistochemistry (IHC). Eleven out of 60 lungs (18.3%) were positive for IAV by RT-PCR and seven out of the eleven were also positive for A(H1N1)pdm09 by qRRT-PCR. Chronic diffuse bronchopneumonia was observed in all samples and IHC analysis was negative for influenza A antigen. Full genes segments of H1N2 IAV were sequenced using Illumina's genome analyzer platform (MiSeq). The genomic analysis revealed that the HA and NA genes clustered with IAVs of the human lineage and the six internal genes were derived from the H1N1pdm09 IAV. This is the first report of a reassortant human-like H1N2 influenza virus infection in captive wild boars in Brazil and indicates the need to monitor IAV evolution in Suidae populations.
Copyright ? 2013 Elsevier B.V. All rights reserved.
KEYWORDS:
Captive wild boars, Consolidation, H1N2, Influenza A virus, Reassortment
PMID:
24238665
[PubMed - as supplied by publisher]
http://www.ncbi.nlm.nih.gov/pubmed/24238665