tetano
Editor, Senior Moderator
Research
Genetic variations of nucleoprotein gene of influenza A viruses isolated from swine in Thailand
Nattakarn Thippamom email, Donreuthai Sreta email, Pravina Kitikoon email, Roongroje Thanawongnuwech email, Yong Poovorawan email, Apiradee Theamboonlers email, Kamol Suwannakarn email, Sujira Parchariyanon email, Sudarat Damrongwatanapokin email and Alongkorn Amonsin email
Virology Journal 2010, 7:185doi:10.1186/1743-422X-7-185
Published: 9 August 2010
Abstract (provisional)
Background
Influenza A virus causes severe disease in both humans and animals and thus, has a considerably impact on economy and public health. In this study, the genetic variations of the nucleoprotein (NP) gene of influenza viruses recovered from swine in Thailand were determined.
Results
Twelve influenza A virus specimens were isolated from Thai swine. All samples were subjected to nucleotide sequencing of the complete NP gene. Phylogenetic analysis was conducted by comparing the NP gene of swine influenza viruses with that of seasonal and pandemic human viruses and highly pathogenic avian viruses from Thailand (n=77). Phylogenetic analysis showed that the NP gene from different host species clustered in distinct host specific lineages. The NP gene of swine influenza viruses clustered in either Eurasian swine or Classical swine lineages. Genetic analysis of the NP gene suggested that swine influenza viruses circulating in Thailand display 4 amino acids unique to Eurasian and Classical swine lineages. In addition, the result showed 1 and 5 amino acids unique to avian and human lineages, respectively. Furthermore, nucleotide substitution rates showed that the NP gene is highly conserved especially in avian influenza viruses.
Conclusion
The NP gene sequence of influenza A in Thailand is highly conserved within host-specific lineages and shows amino acids potentially unique to distinct NP lineages. This information can be used to investigate potential interspecies transmission of influenza A viruses. In addition, the genetic variations of the NP gene will be useful for monitoring the viruses and preparing effective prevention and control strategies for potentially pandemic influenza outbreaks.
The complete article
http://www.virologyj.com/content/pdf/1743-422x-7-185.pdf
Genetic variations of nucleoprotein gene of influenza A viruses isolated from swine in Thailand
Nattakarn Thippamom email, Donreuthai Sreta email, Pravina Kitikoon email, Roongroje Thanawongnuwech email, Yong Poovorawan email, Apiradee Theamboonlers email, Kamol Suwannakarn email, Sujira Parchariyanon email, Sudarat Damrongwatanapokin email and Alongkorn Amonsin email
Virology Journal 2010, 7:185doi:10.1186/1743-422X-7-185
Published: 9 August 2010
Abstract (provisional)
Background
Influenza A virus causes severe disease in both humans and animals and thus, has a considerably impact on economy and public health. In this study, the genetic variations of the nucleoprotein (NP) gene of influenza viruses recovered from swine in Thailand were determined.
Results
Twelve influenza A virus specimens were isolated from Thai swine. All samples were subjected to nucleotide sequencing of the complete NP gene. Phylogenetic analysis was conducted by comparing the NP gene of swine influenza viruses with that of seasonal and pandemic human viruses and highly pathogenic avian viruses from Thailand (n=77). Phylogenetic analysis showed that the NP gene from different host species clustered in distinct host specific lineages. The NP gene of swine influenza viruses clustered in either Eurasian swine or Classical swine lineages. Genetic analysis of the NP gene suggested that swine influenza viruses circulating in Thailand display 4 amino acids unique to Eurasian and Classical swine lineages. In addition, the result showed 1 and 5 amino acids unique to avian and human lineages, respectively. Furthermore, nucleotide substitution rates showed that the NP gene is highly conserved especially in avian influenza viruses.
Conclusion
The NP gene sequence of influenza A in Thailand is highly conserved within host-specific lineages and shows amino acids potentially unique to distinct NP lineages. This information can be used to investigate potential interspecies transmission of influenza A viruses. In addition, the genetic variations of the NP gene will be useful for monitoring the viruses and preparing effective prevention and control strategies for potentially pandemic influenza outbreaks.
The complete article
http://www.virologyj.com/content/pdf/1743-422x-7-185.pdf